Development of the DNA-based biosensors for high performance in detection of molecular biomarkers: More rapid, sensitive, and universal

Wang, Qiong; Wang, Jing; Huang, Yan; Du, Yichen; Zhang, Yi; Cui, Yunxi; Kong, De‐Ming

doi:10.1016/j.bios.2021.113739

Cited by 50 publications

(24 citation statements)

References 190 publications

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“…The introduction of nanotechnology can overcome many limitations of traditional probes, greatly improve the sensitivity and other properties of biosensors, and promote the development of new types of biosensors, which are important for the early diagnosis of cancer and cardiovascular diseases. , As an emerging class of building blocks of nanomaterials, nucleic acid has the advantages of programmability, compatibility, and good biocompatibility. By introducing chemical modifications, a series of molecules with unnatural backbones or nucleic acid bases can be synthesized.…”

mentioning

confidence: 99%

Oxidative Cleavage-Based Three-Dimensional DNA Biosensor for Ratiometric Detection of Hypochlorous Acid and Myeloperoxidase

Wang

et al. 2021

Anal. Chem.

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Methods to detect and quantify disease biomarkers with high specificity and sensitivity in biological fluids play a key role in enabling clinical diagnosis, including point-of-care testing. Myeloperoxidase (MPO) is an emerging biomarker for the detection of inflammation, neurodegenerative diseases, and cardiovascular disease, where excess MPO can lead to oxidative damage to biomolecules in homeostatic systems. While numerous methods have been developed for MPO analysis, most techniques are challenging in clinical applications due to the lack of amplification methods, high cost, or other practical drawbacks. Enzyme-linked immunosorbent assays are currently used for the quantification of MPO in clinical practice, which is often limited by the availability of antibodies with high affinity and specificity and the significant nonspecific binding of antibodies to the analytical surface. In contrast, nucleic acid-based biosensors are of interest because of their simplicity, fast response time, low cost, high sensitivity, and low background signal, but detection targets are limited to nucleic acids and non-nucleic acid biomarkers are rare. Recent studies reveal that the modification of a genome in the form of phosphorothioate is specifically sensitive to the oxidative effects of the MPO/H 2 O 2 /Cl − system. We developed an oxidative cleavage-based threedimensional DNA biosensor for rapid, ratiometric detection of HOCl and MPO in a "one-pot" method, which is simple, stable, sensitive, specific, and time-saving and does not require a complex reaction process, such as PCR and enzyme involvement. The constructed biosensor has also been successfully used for MPO detection in complex samples. This strategy is therefore of great value in disease diagnosis and biomedical research.

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confidence: 99%

Oxidative Cleavage-Based Three-Dimensional DNA Biosensor for Ratiometric Detection of Hypochlorous Acid and Myeloperoxidase

Wang

et al. 2021

Anal. Chem.

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“…Due to their multiple advantages, such as specificity, sensitivity, biocompatibility, and cost-effectivity, DNA-based biosensors are used in several fields, including disease prognosis, clinical diagnosis, food control, and environmental screening [126,128].…”

Section: Dna-based Biosensorsmentioning

confidence: 99%

“…Several studies have illustrated the ability of DNA-based biosensors to detect traces of heavy metals in the environment [125,[128][129][130]. In this case, the working principle is based on the affinity of some heavy metal ions toward forming stable duplex structures together with certain DNA bases.…”

Section: Dna-based Biosensorsmentioning

confidence: 99%

Recent Trends in Biosensors for Environmental Quality Monitoring

Gavrilaș

Ursachi

Perța-Crișan

et al. 2022

Sensors

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The monitoring of environmental pollution requires fast, reliable, cost-effective and small devices. This need explains the recent trends in the development of biosensing devices for pollutant detection. The present review aims to summarize the newest trends regarding the use of biosensors to detect environmental contaminants. Enzyme, whole cell, antibody, aptamer, and DNA-based biosensors and biomimetic sensors are discussed. We summarize their applicability to the detection of various pollutants and mention their constructive characteristics. Several detection principles are used in biosensor design: amperometry, conductometry, luminescence, etc. They differ in terms of rapidity, sensitivity, profitability, and design. Each one is characterized by specific selectivity and detection limits depending on the sensitive element. Mimetic biosensors are slowly gaining attention from researchers and users due to their advantages compared with classical ones. Further studies are necessary for the development of robust biosensing devices that can successfully be used for the detection of pollutants from complex matrices without prior sample preparation.

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“…Biosensor-based diagnostic represents an advanced technology in pursuit of rapid detection of various analytes including pathogens [12,13]. A typical biosensor is composed of immobilized bio-sensitive materials as recognition moieties and physical or chemical transducers that translate the recognition information into measurable signals.…”

Section: Introductionmentioning

confidence: 99%

Aptasensors for the detection of infectious pathogens: design strategies and point-of-care testing

2022

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The epidemic of infectious diseases caused by contagious pathogens is a life-threatening hazard to the entire human population worldwide. A timely and accurate diagnosis is the critical link in the fight against infectious diseases. Aptamer-based biosensors, the so-called aptasensors, employ nucleic acid aptamers as bio-receptors for the recognition of target pathogens of interest. This review focuses on the design strategies as well as state-of-the-art technologies of aptasensor-based diagnostics for infectious pathogens (mainly bacteria and viruses), covering the utilization of three major signal transducers, the employment of aptamers as recognition moieties, the construction of versatile biosensing platforms (mostly micro and nanomaterial-based), innovated reporting mechanisms, and signal enhancement approaches. Advanced point-of-care testing (POCT) for infectious disease diagnostics are also discussed highlighting some representative ready-to-use devices to address the urgent needs of currently prevalent coronavirus disease 2019 (COVID-19). Pressing issues in aptamer-based technology and some future perspectives of aptasensors are provided for the implementation of aptasensor-based diagnostics into practical application.

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Development of the DNA-based biosensors for high performance in detection of molecular biomarkers: More rapid, sensitive, and universal

Cited by 50 publications

References 190 publications

Oxidative Cleavage-Based Three-Dimensional DNA Biosensor for Ratiometric Detection of Hypochlorous Acid and Myeloperoxidase

Oxidative Cleavage-Based Three-Dimensional DNA Biosensor for Ratiometric Detection of Hypochlorous Acid and Myeloperoxidase

Recent Trends in Biosensors for Environmental Quality Monitoring

Aptasensors for the detection of infectious pathogens: design strategies and point-of-care testing

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