Development of the Simple Gene Gun Apparatuses Systems

Abumhadi, N.; Kamenarova, Kunka; Todorovska, E.; Dimov, G.; Takumi, Shigeo; Nakamura, Chiharu; Anzai, Hiroyuki; Atanassov, A.

doi:10.1080/13102818.2005.10817197

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…In respect to the promoter activity, our results were in correspondence with other authors (5,71) who reported that the rice Act1 promoter activates gus gene more profoundly than the Ubi1 promoter in barley tissues.…”

Section: Resultssupporting

confidence: 92%

Production of Recombinant Human Lactoferin in Transgenic Barley

Kamenarova

Gecheff

Stoyanova

et al. 2007

Biotechnology & Biotechnological Equipment

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Section: Resultssupporting

confidence: 92%

Production of Recombinant Human Lactoferin in Transgenic Barley

Kamenarova

Gecheff

Stoyanova

et al. 2007

Biotechnology & Biotechnological Equipment

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“…Our construct contained the ubiquitin (ubi) promoter and the tm1' terminator from maize controlling the expression of the HsEPO cDNA. The maize ubi 5′-sequence is known to be a very strong promoter in monocotyledonous (monocot) cells, but less effective in dicotyledonous (dicot) cells [35][36][37][38][39][40][41][42]. We used this promoter to control the expression of the HsEPO cDNA in rice (a monocot) and tobacco (a dicot).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Transgenic Expression of a Synthetic Human Erythropoietin Gene in Tobacco

Sperb

Werlang

Margis‐Pinheiro

et al. 2011

Appl Biochem Biotechnol

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Erythropoietin (EPO) is a hormone belonging to a group of hematopoietic growth factors that control the proliferation and differentiation of bone marrow cells. It induces the production of erythrocytes, thereby increasing the amount of circulating hemoglobin and oxygen. Previous attempts to transgenically express human EPO in plants failed to succeed because the plants exhibited abnormal morphology and infertility. In the present work, we describe the generation of fertile transgenic tobacco plants able to express a synthetic version of human EPO. A 582-bp fragment of the human EPO gene was synthesized using a PCR-based method and ligated into pCR-Blunt. After sequencing, the human EPO fragment was transferred to pWUbi.tm1 and the expression cassette was then transferred to the binary vector pWBVec4a. After Agrobacterium-mediated transformation of Nicotiana tabacum SR1 plants, integration of the transgene into T(0) and T(1) plant genomes was confirmed by PCR. The human EPO gene was found to be expressed in tobacco leaves at the mRNA and protein levels. Self-crossing allowed us to obtain T(1) plants exhibiting Mendelian segregation of the transgene. None of the plants presented any kind of malformation or deformity.

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