Development of therapeutic proteins: advances and challenges

Akash, Muhammad Sajid Hamid; Rehman, Kanwal; Tariq, Muhammad; Chen, Shuqing

doi:10.3906/biy-1411-8

Cited by 42 publications

(20 citation statements)

References 153 publications

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“…Among the 15 best-selling drugs in 2018, 11 were biopharmaceuticals. 1 However, therapies with biopharmaceuticals also have their drawbacks, such as limited cell viability, short half-life after administration (Laflamme et al, 2007), immunogenicity, low stability, and degradation (Akash et al, 2015). One of the ways to overcome these issues is to incorporate the biopharmaceuticals into nanofibers.…”

Section: Biological Products In Nanofibersmentioning

confidence: 99%

Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications

Stojanov

Berlec

2020

Front. Bioeng. Biotechnol.

129

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Electrospinning is a technique that uses polymer solutions and strong electric fields to produce nano-sized fibers that have wide-ranging applications. We present here an overview of the use of electrospinning to incorporate biological products into nanofibers, including microorganisms, cells, proteins, and nucleic acids. Although the conditions used during electrospinning limit the already problematic viability/stability of such biological products, their effective incorporation into nanofibers has been shown to be feasible. Synthetic polymers have been more frequently applied to make nanofibers than natural polymers. Polymer blends are commonly used to achieve favorable physical properties of nanofibers. The majority of nanofibers that contain biological product have been designed for therapeutic applications. The incorporation of these biological products into nanofibers can promote their stability or viability, and also allow their delivery to a desired tissue or organ. Other applications include plant protection in agriculture, fermentation in the food industry, biocatalytic environmental remediation, and biosensing. Live cells that have been incorporated into nanofibers include bacteria and fungi. Nanofibers have served as scaffolds for stem cells seeded on a surface, to enable their delivery and application in tissue regeneration and wound healing. Viruses incorporated into nanofibers have been used in gene delivery, as well as in therapies against bacterial infections and cancers. Proteins (hormones, growth factors, and enzymes) and nucleic acids (DNA and RNA) have been incorporated into nanofibers, mainly to treat diseases and enhance their stability. To summarize, incorporation of biological products into nanofibers has numerous advantages, such as providing protection and facilitating controlled delivery from a solid form with a large surface area. Future studies should address the challenge of transferring nanofibers with biological products into practical and industrial use.

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Section: Biological Products In Nanofibersmentioning

confidence: 99%

Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications

Stojanov

Berlec

2020

Front. Bioeng. Biotechnol.

129

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“…The most common of these include encapsulation within inert and biocompatible polymers. By using enzyme inhibitors degradation processes can also be prevented [53].…”

Section: Protein Degradationmentioning

confidence: 99%

“…IL-1Ra is a naturally occurring anti-inflammatory antagonist of pro-inflammatory cytokines, which have been successfully encapsulated in PF127. This polymer provides sustained release and enhances the stability and therapeutical activity [53].…”

Section: Metabolism and Eliminationmentioning

confidence: 99%

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A Review on Recent Advances in Stabilizing Peptides/Proteins upon Fabrication in Hydrogels from Biodegradable Polymers

Raza

Zafar

Zhu

et al. 2017

Preprint

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Hydrogels evolved as an outstanding carrier material for local and controlled drug delivery that tend to the shortcomings of old conventional dosage forms for small drugs (NSAIDS) and large peptides and proteins. Aqueous swellable and crosslinked polymeric network structure of hydrogels is composed of various natural, synthetic and semisynthetic biodegradable polymers. Hydrogels have remarkable properties of functionality, reversibility, sterilizability, and biocompatibility. All these dynamic properties of hydrogels have increased the interest in their use as a carrier for peptides and proteins to be released slowly in a sustained manner. The therapeutical peptide and proteins are remarkable therapeutic agents in today's world that allows the treatment of severe, chronic and life-threatening diseases, such as diabetes, rheumatoid arthritis, hepatitis in an easy manner. Despite few limitations, hydrogels provide fine tuning of proteins and peptides delivery with enormous impact in clinical medicine. The primary objective of this article is to review current issues concerned with the therapeutics peptides and proteins and impact of remarkable properties of hydrogels on these therapeutic agents. Different routes for pharmaceutical peptides and proteins and superiority over other drugs candidates are presented. The article will also review literature concerning classification of hydrogels on different basis, polymers used, release mechanisms their physical and chemical characteristics and diverse applications.

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“…Recombinant therapeutic proteins, drug-antibody mixed, vaccines, enzymes, recombinant/normal cytokines, interferons, monoclonal antibodies, growth hormones, and coagulation factors are known as biochemical therapists. They have been proven effective in the treatment of many potentially fatal diseases, such as cancer, diabetes, and cardiac disorders [2]. Protein purification is essential for basic protein research and the production of therapist antibodies [3][4][5], and the expansion of the need for pure protein [6] is challenging the existing purification methods [7].…”

Section: Introductionmentioning

confidence: 99%

Membrane and Bioseparation

Mansourpanah¹,

Emamian²

2020

Advances in Membrane Technologies

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Although one of the strongest methods of purification is chromatography, the major problem of porous bed chromatography is that purification takes place using the diffusion. This will prolong the purification process and bring down the efficiency. In recent years, membrane methods have greatly overcome this limitation due to low membrane thickness, low pressure drop, and convective flow, and they are a great alternative to chromatography columns. Unfortunately, the membranes have a low surface area. For solving such problem, membrane modification with polymeric brushes and layer-by-layer adsorption in polyelectrolyte films can be attractive. Accordingly, in this chapter we introduce types of biomolecule purification methods, the best purification method, membrane modification techniques, and their limitations and assets. Also, we introduce the membrane as an attractive tool for selective purification and separation of biomolecules.

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Development of therapeutic proteins: advances and challenges

Cited by 42 publications

References 153 publications

Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications

Electrospun Nanofibers as Carriers of Microorganisms, Stem Cells, Proteins, and Nucleic Acids in Therapeutic and Other Applications

A Review on Recent Advances in Stabilizing Peptides/Proteins upon Fabrication in Hydrogels from Biodegradable Polymers

Membrane and Bioseparation

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