Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests

Poveda, Jorge; Francisco, Marta; Cartea, María Elena; Velasco, Pablo

doi:10.3390/plants9121664

Cited by 22 publications

(9 citation statements)

References 144 publications

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“…Alternaria spp., Fusarium oxysporum , Albuga candia , and Leptosphaeria maculans are the most prevalent fungal diseases that affect Brassica [ 36 ]. Brassica crops are susceptible to bacterial rot disease as well [ 37 ]. Similarly, salinity and drought among abiotic stresses have the highest impact on Brassica yield [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

Analyses of Lysin-motif Receptor-like Kinase (LysM-RLK) Gene Family in Allotetraploid Brassica napus L. and Its Progenitor Species: An In Silico Study

Abedi

Hajiahmadi

Kordrostami

et al. 2021

Cells

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The LysM receptor-like kinases (LysM-RLKs) play a crucial role in plant symbiosis and response to environmental stresses. Brassica napus, B. rapa, and B. oleracea are utilized as valuable vegetables. Different biotic and abiotic stressors affect these crops, resulting in yield losses. Therefore, genome-wide analysis of the LysM-RLK gene family was conducted. From the genome of the examined species, 33 LysM-RLK have been found. The conserved domains of Brassica LysM-RLKs were divided into three groups: LYK, LYP, and LysMn. In the Brassica LysM-RLK gene family, only segmental duplication has occurred. The Ka/Ks ratio for the duplicated pair of genes was less than one indicating that the genes’ function had not changed over time. The Brassica LysM-RLKs contain 70 cis-elements, indicating that they are involved in stress response. 39 miRNA molecules were responsible for the post-transcriptional regulation of 12 Brassica LysM-RLKs. A total of 22 SSR loci were discovered in 16 Brassica LysM-RLKs. According to RNA-seq data, the highest expression in response to biotic stresses was related to BnLYP6. According to the docking simulations, several residues in the active sites of BnLYP6 are in direct contact with the docked chitin and could be useful in future studies to develop pathogen-resistant B. napus. This research reveals comprehensive information that could lead to the identification of potential genes for Brassica species genetic manipulation.

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Section: Introductionmentioning

confidence: 99%

Analyses of Lysin-motif Receptor-like Kinase (LysM-RLK) Gene Family in Allotetraploid Brassica napus L. and Its Progenitor Species: An In Silico Study

Abedi

Hajiahmadi

Kordrostami

et al. 2021

Cells

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“…The chitinases are glycosyl hydrolases that degrade chitin. As this polymer is the second most abundant on the Earth’s surface, these enzymes fulfil a varied number of functions in nature [ 181 , 182 ]; for instance, they participate in the defense mechanisms of some plants and other organisms in response to infections caused by pathogenic fungi [ 183 , 184 ]. Chitin’s chemical degradation can lead to the production of its deacetylated form, the polysaccharide chitosan, which is further hydrolyzed by chitosanases ( Figure 13 ).…”

Section: Chemical Classes Of Metabolitesmentioning

confidence: 99%

Cyanobacteria: A Promising Source of Antifungal Metabolites

et al. 2023

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Cyanobacteria are a rich source of secondary metabolites, and they have received a great deal of attention due to their applicability in different industrial sectors. Some of these substances are known for their notorious ability to inhibit fungal growth. Such metabolites are very chemically and biologically diverse. They can belong to different chemical classes, including peptides, fatty acids, alkaloids, polyketides, and macrolides. Moreover, they can also target different cell components. Filamentous cyanobacteria have been the main source of these compounds. This review aims to identify the key features of these antifungal agents, as well as the sources from which they are obtained, their major targets, and the environmental factors involved when they are being produced. For the preparation of this work, a total of 642 documents dating from 1980 to 2022 were consulted, including patents, original research, review articles, and theses.

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“…Further research utilizing RT-PCR validated that these chitinase genes were induced after wounding and exogenous treatments of jasmonic acid and salicylic acid similar to Alternaria infection [103]. A recent review summarized that the chitinases, glucanases or cry proteins provide broad-spectrum resistance against some major diseases including Alternaria blight and blackleg [104].…”

Section: Genetic Transformation For Alternaria Resistancementioning

confidence: 99%

Epidemiology, Genetics and Resistance of Alternaria Blight in Oilseed Brassica

Jyoti¹,

Sultana²,

Hassan³

et al. 2021

Brassica Breeding and Biotechnology

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Alternaria blight is one of the most deadly diseases of oilseed Brassica. This recalcitrant disease causes up to 50% yield loss across the globe. The disease is mainly caused by Alternaria brassicae and Alternaria brassicicola. These pathogens lack sexual stages and survive as conidia or condiospores on the debris of previous crops and susceptible weeds. Developing resistant oilseed Brassica cultivars to this disease has become a prime concern for researchers over the years. In absence of resistant oilseed Brassica cultivar, identification and introgression of resistance related genes can be a potential source for Alternaria blight resistance. As resistance toward Alternaria blight is governed by polygenes, intercrossing between the tolerant genotypes and subsequent selection will be the most appropriate way to transfer the quantitative resistance. For that reason, future breeding goal should focus on screening of germplasms for selecting genotypes containing resistance genes and structural features that favors resistance, like thick epicuticular wax, biochemical components such as phenols, phytoalexins and lower soluble sugars, reducing sugars and soluble nitrogen. Selected genotypes should be brought under appropriate breeding programs for attaining Alternaria blight resistance.

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Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests

Cited by 22 publications

References 144 publications

Analyses of Lysin-motif Receptor-like Kinase (LysM-RLK) Gene Family in Allotetraploid Brassica napus L. and Its Progenitor Species: An In Silico Study

Analyses of Lysin-motif Receptor-like Kinase (LysM-RLK) Gene Family in Allotetraploid Brassica napus L. and Its Progenitor Species: An In Silico Study

Cyanobacteria: A Promising Source of Antifungal Metabolites

Epidemiology, Genetics and Resistance of Alternaria Blight in Oilseed Brassica

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