Development of Ultra-High-Performance Liquid Chromatography Method for the Determination of Captopril in Pharmaceutical Dosage Forms

Logoyda, Liliya; Kondratova, Yuliya; Кorobko, Dmytro; Soroka, Yuriy

doi:10.22159/ajpcr.2017.v10i11.20462

Cited by 12 publications

(12 citation statements)

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“…In the present study, optimization and critical evaluation of mobile phase composition (gradient), flow rate, and analytical column were important to obtain good resolution of peaks of interest from the endogenous components, which, in turn, affect reproducibility and sensitivity of the method [4][5][6]. The resolution of peaks was best achieved with Discovery C18, 50×2.…”

Section: Resultsmentioning

confidence: 98%

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Logoyda

2018

Asian J Pharm Clin Res

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Objective: A simple, rapid high-performance liquid chromatography-mass spectrometry/mass spectrometry (MS/MS) method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution. . The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and to 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.400 ml/min into the MS electrospray ionization chamber. The sample volume was 5 µl.Results: Under these conditions, amlodipine was eluted at 1.61 min. According to the Caco-2 test results, amlodipine appeared to have moderate to high permeability. Recovery value for amlodipine is 52.33%. Caco-2 permeability values for amlodipine are in agreement with BCS Class I classification for these drugs and their known high bioavailability in humans. Conclusion:A new rapid method was developed for the determination of amlodipine from confluent Caco-2 monolayers and from aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for the examination of amlodipine from Caco-2 cell monolayers.

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Section: Resultsmentioning

confidence: 98%

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Logoyda

2018

Asian J Pharm Clin Res

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“…In the present study, optimization and critical evaluation of mobile phase composition, flow rate, and analytical column were important to obtain good resolution of peaks of interest from the endogenous components, which, in turn, affect reproducibility and sensitivity of the method [7][8][9][10][11][12][13]. The resolution of peaks was best achieved with Discovery C18, 50×2.1 mm, and 5 μm column.…”

Section: Resultsmentioning

confidence: 99%

A High-Performance Liquid Chromatography-Mass Spectrometry Method Development for the Quantitative Determination of Enalapril Maleate From Caco-2 Cell Monolayers

Logoyda

2018

Asian J Pharm Clin Res

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Objective: A simple, rapid high-performance liquid chromatography-mass spectrometry (HPLC MS/MS) method was developed for the determination of enalapril maleate from confluent Caco-2 monolayers and aqueous solution.Methods: Chromatography was achieved on Discovery C18, 50×2.1 mm, 5 μm column. Samples were chromatographed in a gradient mode (eluent A [acetonitrile-water-formic acid, 5: 95:0.1 v/v] and eluent B [acetonitrile-formic acid, 100:0.1 v/v]). The initial content of the eluent B is 0%, which increases linearly by 1.0 min to 100% and 1.01 min returns to the initial 0%. The mobile phase was delivered at a flow rate of 0.4 ml/min into the mass spectrometer ESI chamber. The sample volume was 5 μl.Results: Under these conditions, enalapril maleate was eluted at 1.52 min. According to the Caco-2 test results, enalapril showed low permeability. It should be noted that the recovery value for enalapril is 100.62 %. Low in vitro permeability data for enalapril are in good agreement with the literature data.Conclusion: From results of the analysis, it can be concluded that developed method is simple and rapid for determination of enalapril maleate from confluent Caco-2 monolayers and aqueous solution. Acquired results demonstrate that proposed strategy can be effortlessly and advantageously applied for examination of enalapril from Caco-2 cell monolayers.

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“…In previous studies, [3][4][5][6][7][8] specific, sensitive, and rapid HPLC methods had been developed and validated for the quantification of captopril in dosage forms and in human plasma. We have not found any study which shows optimal conditions for extraction of captopril by organic solvents from water solutions in dependence on pH solutions using UHPLC.…”

Section: Discussionmentioning

confidence: 99%

“…Assay performed using previously developed conditions. [3][4][5][6][7][8] Research of captopril extraction conditions with aqueous solutions of organic solvents was performed by the following procedure: The number of separating funnels made in 8.00 ml of buffer solutions and in 2.00 ml of standard solution of captopril; to the resulting mixture was added 10.00 ml appropriate organic solvent. Mixtures shaken in separating funnel for 5 min and left for 10 min to separate the layers.…”

Section: Methodsmentioning

confidence: 99%

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Logoyda¹

2018

AJP

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Introduction:In recent years, the principle and theory behind solvent extraction has gained wide acceptance as one of the new tools of modern pharmaceutical analysis. Analytical chemists have been using solvent extraction in the laboratory for many years for extracting molecular species from aqueous solutions by organic solvents. However, the requirements and objectives of an analytical procedure are quite different from those of a profit producing mill flow sheet. Depending on the nature, aggregate state, sample concentration and method of analysis, and various sampling procedures are used: Moisture removal, grinding, decomposition, dissolution, melting, elution, removal of the matrix, dilution, concentration, etc. The objective of this research was to select the optimal conditions for the extraction for captopril by organic solvents from water solutions in dependence on pH solutions. Materials and Methods: The chromatographic analysis of captopril performed on liquid chromatography ACQUITY Arc system. Results: The obtained data testify that the optimal solvents for the extraction of captopril in the process of separating it from objects of biological origin are chloroform and methylene chloride. The amount of substance released by chloroform is 91.48% (at pH 1), methylene chloride is 97.97% (at pH 1), and hexane is 69.65% (at pH 2). Conclusion: The extraction of captopril by organic solvents from water solutions in dependence on pH solutions has been conducted. As a result of studies, we have found that the optimal extragent is methylene chloride, which is extracted at pH 1-97.97% and chloroform is extracted at pH 1-91.48%.

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Development of Ultra-High-Performance Liquid Chromatography Method for the Determination of Captopril in Pharmaceutical Dosage Forms

Cited by 12 publications

References 2 publications

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

Quantitative Determination of Amlodipine From Caco-2 Cell Monolayers by High-Performance Liquid Chromatography–mass Spectrometry/Mass Spectrometry

A High-Performance Liquid Chromatography-Mass Spectrometry Method Development for the Quantitative Determination of Enalapril Maleate From Caco-2 Cell Monolayers

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