Development of ultraviolet-polymerizable enzyme pastes: bioprocess applications of screen-printed L-lactate sensors

Rohm, Ingrid; Genrich, Meike; Collier, W. A.; Bilitewski, Ursula

doi:10.1039/an9962100877

Cited by 51 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The electrodes were fabricated according to the process described by Rohm et al [20]. The layout is shown in Figure 1.…”

Section: Sensor Preparationmentioning

confidence: 99%

See 1 more Smart Citation

Recycling Systems Based on Screen-Printed Electrodes

Lisdat

Wollenberger

et al. 1998

Electroanalysis

Self Cite

View full text Add to dashboard Cite

Screen-printed enzyme electrodes sensitive to catecholamines were developed. The bioelectrocatalytic recycling systems investigated exploited the combination of laccase catalyzed catecholamine oxidation with the electrochemical reduction of the formed quinoic product or, alternatively, electrochemical oxidation of the catecholamine followed by quinoprotein glucose dehydrogenase (GDH) catalyzed reduction. Both systems allowed quantitation of nanomolar concentrations. The lower detection limit for dopamine was 50 nmol/L and 2 nmol/L for the laccase or GDH based system, respectively. The conditions for attaining the highest sensitivity were optimized. Relatively large oxygen fluctuations in solution can be tolerated without disturbing sensor performance. Good long-term stability can be provided for sensor operation and storage.

show abstract

“…The electrodes were fabricated according to the process described by Rohm et al [20]. The layout is shown in Figure 1.…”

Section: Sensor Preparationmentioning

confidence: 99%

“…This technique is not only suited for the fabrication of base electrodes, but also of whole sensor chips including the biocomponent [20,21]. The fabrication method is cheap and rather simple leading to robust thick film sensors.…”

Section: Introductionmentioning

confidence: 99%

Recycling Systems Based on Screen-Printed Electrodes

Lisdat

Wollenberger

et al. 1998

Electroanalysis

Self Cite

View full text Add to dashboard Cite

show abstract

“…Alginates and polyacrylamide gels are frequently used for this task. Methacrylate derivatives, as well as a number of other chemicals, permit polymerization by UV light, while pyrrole derivatives can be electrochemically polymerized (Rohm et al 1996;Cosnier 2000;Brahim et al 2001). The trapping of cells in polymer structures may create diffusion barriers, which could hinder the transport of analytes, resulting in prolonged washing, equilibration, and measuring steps.…”

Section: Immobilization Techniquesmentioning

confidence: 99%

Biosensors: new approaches in drug discovery

Keusgen

2002

Naturwissenschaften

120

View full text Add to dashboard Cite

The development of biosensors for analytical purposes has attracted a great deal of attention in recent years. A biosensor is defined as an analytical device consisting of a biological component (e.g., enzyme, antibody, entire cell, DNA) and a physical transducer (e.g., electrode, optical device). Biosensors are mostly designed for routine analysis, such as clinical diagnosis, quality control of food, in-process control of fermentations, and in environmental analysis. Many of these sensors are also suitable for screening purposes in order to find new drugs. Such systems should yield information either about compounds with known bioactivity or about the bioactivity of samples with known or unknown chemical composition. Biosensors intended for the latter purpose are essentially based on whole cells carrying receptors and ion channels at their surfaces. Miniaturization of structures, primarily based on silicon, allows integration of many sensors into arrays, which may be suitable for the screening of natural and chemical products as well as combinatorial libraries. Until now, no commercially available sensors of this kind exist but they are expected in the near future. Different biosensors, based on enzymes, antibodies, cells, artificial membranes and entire animal tissues, which can be used in drug discovery and may lead to efficient screening systems in the future, are described in this review.

show abstract

“…The composition of pastes and details of their fabrication procedure influence the electrochemical performance of the resulting electrode and have to be adapted with respect to the intended application. Platinum pastes are usually used for the fabrication of working electrodes used for the oxidation of hydrogen peroxide [21,22,23,24,25] or the reduction of oxygen [26], as the potential required for oxidation of hydrogen peroxide at graphite composites is rather high [27]. Carbonaceous pastes or inks are used when the enzyme or antibody is entrapped within the paste [17,28,29], direct electron transfer between enzyme and electrode is used [30,31] or the detection is based on compounds showing favorable electrochemistry at carbon electrodes.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical characterization of screen-printed carbonaceous electrodes for the determination of peroxidase activity in novel screen-printed flow-through modules

Stiene

Bilitewski

2002

Analytical and Bioanalytical Chemistry

Self Cite

View full text Add to dashboard Cite

A novel totally screen-printed flow-through cell for immunoanalysis is presented. It contained screen-printed carbonaceous electrodes, which allowed the determination of peroxidase activity through the electrochemical reduction of p-benzoquinone. As different electrode materials differ strongly in their electrochemical properties, electrodes resulting from various screen-printable carbonaceous pastes were characterized using the hydroquinone/ p-benzoquinone redox couple. For most of the electrodes, cyclic voltammogram peak separations of between 550 and 670 mV were observed indicating only quasi-reversible electrochemical behavior. This was confirmed by variation of the peak separation with scan rate. Heterogeneous electron transfer rates of ca. 0.5 - 1 x 10(-3) cm s(-1) and electrochemical activation energies of ca. 20 kJ mol(-1) were found. These flow-through cells were not only applied to electrochemical peroxidase activity determinations but also, in combination with a separate detector, as affinity reactors. After biotinylation of screen-printed layers, streptavidin and then biotinylated peroxidase could be bound. However, as signals were only 10-20% of those obtained with a column filled with biotinylated glass beads, only the screen-printed electrochemical detector was applied to the detection of antibodies against the African Swine Fever Virus.

show abstract

Development of ultraviolet-polymerizable enzyme pastes: bioprocess applications of screen-printed L-lactate sensors

Cited by 51 publications

References 12 publications

Recycling Systems Based on Screen-Printed Electrodes

Recycling Systems Based on Screen-Printed Electrodes

Biosensors: new approaches in drug discovery

Electrochemical characterization of screen-printed carbonaceous electrodes for the determination of peroxidase activity in novel screen-printed flow-through modules

Contact Info

Product

Resources

About