Development of UPLC-Q-TOF-MS Coupled with Cation-exchange Solid-phase Extraction Method for the Determination of Ten Pyrrolizidine Alkaloids in Herbal Medicines

Luo, Zhimin; Li, Xuan; Wang, Lu; Chang, Chun; Fu, Qiang

doi:10.2116/analsci.19p230

Cited by 13 publications

(4 citation statements)

References 42 publications

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“…c) analysis of ascorbic acid and dehydroascorbic acid in liquid and solid vegetable samples 30 . d) for detection of pyrrlozidine alkaloids in herbal medicines 31 .…”

Section: Examples: A)mentioning

confidence: 99%

A Mini-review on Ultra Performance Liquid Chromatography

et al. 2021

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Chromatography is a widely used analytical tool for separating a mixture of compounds into individual component. High performance liquid chromatography (HPLC) is one of the most important methods used for the separation, identification and quantification of a compounds present in a mixture. It meets many criteria of analysis but its main drawbacks are it is relatively time consuming to run a chromatogram and consumes high amount of solvent compared to other analytical methods. There is a need to develop a method which can overcome these drawbacks of HPLC. Ultra performance liquid chromatography (UPLC) is the new approach which opens novel direction in the field of liquid chromatography. It works on similar principle but shows better performance than conventional HPLC. UPLC is a technique of liquid chromatography with improved runtime and sensitivity with less than 2 μm particle size. The UPLC separation process is carried out under very high pressure (up to100 MPa). Additionally, it reduces the cost of reagent with shorter run time as compared to conventional HPLC. This article updated until 2020, provides a general review on the principle, instrumentation and application of UPLC in different fields of science.

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“…c) analysis of ascorbic acid and dehydroascorbic acid in liquid and solid vegetable samples 30 . d) for detection of pyrrlozidine alkaloids in herbal medicines 31 .…”

Section: Examples: A)mentioning

confidence: 99%

A Mini-review on Ultra Performance Liquid Chromatography

et al. 2021

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“…Considering the lack of strong ultraviolet absorption and relatively low content, liquid chromatography–tandem mass spectrometry (LC-MS) is the preferred method for determining PAs due to its high sensitivity and versatility ( Luo et al, 2019 ; Han et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Pyrrolizidine alkaloids and health risk of three Boraginaceae used in TCM

Zan

Wang²,

Hu³

et al. 2023

Front. Pharmacol.

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Objective: The aim of this study was to systematically explore the pyrrolizidine alkaloids (PAs) type, content and risk assessment in the three Boraginaceae used in TCM, involving Arnebia euchroma (AE), A. guttata (AG), and Lithospermum erythrorhizon (LE).Method: A UHPLC–MS/MS method was established to simultaneously determine eight pyrrolizidine alkaloids (PAs), namely intermedine, lycopsamine, intermedine N-oxide, lycopsamine N-oxide, 7-acetyllycopsamine, 7-acetyllycopsamine N-oxide, echimidine N-oxide, and echimidine in the three herbs. Based on these results, the risk assessment was explored using the routine margin of exposure (MOE) combined with relative potency (REP) for oral and external usage, respectively.Results and Conclusion: Imermedine and imermedine N-oxide were common components in the eight tested PAs. 7-acetyllycopsamine and its N-oxide were not detected in AE; echimidine and its N-oxide were not detected in AG; lycopsamine and its N-oxide, 7-acetyllycopsamine and its N-oxide were not detected in LE. The total contents of 8 PAs in 11 batches of AG was341.56–519.51 μg/g; the content in 15 batches of LE was 71.16–515.73 μg/g, and the content in 11 batches of AE was 23.35–207.13 μg/g. Based on these results, the risk assessment was explored using MOE combined with REP for oral and external usage, respectively. The findings of the risk assessment method of PAs based on MOE combined with the REP factor were consistent with the clinical toxicity results. As an oral herb, AE had low risk or no risk due to its low PA contents, and individual batches of LE were medium risk, while attention should be paid to their clinical use.AG was also low risk. The external use of the three Boraginaceae used in TCM was not associated with any risk. This study systematically explored the PA type and content of the three Boraginaceae used in TCM. Additionally, the refined risk assessment of PAs based on REP provided a more scientific basis for quality evaluation and rational use of the medicinal Boraginaceae used in TCM to improve public health.

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“…On the basis of the molecular and structural features of PAs, analytical techniques with low LOQs are significant in controlling PAs. A variety of analytical techniques have been developed for studying PAs, including CE, HPLC, LC‐MS, and so forth [16–19]. According to the European Food Safety Authority (EFSA) Panel, LC‐MS can be specifically applied for collecting analytical data on food PAs [20].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of eight pyrrolizidine alkaloids in various parts of Eupatorium lindleyanum by ultra high performance liquid chromatography tandem mass spectrometry and risk assessments based on a real‐life exposure scenario

Zan

et al. 2021

J of Separation Science

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Pyrrolizidine alkaloids are toxins having hepatotoxic and carcinogenic effects on human health. A ultra high performance liquid chromatography tandem mass spectrometry technique was developed for the first time for the simultaneous determination of eight pyrrolizidine alkaloids, including four diastereoisomers (intermedine, lycopsamine, rinderine, and echinatine) and their respective N‐oxide forms, in different parts of Eupatorium lindleyanum. The risk assessment method for pyrrolizidine alkaloids in Eupatorium lindleyanum was explored using the margin of exposure strategy for the first time based on a real‐life exposure scenario. Differences were found in all eight pyrrolizidine alkaloids in various parts of Eupatorium lindleyanum. Besides, the total levels of pyrrolizidine alkaloids in Eupatorium lindleyanum followed the order of root > flower > stem > leaf. Moreover, the risk assessment data revealed that the deleterious effects on human health were unlikely at exposure times of less than 200, 37, and 12 days during the lifetimes of Eupatorium lindleyanum leaves, stems, and flowers, respectively. This study reported both the contents of and risk associated with Eupatorium lindleyanum pyrrolizidine alkaloids. The comprehensive application of the novel ultra high performance liquid chromatography tandem mass spectrometry technique alongside the risk assessment approach provided a scientific basis for quality evaluation and rational utilization of toxic pyrrolizidine alkaloids in Eupatorium lindleyanum to improve public health safety.

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Development of UPLC-Q-TOF-MS Coupled with Cation-exchange Solid-phase Extraction Method for the Determination of Ten Pyrrolizidine Alkaloids in Herbal Medicines

Cited by 13 publications

References 42 publications

A Mini-review on Ultra Performance Liquid Chromatography

A Mini-review on Ultra Performance Liquid Chromatography

Pyrrolizidine alkaloids and health risk of three Boraginaceae used in TCM

Simultaneous determination of eight pyrrolizidine alkaloids in various parts of Eupatorium lindleyanum by ultra high performance liquid chromatography tandem mass spectrometry and risk assessments based on a real‐life exposure scenario

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