Development of validated HPLC-UV method for simultaneous determination of Metformin, Amlodipine, Glibenclamide and Atorvastatin in human plasma and application to protein binding studies

Porwal, Pawan K.; Talele, Gokul S.

doi:10.1016/j.bfopcu.2016.10.002

Cited by 20 publications

(17 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glibenclamide is rapidly and completely absorbed from the gastrointestinal tract. Different methods have been used for the determination of glibenclamide among these methods, different HPLC methods coupled with UV detection (Porwal and Talele, 2017) fluorescence detection (Khatri et al, 2001), or mass spectrometry (Ramos et al, 2000).…”

Section: Fig 1: Valsartanmentioning

confidence: 99%

Electrochemical Behavior of Valsartan, Glibenclamide and their Interaction with Each other Using Square Wave Voltammetry

Al-Rawi¹,

Agha²,

Al-Hafidh³

et al. 2019

RJS

View full text Add to dashboard Cite

In this work an electrochemical behavior quantification and interaction of valsartan and glibenclamide were studied using square wave voltammetric technique. The effect of temperature on the interaction was investigated and the thermodynamic parameters (∆H, ∆S & ∆G) were calculated for the interaction and binding constant (K) also obtained. The calibration curves of each drug were linear within the range of concentration [(4.99×10-7)-(6.95×10-6)], [(5.96×10-8)-(1.15×10-6)] molar with R 2 value equal to 0.9819, 0.9926 for valsartan and glibenclamide respectively.

show abstract

Section: Fig 1: Valsartanmentioning

confidence: 99%

Electrochemical Behavior of Valsartan, Glibenclamide and their Interaction with Each other Using Square Wave Voltammetry

Al-Rawi¹,

Agha²,

Al-Hafidh³

et al. 2019

RJS

View full text Add to dashboard Cite

show abstract

“…Analysis of glibenclamide, metformin, captopril, and pioglitazone in API was reported with 70% methanol and 30% water using Hypersil ODS C 18 column [27]. Glibenclamide, amlodipine, atorvastatin, and metformin in human plasma were separated and validated by HPLC-UV method [28]. Antidiabetic and antihypertensive drugs were studied for stability indicating assay using RP HPLC method; the separation was achieved isocratically on a C 18 column [29].…”

Section: Venkataraman and Harithamentioning

confidence: 99%

Assessment of Chromatographic Separations for Antihypertensive and Antidiabetic Drugs

Venkataraman

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Context: The focus of this review is to compile the different chromatographic methods that were reported earlier for the analysis of different antihypertensive and antidiabetic drugs. Objective: The magnitude of chemical entities investigated and entering into the medicinal field for various health-related ailments is escalating year after year. The drugs are either innovative entities or fractional structural variation of the preexisting chemical molecule. These drugs may exhibit unexpected toxicities after Phase IV of clinical trials, resulting in their withdrawal from the market. Under these circumstances, analytical measures for these drugs may not be accessible in the pharmacopeias. The main aim of this work is to compile the different analytical techniques for the quantification of various antihypertensive drugs and antidiabetic drugs. Methods: The present work is to thoroughly study the literature for the application of different analytical techniques such as high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy/tandem mass spectroscopy (LC-MS/MS) for the quantification of antihypertensive drugs and anti-diabetic drugs. Results: The present study attempts to collate various analytical techniques that were developed and validated for the estimation of few important antidiabetic and antihypertensive drugs either in pure, individually or combined with other pharmaceutical dosage form by HPLC, LC-MS/MS, and high-performance thin-layer chromatography techniques. Conclusion: Different chromatographic methods are considered to be rapid tools for qualitative and quantitative analysis of newer chemical entities in pharmaceuticals. The amount of these newer chemical entities which are reaching the pharmaceutical market is increasing day by day nevertheless there exists a lag in establishing the standard protocols for the identification, impurity profiling, related substance and assay method. Hence, the present review compiles the different analytical methods that were reported in the literature and thus helps the researchers and chemists to make use of the analytical techniques for the quantification and validation of various antidiabetic and antihypertensive drugs.

show abstract

“…The concept of combining agents with manifestly different indications has become a certainty of treatment in patients with a chronic disease where it requires treatment for several different pharmacotherapies (Porwal and Talele, 2017). As a primary treatment to diabetic hypertensive subjects, generally, a ixed-dose of GLB and ATN or combination of drugs is commonly prescribed (Sengupta et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a new analytical RP-HPLC method for simultaneous determination of Glibenclamide and Atenolol in bulk

Anitha¹,

Ramkanth²,

Satyanarayana³

2019

ijrps

View full text Add to dashboard Cite

A new, simple, reliable, fast, sensitive and economical RP-HPLC method was developed and validated for simultaneous estimation of two fixed-dose combinations frequently prescribed in coexisted chronic diseases such as diabetes (GLB) and hypertension (ATN) in bulk for the first time. The mobile phase used for the chromatographic runs consisted of 0.01N potassium dihydrogen ortho phosphate (pH 4.8) and acetonitrile (55:45, v/v). The separation was achieved on column (BDS C18 250 x 2.1mm, 1.6m) using isocratic mode. Drug peaks were well separated and were detected by a UV detector at 235.0 nm. The method was linear at the concentration range 2.5-15µg/ml for Glibenclamide (GLB) and 6.25-37.5µg/ml for Atenolol (ATN), respectively. The method has been validated according to ICH guidelines with respect to system suitability, specificity, precision, accuracy and robustness. The method was validated for system suitability, linearity, accuracy, precision, detection, quantification limits and robustness and was found it is acceptable in the range of 2.5–15 µg/ml for GLB and 6.25–37.5 µg/ml for ATN. The LOD and LOQ of GLB was found to be 0.48 µg/ml and 1.47µg/ml and for ATN was found to be 0.72µg/ml and 2.20 µg/ml, respectively. The method was applied to drug interaction studies of GLB with ATN to illustrate the scope and application of the methods to manage two different therapeutic classes of drugs, as they may co-administered in concurrent diseases.

show abstract

Development of validated HPLC-UV method for simultaneous determination of Metformin, Amlodipine, Glibenclamide and Atorvastatin in human plasma and application to protein binding studies

Cited by 20 publications

References 32 publications

Electrochemical Behavior of Valsartan, Glibenclamide and their Interaction with Each other Using Square Wave Voltammetry

Electrochemical Behavior of Valsartan, Glibenclamide and their Interaction with Each other Using Square Wave Voltammetry

Assessment of Chromatographic Separations for Antihypertensive and Antidiabetic Drugs

Development and validation of a new analytical RP-HPLC method for simultaneous determination of Glibenclamide and Atenolol in bulk

Contact Info

Product

Resources

About