Development of yellow‐seeded Brassica napus of double low quality

Abstract

Two yellow‐seeded white‐petalled Brassica napus F7 inbred lines, developed from interspecific crosses, containing 26–28% emcic acid and more than 40 μmol glucosinolates (GLS)/g seed were crossed with two black/dark brown seeded B. napus varieties of double low quality and 287 doubled haploid (DH) lines were produced. The segregation in the DH lines indicated that three to four gene loci are involved in the determination of seed colour, and yellow seeds are formed when all alleles in all loci are in the homozygous recessive state. A dominant gene governed white petal colour and is linked with an erucic acid allele that, in the homozygous condition, produces 26–28% erucic acid. Four gene loci are involved in the control of total GLS content where low GLS was due to the presence of recessive alleles in the homozygous condition in all loci. From the DH breeding population a yellow‐seeded, yellow‐petalled, zero erucic acid line was obtained. This line was further crossed with conventional B. napus varieties of double low quality and, following pedigree selection, a yellow seeded B. napus of double low quality was obtained. The yellow seeds had higher oil plus protein content and lower fibre content than black seeds. A reduction of the concentration of chromogenic substances was found in the transparent seed coat of the yellow‐seeded B. napus.

Proanthocyanidin biosynthesis in the seed coat of yellow-seeded, canola qualityBrassica napusYN01-429 is constrained at the committed step catalyzed by dihydroflavonol 4-reductaseThis paper is one of a selection of papers published in a Special Issue from the National Research Council of Canada – Plant Biotechnology Institute.

“…There are many ongoing efforts to breed a yellow-seeded B. napus that does not have dark pigments in the seed coat (Tang et al 1997;Badani et al 2006;Rahman 2001;Rahman et al 2001). Such seeds are yellow because the underlying embryo is visible through a translucent seed coat.…”

Identification of QTL for seed coat colour and oil content inBrassica napusby association mapping using SSR markers

“…Since seed oil content is positively correlated with seed coat colour, the quality of rapeseed crops could be improved through the development of yellow-seeded cultivars (Rahman 2001). However, seed coat colour and seed oil content are typical quantitative traits under polygenic control in B. napus, mainly being determined by polygene (Shirzadegan 1986;Rahman et al 2001;Zhao 2002;Liu et al 2005a;Badani et al 2006) and influenced by factors such as maternal effects and the environment (Van Deynze et al 1993;Zhao et al 2005). Therefore, detecting the loci that control these complex traits by quantitative trait locus (QTL) mapping will pave the way for markerassisted selection.…”

A new yellow-seeded canola genotype originating from double low black-seeded Brassica napus cultivars

“…Although the gene control and origin of the yellow colour in these lines is currently under investigation, it is expected that the seed colour may be controlled by fewer genes than those previously described in the literature for yellow-seeded types derived from interspecific crosses and based on a specific combination of multiple recessive genes (Henderson and Pauls 1991; Rahman et al 2001). In this study out of 26 doubled haploids produced from the original Star × Bolero F 1 , 6 (20:6) carried the yellow-seeded phenotype.…”

“…Recessive genes at multiple loci are involved in a complex inheritance of the yellow seed trait in these genotypes (Henderson and Pauls 1992). Due to extensive outcrossing to non-canola quality yellow-seeded related species, these derived lines initially carry a multitude of adverse agronomic and oil quality traits that must be eliminated by numerous cycles of crossing and selection, to recapture canola quality in the new yellow-seeded lines (Rakow et al 1999a;Rahman et al 2001;Relf-Eckstein et al 2003).…”