Development of γ-Glutamyltranspeptidase Activity in Mouse Small Intestine: Influence of Cortisone and Thyroxine

Ménard, Daniel; Malo, Christiane; Calvert, R.

doi:10.1159/000241474

Cited by 18 publications

(6 citation statements)

References 9 publications

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“…The level of activity in the fetal mouse liver reaches 1% of the level in the adult kidney (33). GGT is also present transiently during the development of other tissues (34)(35)(36)(37).…”

Section: Localization Of Ggtmentioning

confidence: 99%

Gamma-glutamyl transpeptidase – its role in hepatocarcinogenesis

1985

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“…The level of activity in the fetal mouse liver reaches 1% of the level in the adult kidney (33). GGT is also present transiently during the development of other tissues (34)(35)(36)(37).…”

Section: Localization Of Ggtmentioning

confidence: 99%

Gamma-glutamyl transpeptidase – its role in hepatocarcinogenesis

1985

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“…The differentiation of absorptive cell is char acterized at the enzymatic level, by the ap pearance of sucrase activity at 15 days of age. Furthermore, trehalase, maltase, glucoamylase, alkaline phosphatase and peptidase ac tivities increase while lactase activity de clines to the adult value [ 11,17,19,21], Dur ing the same period, the mitotic index, the labelling index and the cellular turnover in crease while the cell cycle becomes shorter [7,10,28).…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of Insulin and Thyroxine on the Differentiation and Proliferation of Epithelial Cells of Suckling Mouse Small Intestine

Malo¹,

Ménard²

1983

Neonatology

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The interactions between insulin, cortisone and thyroxine on the functional maturation of the small intestine were investigated. 8-day-old suckling mice received one injection/day during 3 days of a single hormone or of the following hormonal combinations: insulin plus cortisone or insulin plus thyroxine. The differentiation of intestinal epithelial cells was evaluated by measuring brush border membrane hydrolytic activities and proliferation by establishing the mitotic index and ³H-thymidine incorporation into DNA. The effects of cortisone and insulin on enterocyte’s differentiation are additive. Thyroxine accelerates the differentiation and has a limited action on cell proliferation. Insulin exerts a sustained effect on these two aspects of the intestinal maturation and, furthermore, acts in synergism with thyroxine. It appears that insulin, cortisone and thyroxine have their own specific effect on differentiation and proliferation of the epithelial cells and that these three hormones act in cooperation to induce the maturation of the suckling mouse small intestine during postnatal development.

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“…The importance of hydrocortisone (HC) and thyroxine as factors inducing precocious maturation of the intestinal epithelium has been well documented (Deren, 1968;Henning and 6; 1991 WILEY-LISS, INC. Kretchmer, 1973;Moog, 1981). The influence of insulin (Menard et al, 1981;Moog and Goellner, 1982) and epidermal growth factor (EGF) (Malo and Menard, 1982) has been demonstrated, and synergy between insulin and thyroxine has been reported (Malo and Menard, 1983). Most of these studies were performed in vivo, an approach which allows the identification of factors involved in the regulation of enterocyte differentiation but which cannot provide information on the mechanisms underlying their effects.…”

mentioning

confidence: 99%

Caco‐2 cells cultured in serum‐free medium as a model for the study of enterocytic differentiation in vitro

Jumarie

Malo

1991

Journal Cellular Physiology

195

149

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Caco-2 cells, which express spontaneous enterocytic differentiation at confluency, is one of the most relevant in vitro models for the study of differentiation and regulation of intestinal functions. However, these cells are normally cultured in the presence of 15-20% serum which renders extremely complex the identification of the factors involved in the regulation of both proliferation and differentiation. This study has been devoted to the establishment of chemically defined culture conditions which can sustain growth and differentiation of Caco-2 cells. The replacement of serum by ITS (insulin, transferrin, and selenium) allowed for normal structural and functional differentiation of cells as revealed by the establishment of cell polarity and the expression of brush-border membrane enzyme markers (sucrase, maltase, lactase, alkaline phosphatase, gamma-glutamyltransferase, aminopeptidase N, and dipeptidyl-dipeptidase IV), although the levels of sucrase activity were lower in ITS-supplemented medium. Coating petridishes with either type IV collagen or basement membrane proteins (Matrigel) did not improve the differentiation of cells, brush-border membrane enzyme activities being, in fact, lower when the cells were grown on these substrata. When triiodothyronine (T3, 5 x 10(-8) M) was added to the ITS-supplemented medium, disaccharidase and alkaline phosphatase activities were significantly increased while gamma-glutamyltransferase activity was diminished by T3 and stimulated by epidermal growth factor (1.6 x 10(-6) M). On the other hand, hydrocortisone (HC, 10(-6) M) did not modify disaccharidase and peptidase activities. These data clearly show that Caco-2 cells can be maintained in serum-free medium and that this system allows the study of the factors involved in the regulation of the differentiation of enterocyte in vitro.

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Development of γ-Glutamyltranspeptidase Activity in Mouse Small Intestine: Influence of Cortisone and Thyroxine

Cited by 18 publications

References 9 publications

Gamma-glutamyl transpeptidase – its role in hepatocarcinogenesis

Gamma-glutamyl transpeptidase – its role in hepatocarcinogenesis

Synergistic Effects of Insulin and Thyroxine on the Differentiation and Proliferation of Epithelial Cells of Suckling Mouse Small Intestine

Caco‐2 cells cultured in serum‐free medium as a model for the study of enterocytic differentiation in vitro

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