Development, physicochemical characterization and preclinical efficacy evaluation of a water soluble glucan sulfate derived from Saccharomyces cerevisiae

Williams, David L.; Pretus, Henry A.; McNamee, Rose B.; Jones, Ernest L.; Ensley, Harry E.; Browder, I. William; Luzio, N. R. Di

doi:10.1016/0162-3109(91)90039-2

Cited by 69 publications

(22 citation statements)

References 44 publications

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“…9 -10 Results from in vitro and in vivo experiments suggest that glucans can bind to cells by receptors, stimulate bone marrow, activate macrophages and induce production of cytokines such as interleukin (IL)-1 and IL-2. 9, [11][12][13][14] In vitro studies show that grifolan, a glucan isolated from Grifola frondosa, stimulates the secretion of inflammatory cytokines such as tumour necrosis factor-a (TNF-a ) and IL-6 from cultured macrophages. 14 The effects after inhalation are less well known.…”

Section: Introductionmentioning

confidence: 99%

Effects after inhalation of (1→3)-β-D-glucan in healthy humans

Thornca¹,

Beijer²,

Rylander³

2001

Mediators Inflamm.

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Background and aim: This study was performed to assess the effects of an exposure to a pure (1 ® 3)-b -D-glucan, a cell wall component of fungi, plants and certain bacteria.Methods: Twenty-one healthy subjects inhaled saline or (1 ® 3)-b -D-glucan suspended in saline in a random, double-blind, cross-over design. They were examined before exposure and 24 and 72 h afterwards with spirometry, blood sampling and collection of induced sputum. Differential cell counts and eosinophilic cationic protein (ECP) were determined in blood and sputum, and myeloperoxidase (MPO), tumour necrosis factor-a (TNF-a ), and interleukin (IL)-8 and IL-10 were determined in sputum supernatants. TNF-a was determined after cultivation of blood mononuclear cells. Results: In sputum, inhalation of saline caused a significant increase in ECP and TNF-a . (1 ® 3)-b -D-Glucan inhalation caused a further increase in these cytokines, although not statistically significantly different from the increase induced by inhalation of saline alone. In blood, the number of eosinophils was significantly decreased 72 h after the challenge with (1 ® 3)-b -D-glucan. This effect was not found after the inhalation of saline alone. TNF-a production from stimulated blood mononuclear cells was significantly decreased 72 h after the (1 ® 3)-b -D-glucan inhalation as compared with the increase induced by saline inhalation. Conclusions: The results suggest that (1 ® 3)-b -D-glucancauses a different type of response as compared with inflammatory agents such as bacterial endotoxin that cause a neutrophil-dominated inflammatory response.

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Section: Introductionmentioning

confidence: 99%

Effects after inhalation of (1→3)-β-D-glucan in healthy humans

Thornca¹,

Beijer²,

Rylander³

2001

Mediators Inflamm.

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“…It was reported that sulfated polysaccharides might lead to antitumor activity [3,7,20]. In the present study, the growth inhibitory effects of POP1 and the five sulfated derivatives against HepG2 cells in vitro were first examined in Table 2.…”

Section: Growth Inhibition Of Sulfated Derivatives On Hepg2 Cellsmentioning

confidence: 99%

Sulfated modification and cytotoxicity of Portulaca oleracea L. polysaccharides

Chen

Jin

et al. 2010

Glycoconj J

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A water-soluble polysaccharide (POP1) was isolated from Portulaca oleracea L. Four sulfated derivatives of POP1 (POP1-s1, POP1-s2, POP1-s3 and POP1-s4) were prepared by chlorosulfonic acid method with N,N-Dicyclohexylcarbodiimide (DCC) as a dehydration-condensation agent. FT-IR spectra and 13C NMR spectra indicated the sulfated groups had been introduced at the C-6 and C-2 positions of POP1. Sulfated derivatives had different degree of substitution (DS) ranging from 1.01 to 1.81, and different weight-average molecular mass (Mw) ranging from 41.4 to 48.5 KDa. Sulfated derivatives except POP1-s5 inhibited the growth of HepG2 cells and Hela cells in vitro significantly, which indicated that sulfated modification could enhance cytotoxicity of POP1 on tumor cells. Flow cytometric studies revealed that sulfated derivatives could mediate the cell-cycle arrest of Hela cells in the S phase.

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“…b-Glucans are the glucose polymers derived from the cell wall of baker's yeast (Saccharomyces cerevisiae), fungi, and cereal plants [8][9][10]. Several mechanisms have been proposed for the protective effect of b-glucan molecule.…”

Section: Introductionmentioning

confidence: 99%