Development, validation and testing of a human tissue engineered hypertrophic scar model

Broek, L. J. van den; Niessen, Frank B.; Scheper, Rik J.; Gibbs, Susan

doi:10.14573/altex.2012.4.389

Cited by 54 publications

(38 citation statements)

References 43 publications

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“…This leads to a model in which ADSCs are responsible for hypertrophic scar formation. In a tissue-engineered human scar model, ADSCs isolated from healthy human skin (in contrast to dermal mesenchymal cells) indeed facilitate the hypertrophic scar formation [25]. …”

Section: The Versatile Dermal Adipocyte: Immature and Mature Adipocytmentioning

confidence: 99%

Dermal Adipocytes: From Irrelevance to Metabolic Targets?

Kruglikov¹,

Scherer

2016

Trends in Endocrinology & Metabolism

106

105

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Dermal white adipose tissue (dWAT) has found little appreciation in the past as a distinct entity from the better recognized subcutaneous white adipose tissue (sWAT). However, recent work has established dWAT as an important contributor to a multitude of processes, including immune response, wound healing and scarring, hair follicle growth and thermoregulation. Unique metabolic contributions are attributed to dWAT as well, at least in part due to thermic insulation properties and its response to cold exposure. Dermal adipocytes can also undergo adipocyte-myofibroblast transition (AMT), a process that is suspected to play an important role in a number of pathophysiological processes within the skin. Here, we discuss emerging concepts regarding dWAT physiology and its significance to a variety of cellular processes.

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Section: The Versatile Dermal Adipocyte: Immature and Mature Adipocytmentioning

confidence: 99%

Dermal Adipocytes: From Irrelevance to Metabolic Targets?

Kruglikov¹,

Scherer

2016

Trends in Endocrinology & Metabolism

106

105

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“…1(b); pig ear skin tissue is extremely similar to that of human tissue. 12,13 The thicknesses of the stratum corneum and living epidermis in the pig ear were approximately 20 and 100 m, respectively. Similar to human skin, we determined that blood capillaries in the dermis layer of the pig ear opened and began to bleed at a perforation depth of 120-220 m.…”

Section: Methodsmentioning

confidence: 99%

Accurate laser skin perforation technique aimed at promoting bleeding and reducing pain

Chang

Lin

Huang

2015

J. Innov. Opt. Health Sci.

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Laser skin perforation is an e®ective and promising technique for use in blood collection. In this study, the relation between the perforation pro¯le of skin and laser irradiation at various energies is discussed. Increasing laser energy does not uniformly expand the size and depth of a hole because the shallow depth of¯eld (DOF) of the focused light primarily concentrates energy on the skin surface. In practice, the hole gradually transforms from a semielliptical shape to an upsidedown avocado shape as the laser energy increases. This phenomenon can increase the amount of bleeding and reduce pain. The¯ndings support the feasibility of developing an accurate laser skin perforation method.

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“…Hypertrophic scars were induced on the ventral surface of rabbit ears as previously described [11,12]. Briefly, the rabbits were anesthetized with ketamine (45 mg/kg), and then cycloid wounds 1 cm in diameter were created down to bare cartilage and dislodged the perichondrium on the rabbit's ear, four wounds in each ear.…”

Section: Methodsmentioning

confidence: 99%

Compound Astragalus and Salvia miltiorrhiza Extract Suppresses Rabbits' Hypertrophic Scar by Modulating the TGF-β/Smad Signal

Jiang²,

Boye³

et al. 2014

Dermatology

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Background: Hypertrophic scar is a fibro-proliferative disease. Our previous studies demonstrate that compound Astragalus and Salvia miltiorrhiza extract (CASE) inhibits proliferation and invasion in keloid fibroblasts. Objective: To investigate the effects of CASE on hypertrophic scar. Methods: Rabbits were divided into the control, model and three dosage groups of CASE (0.94, 1.88, 3.76%). An animal model of hypertrophic scar was established and treated with CASE ointment or ointment base. The histopathological detection by hematoxylin & eosin and Masson's trichrome staining and protein expression of scars by Western blot were performed. Results: The hydroxyproline content was decreased under CASE treatment. Transforming growth factor beta 1 (TGF-β₁) protein expression increased in the model group while it decreased under CASE treatment. The elevated expression of Smad4 protein was decreased under CASE treatment. Additionally, CASE promoted Smad7 protein expression. Conclusion: CASE could inhibit formation of hypertrophic scar by modulating TGF-β/Smad signal and may be useful for the treatment of hyperplastic scars.

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Development, validation and testing of a human tissue engineered hypertrophic scar model

Abstract: Summary

Cited by 54 publications

References 43 publications

Dermal Adipocytes: From Irrelevance to Metabolic Targets?

Dermal Adipocytes: From Irrelevance to Metabolic Targets?

Accurate laser skin perforation technique aimed at promoting bleeding and reducing pain

Compound <b><i>Astragalus</i></b> and <b><i>Salvia miltiorrhiza</i></b> Extract Suppresses Rabbits' Hypertrophic Scar by Modulating the TGF-β/Smad Signal

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