Developmental age strengthens barriers to ethanol accumulation in zebrafish

Lovely, C. Ben; Nobles, Regina D.; Eberhart, Johann K.

doi:10.1016/j.alcohol.2014.06.003

Cited by 44 publications

(58 citation statements)

References 44 publications

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“…The present work solidifies a growing consensus that the zebrafish embryo contains approximately 24–37% of media ethanol concentrations (Flentke et al, 2014; Lovely et al, in review; Reimers et al, 2004; Zhang et al, 2013). While these studies use differing assays for reporting ethanol concentration, there is similarity in experimental design allowing for comparison.…”

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confidence: 79%

Commentary: Catching a Conserved Mechanism of Ethanol Teratogenicity

Lovely

Eberhart

2014

Alcoholism Clin & Exp Res

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Due to its profound impact on human development, ethanol teratogenicity is a field of intense study. The complexity of variables that influence the outcomes of embryonic or prenatal ethanol exposure compels the use of animal models in which these variables can be isolated. Numerous model systems have been used in these studies. The zebrafish is a powerful model system, which has seen a recent increase in usage for ethanol studies. Those using zebrafish for alcohol studies often face two questions: 1) How physiologically relevant are the doses of ethanol administered to zebrafish embryos and 2) Will the mechanisms of ethanol teratogenesis be conserved to other model systems and human? The current manuscript by Flentke et al helps to shed important light on these questions and clearly demonstrates that the zebrafish will be a valuable model system with which to understand ethanol teratogenicity.

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supporting

confidence: 79%

Commentary: Catching a Conserved Mechanism of Ethanol Teratogenicity

Lovely

Eberhart

2014

Alcoholism Clin & Exp Res

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“…Genetic background (AB, Tu, or fli1:EGFP) did not appear to have a substantial impact on the tissue levels of ethanol. 29 Specifically, consistent but nonsignificant effects were observed with AB appearing to accumulate ethanol to a lower extent than the other backgrounds. 29 Similarly, the chorion does not appear to be a major barrier to ethanol.…”

Section: Models Of Fasd: Return Of the Fishmentioning

confidence: 71%

“…29 Specifically, consistent but nonsignificant effects were observed with AB appearing to accumulate ethanol to a lower extent than the other backgrounds. 29 Similarly, the chorion does not appear to be a major barrier to ethanol. 28,29,32 However, the developing embryo can show varying ethanol sensitivities based on genetic backgrounds (Ekkwill, AB, or Tu).…”

Section: Models Of Fasd: Return Of the Fishmentioning

confidence: 71%

“…29 Similarly, the chorion does not appear to be a major barrier to ethanol. 28,29,32 However, the developing embryo can show varying ethanol sensitivities based on genetic backgrounds (Ekkwill, AB, or Tu). 33 Interestingly, the tissues most affected by ethanol exposure varied among the different backgrounds with Tu having the lowest survival rate, but being the most resistant to craniofacial malformations.…”

Section: Models Of Fasd: Return Of the Fishmentioning

confidence: 99%

“…28 Our group used Headspace Gas Chromatography and found tissue levels to be *24%-37%, depending upon the age of the embryo, with older embryos having lower tissue levels. 29 One possible reason for the early disagreement in tissue levels of ethanol may be the very rapid rate at which ethanol levels equilibrate, [28][29][30][31] and therefore, extended or multiple washes would result in an underestimation of the ethanol concentration. Collectively, these studies indicate that the use of ethanol concentrations at or above 2% in the media is likely to result in suprapharmacological levels of ethanol.…”

Section: Models Of Fasd: Return Of the Fishmentioning

confidence: 99%

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Fishing for Fetal Alcohol Spectrum Disorders: Zebrafish as a Model for Ethanol Teratogenesis

2016

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Fetal Alcohol Spectrum Disorders (FASD) describes a wide array of ethanol-induced developmental defects, including craniofacial dysmorphology and cognitive impairments. It affects *1 in 100 children born in the United States each year. Due to the pleiotropic effects of ethanol, animal models have proven critical in characterizing the mechanisms of ethanol teratogenesis. In this review, we focus on the utility of zebrafish in characterizing ethanolinduced developmental defects. A growing number of laboratories have focused on using zebrafish to examine ethanol-induced defects in craniofacial, cardiac, ocular, and neural development, as well as cognitive and behavioral impairments. Growing evidence supports that genetic predisposition plays a role in these ethanol-induced defects, yet little is understood about these gene-ethanol interactions. With a high degree of genetic amenability, zebrafish is at the forefront of identifying and characterizing the gene-ethanol interactions that underlie FASD.

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Differentially sensitive neuronal subpopulations in the central nervous system and the formation of hindbrain heterotopias in ethanol‐exposed zebrafish

Buckley

Sidik

Kar

et al. 2019

Birth Defects Research

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Background A cardinal feature of prenatal ethanol exposure is CNS damage, resulting in a continuum of neurological and behavioral impairments that are described by the term Fetal Alcohol Spectrum Disorders (FASD). FASDs are variable and depend on several factors, including the amount, timing and duration of prenatal ethanol exposure. To enhance interventions for CNS dysfunction, it is necessary to identify ethanol-sensitive neuronal populations and expand the understanding of factors that modify ethanol teratogenesis. Methods: To investigate the susceptibility of different neuronal subtypes, we exposed transgenic zebrafish (Danio rerio) to several ethanol concentrations (0.25%, 0.5%, 1.0%, 1.5% or 2.0%), at different hours post fertilization (hpf) (0 hpf, 6 hpf or 24 hpf), for various durations (0–24hpf, 0–48hpf, 4–24hpf, 6–24hpf, 6–48hpf or 24–48hpf). Following exposure, embryo survival rates were determined, and CNS neurogenesis, differentiation and patterning was assessed. Results: Embryo survival rates decrease as ethanol concentrations increase and drastically decline when exposed from 0–24hpf compared to 4–24hpf. Abnormal tangential migration of facial motor neurons is observed in isl1:gfp embryos exposed to ethanol concentrations as low as 0.25%, and the formation of IVth ventricle heterotopias are revealed by embryos exposed to ≥1.0% ethanol. Whereas, expression of olig2:dsred and ptf1a:gfp in the cerebellum and spinal cord are largely unaffected. While levels of etv4 mRNA are overtly resistant to ethanol, we observe significant reductions in ptch2 mRNA levels. Conclusions: These data show differentially sensitive CNS neuron subpopulations with susceptibility to low levels of ethanol. In addition, these data reveal the formation of ethanol-induced hindbrain heterotopias.

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Developmental age strengthens barriers to ethanol accumulation in zebrafish

Cited by 44 publications

References 44 publications

Commentary: Catching a Conserved Mechanism of Ethanol Teratogenicity

Commentary: Catching a Conserved Mechanism of Ethanol Teratogenicity

Fishing for Fetal Alcohol Spectrum Disorders: Zebrafish as a Model for Ethanol Teratogenesis

Differentially sensitive neuronal subpopulations in the central nervous system and the formation of hindbrain heterotopias in ethanol‐exposed zebrafish

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