Developmental Assembly of the Tight Junction

Fleming, Tom P.; Sheth, Bhavwanti; Thomas, Fay; Fesenko, Irina; Eckert, Judith

doi:10.1201/9781420038538.ch13

Cited by 4 publications

(6 citation statements)

References 104 publications

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“…Occludin and ZO-1␣ ϩ are assembled in the last stage (32 cells) (408,410). In mouse and human, the TJ mRNAs of claudin-1, JAM, occludin TM4 ϩ and TM4, and ZO-1␣ Ϫ are initially inherited from maternal transcription and followed by embryonic transcription from the two-cell stage onward, and remain present throughout preimplantation development (154,155,176). Only ZO-1␣ ϩ , ZO-2, and desmocolin-2, a component of desmosomes, are transcribed later on from the embryonic genome, during 16-to 32-cell stage in human and mouse (176,408).…”

Section: H Biogenesismentioning

confidence: 99%

Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans

Cereijido

Contreras

Shoshani

2004

Physiological Reviews

151

100

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Transporting epithelia posed formidable conundrums right from the moment that Du Bois Raymond discovered their asymmetric behavior, a century and a half ago. It took a century and a half to start unraveling the mechanisms of occluding junctions and polarity, but we now face another puzzle: lest its cells died in minutes, the first high metazoa (i.e., higher than a sponge) needed a transporting epithelium, but a transporting epithelium is an incredibly improbable combination of occluding junctions and cell polarity. How could these coincide in the same individual organism and within minutes? We review occluding junctions (tight and septate) as well as the polarized distribution of Na+-K+-ATPase both at the molecular and the cell level. Junctions and polarity depend on hosts of molecular species and cellular processes, which are briefly reviewed whenever they are suspected to have played a role in the dawn of epithelia and metazoan. We come to the conclusion that most of the molecules needed were already present in early protozoan and discuss a few plausible alternatives to solve the riddle described above.

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Section: H Biogenesismentioning

confidence: 99%

Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans

Cereijido

Contreras

Shoshani

2004

Physiological Reviews

151

100

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“…Most TJ mRNAs in cattle embryos followed the most common maternal and embryonic transcription pattern [26], which is similar to that of the mouse [3,11]. This expression pattern was not affected by in vitro conditions for four transcripts, namely, pan ZO-1, ZO-1␣ϩ, pan ZO-2, and ZO-2␤ϩ.…”

Section: Discussionmentioning

confidence: 73%

“…1 This work was supported by grant B104-CT98-0032 from the European Union. 2 Correspondence: Judith J. Eckert, Division of Cell Sciences, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton SO16 7PX, United Kingdom; e-mail: jje@soton.ac.uk 3 These two authors contributed equally to this study. These are belt-like, multiprotein complexes around each cell that control intercellular sealing so that vectorial transport across the TE will result in expansion of the blastocelic cavity.…”

Section: Introductionmentioning

confidence: 98%

“…These are belt-like, multiprotein complexes around each cell that control intercellular sealing so that vectorial transport across the TE will result in expansion of the blastocelic cavity. The TJ consists of integral membrane proteins (occludin, claudins, junction adhesion molecule [JAM]) that engage in intercelluar sealing, and a number of cytoplasmic plaque proteins (including ZO-1 and ZO-2) that both link the membrane constituents to the actin cytoskeleton and/or participate in signaling events that coordinate assembly and functioning of the TJ seal [1][2][3]. Several TJ proteins also exist as different isoforms generated by alternative splicing.…”

Section: Introductionmentioning

confidence: 99%

“…The ZO-2 plaque protein also assembles during the early 8-to 16-cell stage (Nowak et al, unpublished observations). However, most TJ mRNAs are present throughout preimplantation development in the mouse, initially inherited from maternal transcription followed by embryonic transcription from the 2-cell stage onward [3,11]. Only ZO-1␣ϩ is transcribed later from the embryonic genome (16-to 32-cell stage in the mouse [6]).…”

Section: Introductionmentioning

confidence: 99%

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Tight Junction Messenger RNA Expression Levels in Bovine Embryos are Dependent upon the Ability to Compact and In Vitro Culture Methods1

Miller¹,

Eckert²,

Lazzari³

et al. 2003

Biology of Reproduction

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We have established a transcription map of individual bovine embryos using semiquantitative reverse transcriptase-polymerase chain reaction to detect the levels of six marker genes involved in early embryo differentiation. The critical step of compaction during preimplantation development is often not accomplished or it takes place for only a short period in in vitro generated embryos, which may result in reduced viability. Compaction is accompanied by the assembly of intercellular tight junctions (TJs) as a barrier against the extraembryonic environment and as a prerequisite for blastocele formation. In the present study, we have related the expression of TJ gene mRNA in individual bovine embryos to their developmental stage, their competence to undergo a clear period of compaction before blastocyst formation, and their in vitro or in vivo origin. Our results indicate that embryos that showed a detectable and well-formed compaction period in vitro are of similar quality to their in vivo counterparts. Starting from the same amount of maternal message, in vivo and in vitro development differ most during the critical period of the major switch from maternal to embryonic genomic control before a dramatic increase of TJ mRNAs occurs upon blastocyst formation. Failure to compact in vitro results in significant reduction of specific transcript levels, in a manner that depends on culture conditions, which may contribute to reduced viability. We conclude that TJ mRNA expression levels are sensitive to environmental conditions that may influence the developmental potential of bovine blastocysts.

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Tight junction proteins

González‐Mariscal

Betanzos

Nava

et al. 2003

Progress in Biophysics and Molecular Biology

1,005

589

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A fundamental function of epithelia and endothelia is to separate different compartments within the organism and to regulate the exchange of substances between them. The tight junction (TJ) constitutes the barrier both to the passage of ions and molecules through the paracellular pathway and to the movement of proteins and lipids between the apical and the basolateral domains of the plasma membrane. In recent years more than 40 different proteins have been discovered to be located at the TJs of epithelia, endothelia and myelinated cells. This unprecedented expansion of information has changed our view of TJs from merely a paracellular barrier to a complex structure involved in signaling cascades that control cell growth and differentiation. Both cortical and transmembrane proteins integrate TJs. Among the former are scaffolding proteins containing PDZ domains, tumor suppressors, transcription factors and proteins involved in vesicle transport. To date two components of the TJ filaments have been identified: occludin and claudin. The latter is a protein family with more than 20 members. Both occludin and claudins are integral proteins capable of interacting adhesively with complementary molecules on adjacent cells and of co-polymerizing laterally. These advancements in the knowledge of the molecular structure of TJ support previous physiological models that exhibited TJ as dynamic structures that present distinct permeability and morphological characteristics in different tissues and in response to changing natural, pathological or experimental conditions.

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Developmental Assembly of the Tight Junction

Cited by 4 publications

References 104 publications

Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans

Cell Adhesion, Polarity, and Epithelia in the Dawn of Metazoans

Tight Junction Messenger RNA Expression Levels in Bovine Embryos are Dependent upon the Ability to Compact and In Vitro Culture Methods1

Tight junction proteins

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