Developmental Block and Programmed Cell Death in Bos indicus Embryos: Effects of Protein Supplementation Source and Developmental Kinetics

Garcia, Sheila Merlo; Marinho, Luciana Simões Rafagnin; Lunardelli, Paula Alvares; Seneda, Marcelo Marcondes; Meirelles, Flávio Vieira

doi:10.1371/journal.pone.0119463

Cited by 22 publications

(13 citation statements)

References 38 publications

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“…Recent gene expression studies show that transcripts for select BMP ligands (BMP2, 3, 7, 10), type I and type II BMP receptors and their associated SMAD signaling proteins are elevated during the pre-compaction period but reduced after embryonic genome activation at 8–16 cell stage in cattle [15, 21]. In contrast, TGF-beta ligands such as BMP4 and BMP15 are prominently expressed during the post compaction periods (after embryonic genome activation) for both in vivo and in vitro produced bovine embryos [22].…”

Section: Discussionmentioning

confidence: 99%

Pre- and Peri-/Post-Compaction Follistatin Treatment Increases In Vitro Production of Cattle Embryos

et al. 2017

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Our previous studies demonstrated that maternal (oocyte derived) follistatin (FST) expression is positively associated with bovine oocyte competence and exogenous follistatin treatment during the pre-compaction period of development (d 1–3 post insemination) is stimulatory to bovine early embryogenesis in vitro [blastocyst rates and cell numbers/allocation to trophectoderm (TE)]. In the present study, bovine embryos were treated with exogenous follistatin during d 1–3, d 4–7 and d 1–7 post insemination to test the hypothesis that embryotropic effects of exogenous follistatin are specific to the pre-compaction period (d 1–3) of early embryogenesis. Follistatin treatment during d 4–7 (peri-/post-compaction period) of embryo culture increased proportion of embryos reaching blastocyst and expanded blastocyst stage and total cell numbers compared to controls, but blastocyst rates and total cell numbers were lower than observed following d 1–3 (pre-compaction) follistatin treatment. Follistatin supplementation during d 1–7 of embryo culture increased development to blastocyst and expanded blastocyst stages and blastocyst total cell numbers compared to d 1–3 and d 4–7 follistatin treatment and untreated controls. A similar increase in blastocyst CDX2 mRNA and protein (TE cell marker) was observed in response to d 1–3, d 4–7 and d 1–7 follistatin treatment. However, an elevation in blastocyst BMP4 protein (TE cell regulator) was observed in response to d 1–3 and d 1–7, but not d 4–7 (peri-/post-compaction) follistatin treatment. In summary, our study revealed the potential utility of follistatin treatment for increasing the success rate of in vitro embryo production in cattle. Such results also expand our understanding of the embryotropic actions of follistatin and demonstrate that follistatin actions on blastocyst development and cell allocation to the TE layer are not specific to the pre-compaction period.

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Section: Discussionmentioning

confidence: 99%

Pre- and Peri-/Post-Compaction Follistatin Treatment Increases In Vitro Production of Cattle Embryos

et al. 2017

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“…Research has found that the expression of MCP2 and its receptors is involved in the erosion of trophoblast cells and the activation and migration of immune cells, which plays an important role in inducing pregnancy immune tolerance. The expressed and secreted regulatory proteins play an important role in the immune tolerance in pregnancy (Li et al, 2013;Garcia et al, 2015;Rashidi et al, 2015). The abnormal expression of MCP2 will directly lead to embryonic diapauses or might affect the embryo implantation and growth, indirectly causing abortion.…”

Section: Discussionmentioning

confidence: 99%

Relationship between TLR4 and MCP2 expression levels and habitual abortion

Song

Tian

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Habitual abortion is associated with the altered expression of multiple genes. This study was carried out to investigate the relationship between expression of Toll-like receptor 4 (TLR4) and monocyte chemotactic protein 2 (MCP2 or CCL8) and habitual abortion. This was done by detecting and comparing their relative expression in peripheral blood and placental villi of patients and healthy fertile women. Based on our previous research, 85 subjects with habitual abortion (study group) and 40 healthy fertile women (control group), who were admitted to our hospital between June 2013 and December 2014, were enrolled in this study. After these subjects signed written informed consent, peripheral blood samples and villous tissues were collected, from which the total RNA was extracted. The expression of TLR4 and MCP2 was detected with quantitative reverse transcription-polymerase chain reaction, using GAPDH as a reference control. The expression of TLR4 and MCP2 in the peripheral blood and villous tissues of the study group was significantly higher than that of the control group (P < 0.05). A positive correlation was also 2 X.P. Li et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 15 (2): gmr.15027378 observed between the changes in expression levels of TLR4 and MCP2. In conclusion, TLR4 and MCP2 expression correlated with the occurrence of habitual abortion. Detecting expression changes in TLR4 and MCP2 in the peripheral blood is a feasible method for predicting the occurrence of abortion in women of child-bearing age.

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“…There is evidence that the speed at which the zygotes undergo their mitotic cycles during cleavage also strongly influences future development (GARCIA et al, 2015). However, there is Effect of follicular diameter, time of first cleavage and H3K4 methylation on embryo production rates of Bos indicus cattle discordance among researchers regarding the ideal length of time for the embryo to complete the first cell cycle (LEQUARRE et al, 2003;SUGIMURA et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effect of follicular diameter, time of first cleavage and H3K4 methylation on embryo production rates of Bos indicus cattle

Lunardelli

Marinho

Rosa

et al. 2016

SCA

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This study aimed investigate the relationship between epigenetics, follicular diameter and cleavage speed, by evaluating the developmental potential and occurence of H3K4 monomethylation of early-, intermediate-and late-cleaving Bos indicus embryos from in vitro fertilized oocytes originating from follicles up to 2 mm in diameter or between 4 and 8 mm in diameter. Oocytes (n = 699) from small follicles (≤ 2 mm) and 639 oocytes from large follicles (4-8 mm) were punched from 1,982 Bos indicus' slaughterhouse ovaries. After maturation and in vitro fertilization (IVF), the cultured embryos were separated into early (≤ 28 h post-IVF), intermediate (> 28 h and ≤ 34 h post-IVF) and late (> 34 h and ≤ 54 h post-IVF) cleavage groups. Blastocysts were subjected to an immunofluorescence assessment for H3K4me investigation. The blastocyst rate for large follicles (36.3%) was higher than that for small follicles (22.9%, P<0.05). In addition, blastocyst rates for early and intermediate cleavage groups (45.3% and 33.8%, respectively) were higher than that for late cleavage group (13.5%, P < 0.05). The blastocysts from all groups displayed H3K4me staining by immunofluorescence, particularly intense in what seemed to be trophectoderm cells and weak or absent in cells seemingly from the inner cell mass. For the first time for indicus embryos, data from this study demonstrate that higher blastocyst embryo rates are obtained from embryos that cleave within 34 h after fertilization and from those produced from follicles of 4-8 mm in diameter, indicating a greater ability of these embryos to develop to the stage of embryonic preimplantation. This is the first article demonstrating the occurrence of H3K4me in cattle embryos; its presence in all the evaluated blastocysts suggests that this histone modification plays a key role in maintaining embryo viability at preimplantation stage. Key words: Bovine. Early cleavage. Follicular size. Late cleavage. Histone modifications. ResumoEste estudo teve como objetivo investigar a relação entre a epigenética, o diâmetro folicular e a velocidade de clivagem, avaliando o potencial de desenvolvimento e a ocorrência de monometilação da H3K4 em embriões Bos indicus de clivagem precoce, intermediária e tardia produzidos a partir de oócitos fertilizados in vitro oriundos de folículos de até 2 mm de diâmetro ou entre 4 e 8 mm de diâmetro.

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Developmental Block and Programmed Cell Death in Bos indicus Embryos: Effects of Protein Supplementation Source and Developmental Kinetics

Cited by 22 publications

References 38 publications

Pre- and Peri-/Post-Compaction Follistatin Treatment Increases In Vitro Production of Cattle Embryos

Pre- and Peri-/Post-Compaction Follistatin Treatment Increases In Vitro Production of Cattle Embryos

Relationship between TLR4 and MCP2 expression levels and habitual abortion

Effect of follicular diameter, time of first cleavage and H3K4 methylation on embryo production rates of Bos indicus cattle

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