Developmental Changes in Action Potential Prolongation by K+-Channel Blockers in Chick Myocardium

Nouchi, Hideaki; Kimata, Mikio; Tsuneoka, Yousuke; Hamaguchi, Satoshi; Namekata, Iyuki; Takahara, Akira; Shigenobu, Koki; Tanaka, Hikaru

doi:10.1254/jphs.10205sc

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…Such shortening of the QT interval may be the result of increased expression during embryonic development of the genes encoding the cardiac potassium channels involved in repolarization. This is supported by a study of isolated chick myocardium where the magnitude of action potential prolongation by the IKr blocker E4031 was found to be larger in 7-10 and 11-13 day old embryos compared to 14-20 day old embryos [2]. Moreover, the effect on action potential prolongation with the IKs blocker chromanol 293B was generally small, suggesting that the IKr current is primarily responsible for ventricular repolarization in the chick embryo and that IKr changes gradually during embryonic development.…”

Section: Discussionmentioning

confidence: 61%

Electrocardiographic Measurements of the QT Interval During Embryonic Development in Fertilized Chicken Eggs

Bhuiyan

Sevcencu

Struijk

et al. 2016

2016 Computing in Cardiology Conference (CinC)

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The fertilized chicken egg (embryonic chicken) IntroductionThe ionic basis of the chick embryonic action potential is similar to the most vertebrate hearts and studies of chick embryonic ion channels show that the permeability to K+ increases during the embryonic period [1]. Potassium channels are responsible for the repolarization process and any change in K+ permeability may have an effect on the electrocardiographic QT interval. Changes in ion channel permeability may also greatly affect the responsiveness of the myocardium to pharmacological agents.In this study we have recorded in vivo electrocardiograms from fertilized chicken eggs to investigate the association between embryonic development and the duration of the QT interval. Methods Study populationECGs were recorded from 4 fertilized chicken eggs for 6 days. Between recordings, the fertilized eggs were kept in an incubator with a temperature of 37.4°C. Electrode implantationGold plated chronic electrodes were used. The position of the embryo was confirmed prior to electrode placement by setting the egg against strong coherent light. The electrodes were cleaned in 80% alcohol solution to prevent bacterial formation. Three tiny holes were made with sterile needles and the electrodes were inserted at the equatorial region of the eggs 120 0 apart from each other. The two electrodes placed with the embryo in between them were connected to differential inputs and the third electrode was connected to the ground of the iWorx system (iWorx TM ). After the implantation, the holes were immediately closed with acrylic cement and the egg was put back in the incubator. ECG recordingsContinuous 5-minute ECGs were recorded from each egg on days: 13,14,15,16 and 18. A high pass filter (cutoff frequency = 1 Hz) was used to remove baseline drift and a 15-tap moving average filter was used to smooth the ECG signal. Three measurements were made in noise free segments: Heart rate, QT interval, and the Fridericia corrected QT interval (QTcF), and an average value was calculated. A 10 second segment of a representative ECG recording is shown in figure 1.

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Section: Discussionmentioning

confidence: 61%

Electrocardiographic Measurements of the QT Interval During Embryonic Development in Fertilized Chicken Eggs

Bhuiyan

Sevcencu

Struijk

et al. 2016

2016 Computing in Cardiology Conference (CinC)

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“…Both before and after these time points, Ca 2+ transient decay time was significantly longer. The prolonged APD at 90% repolarization was observed in 14-20-day-old chicken embryos, compared to 1-7-day-old hatched chicks, 36 suggesting a shortening of APD with maturation. Overall, our results suggest that maturation of iCMs take place through 55 days in culture and the Ca 2+ handling and contraction kinetics resembling aged human hearts is observed by day 100 in culture.…”

Section: Mechanical and Functional Maturation Of Icms With Culture Agementioning

confidence: 82%

An aged human heart tissue model showing age-related molecular and functional deterioration resembling the native heart

Acun

Nguyen

Zorlutuna

2018

Preprint

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Deaths attributed to ischemic heart disease increased by 41.7% from 1990 to 2013. This is primarily due to an increase in the aged population, however, research on cardiovascular disease (CVD) has been overlooking aging, a well-documented contributor to CVD. The field heavily depends on the use of young animals due to lower costs and ready availability, despite the prominent differences between young and aged heart structure and function. Here we present the first human induced pluripotent stem cell (hiPSC)-derived cardiomyocyte (iCM)-based, in vitro aged myocardial tissue model as an alternative research platform. Within 4 months, iCMs go through accelerated senescence and show cellular characteristics of aging. Furthermore, the model tissues fabricated using these aged iCMs, with stiffness resembling that of aged human heart, show functional and pharmacological deterioration specific to aged myocardium. Our novel tissue model with age-appropriate physiology and pathology presents a promising new platform for investigating CVD or other age-related diseases.

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Repolarizing potassium currents in working myocardium of Japanese quail: a novel translational model for cardiac electrophysiology

Филатова

Abramochkin

Pavlova

et al. 2021

Comparative Biochemistry and Physiology Part A: Molecular &

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Developmental Changes in Action Potential Prolongation by K+-Channel Blockers in Chick Myocardium

Cited by 3 publications

References 15 publications

Electrocardiographic Measurements of the QT Interval During Embryonic Development in Fertilized Chicken Eggs

Electrocardiographic Measurements of the QT Interval During Embryonic Development in Fertilized Chicken Eggs

An aged human heart tissue model showing age-related molecular and functional deterioration resembling the native heart

Repolarizing potassium currents in working myocardium of Japanese quail: a novel translational model for cardiac electrophysiology

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