Developmental changes in chemotactic response and choice of two attractants, sodium acetate and diacetyl, in the nematode Caenorhabditis elegans

Matsuura, Tetsuya; Endo, Seiko; Iwamoto, Rie; Takahashi, H.; Ichinose, Mitsuyuki

doi:10.1016/j.cbpa.2007.02.023

Cited by 16 publications

(37 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…24,25) Last larval (L4) stage nematodes have a crescent structure around the vulva; this structure disappears in the YA stage and thus was absent in all experimental animals. To obtain synchronous hermaphrodites, 50 gravid nematodes were transferred to fresh NGM plates with or without 0.3 mM nicotine and incubated for 3 h at 25°C to lay eggs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Matsuura

Urushihata

2015

Bioscience, Biotechnology, and Biochemistry

Self Cite

View full text Add to dashboard Cite

The chemotaxis of wild-type NaCl-conditioned nematodes exposed to 100 mM NaCl, maintained on a growth medium containing 0.3 mM nicotine from first larva to young adult (YA) hermaphrodite, was significantly weaker than the chemotaxis of those maintained on a medium without nicotine. The result indicates that chronic nicotine exposure augments gustatory plasticity. The gustatory plasticity was also augmented when tph-1 mutants, with a defect in serotonin biosynthesis, were maintained on a medium containing nicotine until the YA stage. Chronic nicotine exposure did not augment gustatory plasticity in bas-1 mutants, which had defects in both serotonin and dopamine biosynthesis, and in cat-2 mutants, which had a defect in dopamine biosynthesis. However, augmentation of gustatory plasticity was observed when bas-1 and cat-2 mutants were maintained on a growth medium containing nicotine along with dopamine, suggesting that dopamine signaling is involved in the augmentation of gustatory plasticity due to chronic nicotine exposure.

show abstract

Section: Introductionmentioning

confidence: 99%

“…1) at 18 and 3 h before the start of the assay. 23,24,26,27) The assay plate was stored in an incubator at 20°C. A 1 μL of 0.5 M sodium azide was spotted on the same location as that of NaCl shortly before the chemotaxis assay to anesthetize the nematodes.…”

Section: Introductionmentioning

confidence: 99%

Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Matsuura

Urushihata

2015

Bioscience, Biotechnology, and Biochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…2B). It has been reported that the chemotaxis indices for sodium acetate in N2 animals varied by developmental stage (Matsuura et al, 2007a). Therefore, the higher chemotaxis indices of 6 hr and 12 hr may have been caused by changes in sensitivity to sodium acetate over the course of development.…”

Section: Fig 3 (A)mentioning

confidence: 96%

“…To obtain them, fifty gravid animals were moved to a fresh NGM plate, allowed to lay eggs for 3 hr at 25°C, and removed from the plate. The animals developed into YA hermaphrodites approximately 75 hr after the removal of gravid animals at 20°C (Matsuura et al, 2004;2007a;Shingai et al, 2005). Although several hundred YA animals were grown on one NGM plate using this method, there was no effect of population density on chemotaxis index (unpublished observation), suggesting that differences in the population density during developmental period do not affect chemotactic response.…”

Section: Animalsmentioning

confidence: 98%

Enhancement of Chemotactic Response to Sodium Acetate in the NematodeCaenorhabditis elegans

et al. 2010

Self Cite

View full text Add to dashboard Cite

In this study, we investigated the chemotactic response of a wild-type (N2) nematode (Caenorhabditis elegans) to a water-soluble attractant, sodium acetate, after pre-exposure to the chemical. The chemotactic response to 1.0 M sodium acetate of the non-exposed control nematodes was lower than that of the nematodes that were pre-exposed to 1.0 M sodium acetate for 90 min (p < 0.05). The increase in the response to sodium acetate was observed up to 6 hr, but not at 12 hr after exposure. To clarify the mechanism of this enhancement of the chemotactic response, several mutants were used. The chemotactic response of pre-exposed tph-1 and bas-1 mutants, whose main defect was serotonin secretion, was enhanced in comparison with that of the control mutants (p < 0.01). However, cat-1 and cat-2 mutants, which are respectively defective in serotonin and dopamine secretion and dopamine secretion only, showed no enhancement of the chemotactic response to sodium acetate, even when pre-exposed to this chemical. When the cat-1 and cat-2 mutants were pre-exposed to sodium acetate and bred in the presence of 40 mM dopamine, these mutants showed enhanced chemotactic response to sodium acetate (p < 0.05). These results suggest that the enhancement of chemotactic response to sodium acetate after pre-exposure to this chemical is modulated by dopaminergic neurotransmission.

show abstract

“…The nematode shows chemotactic responses to the odorant diacetyl (Matsuura et al, 2005(Matsuura et al, , 2007(Matsuura et al, , 2009Nishino et al, 2013), which is produced by food bacteria. The chemotactic response to diacetyl of young adult (YA) nematodes pre-exposed to this odorant in the absence of food was significantly smaller than that of unexposed control nematodes.…”

Section: Introductionmentioning

confidence: 99%

Extension of the established period of diacetyl adaptation by oxygen intermediates in the nematode Caenorhabditis elegans

Nishino

Satô

Ito

et al. 2015

Comparative Biochemistry and Physiology Part A: Molecular &

Self Cite

View full text Add to dashboard Cite

Developmental changes in chemotactic response and choice of two attractants, sodium acetate and diacetyl, in the nematode Caenorhabditis elegans

Cited by 16 publications

References 25 publications

Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Enhancement of Chemotactic Response to Sodium Acetate in the NematodeCaenorhabditis elegans

Extension of the established period of diacetyl adaptation by oxygen intermediates in the nematode Caenorhabditis elegans

Contact Info

Product

Resources

About