Enhancement of Chemotactic Response to Sodium Acetate in the Nematode<i>Caenorhabditis elegans</i>

Matsuura, Tetsuya; Oda, Takayuki; Hayashi, Genta; Sugisaki, Daisuke; Ichinose, Mitsuyuki

doi:10.2108/zsj.27.629

Cited by 9 publications

(13 citation statements)

References 38 publications

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“…13,17,23,25,32) bas-1(tm351) and bas-1(ad446) mutants are defective in an aromatic amino acid decarboxylase required for serotonin and dopamine production. 33) The chemotaxis index for NaCl of nicotine−/NaCl+ bas-1 mutants was significantly lower than that of nicotine−/NaCl− mutants (p < 0.01).…”

Section: Necessity Of Dopamine Signaling In Augmentation Of Gustatorymentioning

confidence: 99%

“…24,25) Last larval (L4) stage nematodes have a crescent structure around the vulva; this structure disappears in the YA stage and thus was absent in all experimental animals. To obtain synchronous hermaphrodites, 50 gravid nematodes were transferred to fresh NGM plates with or without 0.3 mM nicotine and incubated for 3 h at 25°C to lay eggs.…”

Section: Introductionmentioning

confidence: 99%

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Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Matsuura

Urushihata

2015

Bioscience, Biotechnology, and Biochemistry

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The chemotaxis of wild-type NaCl-conditioned nematodes exposed to 100 mM NaCl, maintained on a growth medium containing 0.3 mM nicotine from first larva to young adult (YA) hermaphrodite, was significantly weaker than the chemotaxis of those maintained on a medium without nicotine. The result indicates that chronic nicotine exposure augments gustatory plasticity. The gustatory plasticity was also augmented when tph-1 mutants, with a defect in serotonin biosynthesis, were maintained on a medium containing nicotine until the YA stage. Chronic nicotine exposure did not augment gustatory plasticity in bas-1 mutants, which had defects in both serotonin and dopamine biosynthesis, and in cat-2 mutants, which had a defect in dopamine biosynthesis. However, augmentation of gustatory plasticity was observed when bas-1 and cat-2 mutants were maintained on a growth medium containing nicotine along with dopamine, suggesting that dopamine signaling is involved in the augmentation of gustatory plasticity due to chronic nicotine exposure.

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Section: Necessity Of Dopamine Signaling In Augmentation Of Gustatorymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Matsuura

Urushihata

2015

Bioscience, Biotechnology, and Biochemistry

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“…In C. elegans , continuous presentation of chemical and mechanical stimuli can cause a decrease in the level of response to the same stimuli [7], [25]–[27]. Recently, Matsuura et al [28] showed that the chemotactic behavior of C. elegans to Sodium Acetate was significantly enhanced when the animals were pre-exposed to the chemical for 90 minutes. This increase was observed for about 6 hours after pre-exposure but not after 12 hours.…”

Section: Introductionmentioning

confidence: 99%

Effect of Temperature Pre-Exposure on the Locomotion and Chemotaxis of C. elegans

2014

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The effect of temperature pre-exposure on locomotion and chemotaxis of the soil-dwelling nematode Caenorhabditis elegans has been extensively studied. The behavior of C. elegans was quantified using a simple harmonic curvature-based model. Animals showed increased levels of activity, compared to control worms, immediately after pre-exposure to 30°C. This high level of activity in C. elegans translated into frequent turns by making ‘complex’ shapes, higher velocity of locomotion, and higher chemotaxis index () in presence of a gradient of chemoattractant. The effect of pre-exposure was observed to be persistent for about 20 minutes after which the behavior (including velocity and ) appeared to be comparable to that of control animals (maintained at 20°C). Surprisingly, after 30 minutes of recovery, the behavior of C. elegans continued to deteriorate further below that of control worms with a drastic reduction in the curvature of the worms' body. A majority of these worms also showed negative chemotaxis index indicating a loss in their chemotaxis ability.

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“…Hence, lead acetate, a soluble lead compound, was used for CE to uptake lead. CE displays short term (<12 h) chemotactic responses to acetate (Matsuura et al 2010). Acetate is used by the nematodes for fatty acids biosynthesis (Rothstein 1972).…”

Section: Methodsmentioning

confidence: 99%

Metabolic profiling in Caenorhabditis elegans provides an unbiased approach to investigations of dosage dependent lead toxicity

et al. 2012

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The nematode, Caenorhabditis elegans (CE), serves as a model system in which to explore the impact of particularly low-levels of lead [250, 500, 1000 and 2000 parts per million (ppm) (1.4 × 10−6 M to 1.1 × 10−5 M/nematode)] on specific metabolic pathways and processes. Chromatographic profiles of redox active metabolites are captured through application of high performance liquid chromatography coupled to electrochemical detection (Coularray/HPLC). Principal Component Analysis (PCA: unbiased cluster analysis) and the application of a slicing program, located significant areas of difference occurring within the 2.8–4.58 min section of the chromatograms. It is within this region of the data profiles that known components of the purine pathway reside. Two analytes of unknown structure were detected at 3.5 and 4 min respectively. Alterations in levels of the purine, tryptophan and tyrosine pathway intermediates measured in response to differing concentrations of lead acetate indicate that the effect of lead on these pathways is not linear, yet the ratio of the pathway precursors, tryptophan and tyrosine remains relatively constant. The application of the above combined analytical approaches enhances the value of data generated. Exposure of CE to very low levels of lead produced significant alterations in profiles of electrochemically active compounds.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-012-0438-0) contains supplementary material, which is available to authorized users.

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Enhancement of Chemotactic Response to Sodium Acetate in the NematodeCaenorhabditis elegans

Cited by 9 publications

References 38 publications

Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Chronic nicotine exposure augments gustatory plasticity inCaenorhabditis elegans:involvement of dopamine signaling

Effect of Temperature Pre-Exposure on the Locomotion and Chemotaxis of C. elegans

Metabolic profiling in Caenorhabditis elegans provides an unbiased approach to investigations of dosage dependent lead toxicity

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