Developmental changes in circulating IL-8/CXCL8 isoforms in neonates

Maheshwari, Akhil; Voitenok, Nikolai N.; Akalovich, Svetlana; Shaik, Sadiq S.; Randolph, David A.; Sims, Brian; Patel, Rakesh P.; Killingsworth, Cheryl R.; Fallon, Michael B.; Ohls, Robin K.

doi:10.1016/j.cyto.2008.12.022

Cited by 25 publications

(23 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most recently, studies have demonstrated other unique functional features of the newborn immune system, potentially allowing age-specific interactions between innate and adaptive immune cells during the course of an infection. For example, neonatal immune cells can produce developmentally unique cytokine isoforms, and two examples have been reported in humans with IL-4 and CXCL8 (also known as interleukin-8 (IL-8) [32,33]. In addition, a large proportion of human naïve neonatal T cells are able to produce the CXCL8 chemokine upon stimulation [34].…”

Section: Box 2 Relative Importance Of the Innate And Adaptive Immune mentioning

confidence: 99%

An Immunological Perspective on Neonatal Sepsis

Kan

Razzaghian

Lavoie

2016

Trends in Molecular Medicine

View full text Add to dashboard Cite

Despite concerted international efforts, mortality from neonatal infections remains unacceptably high in some areas of the world, particularly for premature infants. Recent developments in flow cytometry and next-generation sequencing technologies have led to major discoveries over the past few years, providing a more integrated understanding of the developing human immune system in the context of its microbial environment. We review these recent findings, focusing on how in human newborns incomplete maturation of the immune system before a full term of gestation impacts on their vulnerability to infection. We also discuss some of the clinical implications of this research in guiding the design of more-accurate age-adapted diagnostic and preventive strategies for neonatal sepsis.

show abstract

Section: Box 2 Relative Importance Of the Innate And Adaptive Immune mentioning

confidence: 99%

An Immunological Perspective on Neonatal Sepsis

Kan

Razzaghian

Lavoie

2016

Trends in Molecular Medicine

View full text Add to dashboard Cite

show abstract

“…However, several lines of evidence indicate that IL-8 and TGF-␤ may not be important as macrophage chemoattractants in the fetal intestine. In the fetus, IL-8 is comprised mainly of a longer, less-potent 77-amino acid isoform (unlike the shorter 72-amino acid isomer in the adult) (35). Similarly, TGF-␤ bioactivity is low in the early-and midgestation fetal intestine (A. Maheshwari, N. Ambalavanan, T. Nicola, D. R. Kelly, J. M. Murphy-Ullrich, M. Athar, M. Shimamura, V. Bhandari, C. Aprahamian, R. A. Dimmitt, R. Serra, and R. K. Ohls, unpublished observations).…”

mentioning

confidence: 99%

Epithelial cells in fetal intestine produce chemerin to recruit macrophages

Maheshwari

Kurundkar

Shaik

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

Self Cite

View full text Add to dashboard Cite

Macrophages are first seen in the fetal intestine at 11-12 wk and rapidly increase in number during the 12- to 22-wk period of gestation. The development of macrophage populations in the fetal intestine precedes the appearance of lymphocytes and neutrophils and does not require the presence of dietary or microbial antigens. In this study, we investigated the role of chemerin, a recently discovered, relatively selective chemoattractant for macrophages, in the recruitment of macrophage precursors to the fetal intestine. Chemerin mRNA/protein expression was measured in jejunoileal tissue from 10- to 24-wk human fetuses, neonates operated for intestinal obstruction, and adults undergoing bariatric surgery. The expression of chemerin in intestinal epithelial cells (IECs) was confirmed by using cultured primary IECs and IEC-like cell lines in vitro. The regulatory mechanisms involved in chemerin expression were investigated by in silico and immunolocalization techniques. IECs in the fetal, but not mature, intestine express chemerin. Chemerin expression peaked in the fetal intestine at 20-24 wk and then decreased to original low levels by full term. During the 10- to 24-wk period, chemerin accounted for most of the macrophage chemotactic activity of cultured fetal IECs. The maturational changes in chemerin expression correlated with the expression of retinoic acid receptor-beta in the intestine. Chemerin is an important mediator of epithelial-macrophage cross talk in the fetal/premature, but not in the mature, intestine. Understanding the regulation of the gut macrophage pool is an important step in development of novel strategies to boost mucosal immunity in premature infants and other patient populations at risk of microbial translocation.

show abstract

“…Furthermore, in NEC-affected tissue, IL-8, a member of the CXC chemokine family with chemotactic activity, is found in excessive amounts and has been speculated to be associated with severe inflammatory conditions (5,21). However, in the fetal intestine, IL-8 exerts other effects, stimulating maturation, but without associated inflammation (23). IL-8 also stimulates intestinal cell proliferation, differentiation, migration, protection from injury, and prevention from cell death (20,22).…”

mentioning

confidence: 99%