Developmental changes in intercellular junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods

Wang, Jing; Zeng, Liming; Tan, Bin; Li, Guangran; Huang, Bo; Xiong, Xia; Li, Fengna; Kong, Xiangfeng; Liu, Gang; Yin, Yulong

doi:10.1186/s40104-016-0063-2

Cited by 72 publications

(78 citation statements)

References 47 publications

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“…The results from the present study confirmed that piglet growth performance, including ADFI, was enhanced with the increase in post‐weaning time (Wang et al, ; Xiccato, Trocino, Sartori, & Queaque, ). The SCFAs, mostly acetate, propionate and butyrate, are produced via the fermentation of dietary carbohydrates and proteins in the colon (Parrett et al, ).…”

Section: Discussionsupporting

confidence: 87%

The production of short chain fatty acid and colonic development in weaning piglets

Shi

Zhang

et al. 2019

Animal Physiology Nutrition

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Weaning process widely affects the small intestinal structure and function in piglets, while the responses of large intestine to weaning stress are still obscure. The purpose of this study was to determine the developmental changes (i.e., short chain fatty acids (SCFAs) concentrations, growth parameters, crypt‐related indices and antioxidant capacity) in colon of piglet during weaning. Forty piglets were weaned at day 21 and euthanized to collect colonic tissues and digesta samples on day 0, 1, 3, 7 and 14 post‐weaning (n = 8). Piglet growth performance was improved (p < .001) on day 7 and 14 post‐weaning. The concentrations of acetate, propionate, butyrate, valerate, isobutyrate, isovalerate and total SCFAs were higher (p < .001) during the late post‐weaning period. The mRNA abundances of SCFAs transporters were greater (p < .001) on day 7 and 14. The absolute and relative weights, absolute length and perimeter of colon were greater (p < .001) on day 7 and 14. Similarly, post‐weaning increases (p < .001) in colonic crypt depth and Ki67 positive cells numbers per crypt were observed during the same period. Colonic crypt fission indices decreased (p < .01), while total crypt numbers increased (p < .001) on day 14 after weaning. Moreover, total SCFAs concentration was significantly associated with colonic growth parameters and Ki67 cells/crypt (p < .001). In addition, catalase content was decreased on day 3, 7, and 14, whereas, the concentrations of total superoxide dismutase (T‐SOD) and manganese‐containing superoxide dismutase (MnSOD) were higher (p < .05) on day 1 and 3 post‐weaning. These results showed that weaning process has a significant effect on colonic growth and development, which might be associated with the change of SCFAs concentrations in colon.

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Section: Discussionsupporting

confidence: 87%

The production of short chain fatty acid and colonic development in weaning piglets

Shi

Zhang

et al. 2019

Animal Physiology Nutrition

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“…This drop is reflected in a concomitantly increased permeability for FD-4 and HRP. Previous studies showed lower abundances of occludin mRNA and protein, claudin-3 protein, and increased lactulose permeability after weaning [50, 76]. Thus artificial rearing induced a similar response as seen after conventional weaning.…”

Section: Discussionsupporting

confidence: 57%

Artificial rearing influences the morphology, permeability and redox state of the gastrointestinal tract of low and normal birth weight piglets

Vergauwen

Degroote

Prims

et al. 2017

J Animal Sci Biotechnol

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BackgroundIn this study the physiological implications of artificial rearing were investigated. Low (LBW) and normal birth weight (NBW) piglets were compared as they might react differently to stressors caused by artificial rearing. In total, 42 pairs of LBW and NBW piglets from 16 litters suckled the sow until d19 of age or were artificially reared starting at d3 until d19 of age. Blood and tissue samples that were collected after euthanasia at 0, 3, 5, 8 and 19 d of age. Histology, ELISA, and Ussing chamber analysis were used to study proximal and distal small intestine histo-morphology, proliferation, apoptosis, tight junction protein expression, and permeability. Furthermore, small intestine, liver and systemic redox parameters (GSH, GSSG, GSH-Px and MDA) were investigated using HPLC.ResultsLBW and NBW artificially reared piglets weighed respectively 40 and 33% more than LBW and NBW sow-reared piglets at d19 (P < 0.01). Transferring piglets to a nursery at d3 resulted in villus atrophy, increased intestinal FD-4 and HRP permeability and elevated GSSG/GSH ratio in the distal small intestine at d5 (P < 0.05). GSH concentrations in the proximal small intestine remained stable, while they decreased in the liver (P < 0.05). From d5 until d19, villus width and crypt depth increased, whereas PCNA, caspase-3, occludin and claudin-3 protein expressions were reduced. GSH, GSSG and permeability recovered in artificially reared piglets (P < 0.05).ConclusionThe results suggest that artificial rearing altered the morphology, permeability and redox state without compromising piglet performance. The observed effects were not depending on birth weight.

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“…Third, microscopes were used to observe the samples, and computer-aided systems were used to photograph the samples. Finally, an image analysis system was used to measure villi length and crypt length [23,24].…”

Section: Animal Management and Samplementioning

confidence: 99%

The Evaluation of the Antioxidant and Intestinal Protective Effects of Baicalin-Copper in Deoxynivalenol-Challenged Piglets

Zha

Yuan

Cui

et al. 2020

Oxidative Medicine and Cellular Longevity

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The present study was performed to evaluate the antioxidant and intestinal protective effects of baicalin-copper on deoxynivalenol-challenged piglets. Forty weaned piglets were randomly divided into four groups and assigned to different diets: (1) basal diet (Con), (2) 4 mg/kg deoxynivalenol of basal diet (DON), (3) 5 g/kg baicalin-copper of basal diet (BCU); and (4) 4 mg/kg deoxynivalenol+5 g/kg baicalin‐copper of basal diet (DBCU). The results showed that the ADFI and ADG of piglets in the DON group were markedly lower than those in the Con group, but the ADFI and ADG of the DBCU group were not significantly different from those of the Con group. In piglets fed a DON-contaminated diet, dietary supplementation with BCU significantly decreased the mRNA levels of P70S6K, 4E-BP1, and HSP70 in the liver, the protein expression of HO-1 in the jejunum, and the expression of p-Nrf2 and p-NF-κB in the ileum but increased Mn-SOD activity in serum. Dietary supplementation with BCU increased jejunal maltase, ZIP4 and MT mRNA levels, and serum concentrations of Arg, Val, Ile, Leu, Lys, and Tyr in DON-contaminated piglets. In summary, BCU can alleviate the growth impairment induced by DON and enhance antioxidant capacity and nutrition absorption in piglets fed DON-contaminated diets.

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Developmental changes in intercellular junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods

Cited by 72 publications

References 47 publications

The production of short chain fatty acid and colonic development in weaning piglets

The production of short chain fatty acid and colonic development in weaning piglets

Artificial rearing influences the morphology, permeability and redox state of the gastrointestinal tract of low and normal birth weight piglets

The Evaluation of the Antioxidant and Intestinal Protective Effects of Baicalin-Copper in Deoxynivalenol-Challenged Piglets

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