Developmental Changes in Mucous Cells of the Early Postnatal Rat Parotid Gland: An Ultrastructural and Histochemical Study.

Ikeda, Rie; Aiyama, Shigeo

doi:10.1679/aohc.60.185

Cited by 22 publications

(11 citation statements)

References 24 publications

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“…The salivary glands are said to be predominant in the developmental stage, and that in gland formation the mucous gland is predominant and has a large number of cells. These findings are similar to the findings of Ikeda & Aiyama (1997).…”

Section: Discussionsupporting

confidence: 91%

Investigation of the Effect of GnRH (Deslorelin) on the Histochemical Structure of Salivary Glands in Rats

2019

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This study was planned to determine the histochemical alterations of the submandibular gland by implantation of long-term GnRH (deslorelin 4.7 mg). Eighteen Wistar albino rats were used in the study. Alcian blue (AB; pH: 2.5), periodic acid-Schiff (PAS) staining was performed to determine the microscopic structure and histochemical structure of the GI submandibular gland. The Avidin-Biotin Complex (ABC) method was used to determine the immunohistochemical reactivity of lectin. After GnRH implantation, the organs were examined and atrophies were observed in organs. In the group in which the implants were removed, it was determined that there was no atrophy; organ structures and microscopic examination were similar to the control group. At the end of the study, submandibular gland was fixed in 10 % buffered formaldehyde. In three groups, PAS and AB histochemical staining revealed similar reactions. Immunohistochemically, lectin activity was found to react positively.

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Section: Discussionsupporting

confidence: 91%

Investigation of the Effect of GnRH (Deslorelin) on the Histochemical Structure of Salivary Glands in Rats

2019

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“…Parotid glands are purely serous in adult rats, whereas mucous-type acinar cells are present in neonate rat parotid glands (days 1–8) [7]. In contrast, the sublingual glands of adult rats are mainly mucous.…”

Section: Histology and Organogenesis Of The Salivary Glandsmentioning

confidence: 99%

Capability of Tissue Stem Cells to Organize into Salivary Rudiments

Okumura

Shinohara

Endo

2012

Stem Cells International

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Branching morphogenesis (BrM), an essential step for salivary gland development, requires epithelial-mesenchymal interactions. BrM is impaired when the surrounding mesenchyme is detached from the salivary epithelium during the pseudoglandular stage. It is believed that the salivary mesenchyme is indispensable for BrM, however, an extracellular matrix gel with exogenous EGF can be used as a substitute for the mesenchyme during BrM in the developing salivary epithelium. Stem/progenitor cells isolated from salivary glands in humans and rodents can be classified as mesenchymal stem cell-like, bone-marrow-derived, duct cell-like, and embryonic epithelium-like cells. Salivary-gland-derived progenitor (SGP) cells isolated from duct-ligated rats, mice, and swine submandibular glands share similar characteristics, including intracellular laminin andα6β1-integrin expression, similar to the embryonic salivary epithelia during the pseudoglandular stage. Progenitor cells also isolated from human salivary glands (human SGP cells) having the same characteristics differentiate into hepatocyte-like cells when transplanted into the liver. Similar to the dissociated embryonic salivary epithelium, human SGP cells aggregate to self-organize into branching organ-like structures on Matrigel plus exogenous EGF. These results suggest the possibility that tissue stem cells organize rudiment-like structures, and the embryonic cells that organize into whole tissues during development are preserved even in adult tissues.

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“…Several workers have described the transient appearance of occasional cells with mucous secretory granules in the developing rat parotid gland at ages 1 through 8 days after birth (Lawson,1970; Taga and Sesso,1979; Ikeda and Aiyama,1997,1999). These secretory granules stain intensely with both periodic acid–Schiff and Alcian blue, indicating that they are rich in both neutral and acidic glycoproteins.…”

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confidence: 99%

Effects of Exogenous Thyroid Hormone on the Postnatal Morphogenesis of the Rat Parotid Gland

Ikeda

Aiyama

Redman³

2007

The Anatomical Record

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Administration of thyroid hormone has been shown to accelerate the early postnatal development of the rat parotid gland, but these studies have dwelt almost entirely on biochemical changes. The objective of this study was to describe the effects of exogenous thyroid hormone on morphologic aspects of the developing parotid gland, in particular the transient appearance of scattered mucous cells in this otherwise serous gland. Pups were given a daily subcutaneous injection of thyroxine (T(4)) of 0.1, 0.5, or 5.0 microg/g body weight, vehicle only (injection control), or no injection (normal control) beginning at 4 days, and killed for the collection of blood and parotid glands at intervals through 15 days. The serum was analyzed for T(4) and the glands were examined by light and electron microscopy. The results indicated that both serum T(4) and the pace of gland development were proportional to the dose of T(4). In particular, T(4) accelerated decreases in acinar size and gland area occupied by stroma and translocation of a subset of cells with small secretory granules, deeply stained with periodic acid-Schiff, from acini to intercalated ducts. However, the chronology of mucous cell disappearance was indifferent to treatment. In addition, signs of toxicity, including slower gain in body weight and greatly increased apoptosis and vacuoles in the glands, occurred with the higher doses of T(4).

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Developmental Changes in Mucous Cells of the Early Postnatal Rat Parotid Gland: An Ultrastructural and Histochemical Study.

Cited by 22 publications

References 24 publications

Investigation of the Effect of GnRH (Deslorelin) on the Histochemical Structure of Salivary Glands in Rats

Investigation of the Effect of GnRH (Deslorelin) on the Histochemical Structure of Salivary Glands in Rats

Capability of Tissue Stem Cells to Organize into Salivary Rudiments

Effects of Exogenous Thyroid Hormone on the Postnatal Morphogenesis of the Rat Parotid Gland

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