Developmental Changes in the Microsomal Monooxygenase System and the In Vivo Metabolism of Aldrin in Larvae of the Mexican Bean Beetle (Coleoptera: Coccinellidae)

Jesudason, Princy; Levi, Patricia E.; Weiden, M. H. J.; Roe, Michael R.

doi:10.1093/jee/81.6.1598

Cited by 7 publications

(2 citation statements)

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“…Activity of glutathione S‐ transferases was assayed by measuring the conjugation of two different substrates, viz., 3,4‐dichloronitrobenzene (DCNB) and 1‐chloro‐2,4‐dinitrobenzene (CDNB), based on Yu (1982, 1984), because the activity towards these may vary among different isozymes of glutathione S‐ transferases (Commandeur et al., 1995; Udomsinprasert & Ketterman, 2002). The contents of cytochrome P420 and P450 were determined following Omura & Sato (1964), as modified by Jesudason et al. (1988).…”

Section: Methodsmentioning

confidence: 99%

Glutathione S‐transferase detoxification as a potential pyrethroid resistance mechanism in the maize weevil, Sitophilus zeamais

Fragoso

Guedes

Rezende

2003

Entomologia Exp Applicata

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Insecticide resistance patterns among 16 Brazilian populations of the maize weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), were recognized by surveying resistance to three organophosphates (chlorpyrifos‐methyl, malathion, and pirimiphos‐methyl) and three pyrethroids (cypermethrin, deltamethrin, and permethrin). Two population clusters were obtained: one with three populations (Bragança Paulista, Cristalina, and Nova Andradina) showing low frequency of cypermethrin resistance (13–36%) and negligible frequency of deltamethrin resistance (2–9%); and another with six populations (Campos dos Goytacazes, Ivinhema, Patos de Minas, Penápolis, Uberlândia, and Venda Nova) showing low to negligible levels of pyrethroid resistance (0–23%). The remaining seven populations, including a susceptible, and a DDT‐ and pyrethroid‐resistant reference populations (Sete Lagoas and Jacarezinho, respectively), were significantly different from each other and from the two recognized clusters. In contrast with pyrethroid resistance, organophosphate resistance was negligible except for chlorpyrifos‐methyl in two populations (Fátima do Sul and Penápolis). There was no correlation between geographic distance and the Mahalanobis distance estimated from the resistance pattern ordination of the populations by canonical variate analysis, suggesting local selection and/or broad dispersal of resistant populations by grain trade. The results of biochemical in vitro studies measuring the activity of detoxification enzymes (esterases and glutathion S‐transferases) in conjunction with canonical correlation analysis suggest a major involvement of enhanced conjugation by glutathione S‐transferases (> 2‐fold increase) in pyrethroid resistance and, in the case of cypermethrin resistance, enhanced phosphotriesterase activity.

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Section: Methodsmentioning

confidence: 99%

Glutathione S‐transferase detoxification as a potential pyrethroid resistance mechanism in the maize weevil, Sitophilus zeamais

Fragoso

Guedes

Rezende

2003

Entomologia Exp Applicata

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“…The contents of cytochrome b., P420, and P450 were determined following Omura & Sato (1964) as modified by Jesudason et al (1988). O-demethylase activity was assayed by methods of Hansen & Hodgson (1971) using 0.5 ml of insect homogenate and the substrate p-nitroanisole.…”

Section: Enzyme Assaysmentioning

confidence: 99%

Biochemical mechanisms of organophosphate resistance inRhyzopertha dominica(Coleoptera: Bostrichidae) populations from the United States and Brazil

Guedes

Kambhampati

Dover

et al. 1997

Bull. Entomol. Res.

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Four possible biochemical mechanisms of organophosphate resistance were examined in populations of Rhyzopertha dominica (Fabricius) collected from eight locations in the states of Minas Gerais and Sao Paulo in Brazil and from seven locations in northeast Kansas, United States. Results from insecticide bioassays with synergists (triphenyl phosphate, diethylmaleate, and piperonyl butoxide) suggested that metabolic detoxification mechanisms might be involved in organophosphate resistance in some of the populations of R. dominica. However, overall low synergism, non-synergism or even antagonism presented by these synergists in all 15 resistant populations suggested that carboxylesterases, cytochrome P450 monooxygenases and glutathione S-transferases were not likely to play a major role conferring organophosphate resistance in R. dominica. This hypothesis was strongly supported by our biochemical studies showing no significant difference in these enzyme activities, as well as cytochrome b-,, P420, and P450 levels between the susceptible and resistant populations. In contrast, all resistant populations of R. dominica showed higher acetylcholinesterase activity than the susceptible population both in the presence and absence of malaoxon. Furthermore, acetylcholinesterase from the resistant populations was less sensitive to inhibition by malaoxon than that from the susceptible. These results suggested that quantitative and qualitative changes in acetylcholinesterase might contribute to organophosphate resistance in these populations of R. dominica.

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Distribution and metabolism of abamectin in larval stages of Spodoptera littoralis boisduval and Heliothis armigera hübner (Lepidoptera: Noctuidae)

Christie

Wright

1991

Pestic. Sci.

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Comparisons were made between the amounts of abamectin and total radioactivity recovered from Spodoptera littoralis and Heliothis armigera larvae after topical application of [3aabamectin. Penetration (as shown by wash-off experiments) did not differ significantly between the Spodoptera instars. Signijicantly more abamectin was recovered from ventral nerve cord samples of larvae showing symptoms of poisoning than from larvae not showing these symptoms. Fifth-instar S . littoralis larvae had a significantly lower proportion of radioactivity as abamectin in the ventral nerve cord than in sixth-instar S . littoralis or fiyth-and sixth-instar H . armigera. The proportion of radioactivity present as abamectin (but not total radioactivity) was significantly increased when the fi'j-th-instar S. littoralis larvae were pre-treated with piperonyl butoxide (PB) suggesting that the relative insensitivity of fiyth-instar S. littoralis larvae to abamectin is due at least in part to greater metabolism, particularly b-v microsomal oxidases. Fat body samples consistently had a greater proportion of radioactivity as abamectin than the nerve cord and the former may act as storage sites for abamectin .

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Developmental Changes in the Microsomal Monooxygenase System and the In Vivo Metabolism of Aldrin in Larvae of the Mexican Bean Beetle (Coleoptera: Coccinellidae)

Cited by 7 publications

References 0 publications

Glutathione S‐transferase detoxification as a potential pyrethroid resistance mechanism in the maize weevil, Sitophilus zeamais

Glutathione S‐transferase detoxification as a potential pyrethroid resistance mechanism in the maize weevil, Sitophilus zeamais

Biochemical mechanisms of organophosphate resistance inRhyzopertha dominica(Coleoptera: Bostrichidae) populations from the United States and Brazil

Distribution and metabolism of abamectin in larval stages of Spodoptera littoralis boisduval and Heliothis armigera hübner (Lepidoptera: Noctuidae)

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