Developmental changes of aggrecan, versican and neurocan in the retina and optic nerve

Popp, Susanna; Maurel, Patrice; Andersen, Julie S.

doi:10.1016/j.exer.2004.05.016

Cited by 33 publications

(26 citation statements)

References 13 publications

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“…Versican is reported to be mainly localized in white matter regions (Popp et al 2004;Schmalfeldt et al 2000;Oohashi et al 2002). We have also observed versican in the white matter and have detected GAGα immunoreactivity in the corpus callosum, anterior commissure, lateral olfactory tracts, optic tracts, striatal white matter, cerebellar white matter, internal capsule, and fimbria of hippocampus.…”

Section: Discussionmentioning

confidence: 52%

Localization of chondroitin sulfate proteoglycan versican in adult brain with special reference to large projection neurons

et al. 2008

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Versican is a chondroitin sulfate proteoglycan belonging to the lectican family. Versican has two glycosaminoglycan attachment regions, named the GAG alpha and GAG beta domains, which are both regulated by alternative splicing and yield four protein isoforms. We have investigated the expression and localization of versican in the developing and adult brain by using anti-versican GAG alpha and GAG beta antibodies. Western analysis revealed that GAG alpha-reactive isoform was dominant in the adult brain. Immunohistochemical study demonstrated that GAG alpha immunoreactivity was detectable from neonatal periods to adulthood, whereas GAG beta immunoreactivity completely disappeared within 3 weeks of birth. In the adult brain, GAG alpha immunoreactivity was seen in the white matter regions and was also localized in the gray matter including somata and dendrites of cortical and hippocampal pyramidal neurons and cerebellar Purkinje cells. In contrast, GAG alpha immunoreactivity was not localized on parvalbumin-positive interneurons and cerebellar stellate cells. Furthermore, GAG alpha immunoreactivity was not co-localized with perineuronal net markers such as Wisteria floribunda agglutinin lectin and phosphacan. Thus, versican was localized on large projection neurons rather than small interneurons. To confirm the binding mechanism of versican to neurons, hyaluronan and chondroitin sulfates were enzymatically removed from brain sections before the immunolabeling of versican. These treatments had no effect on the labeling pattern of versican, suggesting that other versican-interactive molecules are involved in the binding of versican to neurons.

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Section: Discussionmentioning

confidence: 52%

Localization of chondroitin sulfate proteoglycan versican in adult brain with special reference to large projection neurons

et al. 2008

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“…There are few reports on distribution of CSPGs in the retina and optic nerve [18,31,45]. In this study, we estimated the presence and distribution of C4S, C6S, aggrecan and biglycan in addition to keratan sulfate.…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal Distribution of Chondroitin Sulfate Proteoglycans in the Developing Mouse Retina and Optic Nerve

Ali

Hosaka

Uehara

2011

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of the present study was to determine the distribution of chondroitin sulfate proteoglycans in the mouse retina and optic nerve of the prenatal and postnatal mouse by immunohistochemistry. At embryonic day (E) 18, chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S) and biglycan were detected in the retina and optic nerve. However, aggrecan was seen in the retina but not in the optic nerve. At postnatal day (P) 7, aggrecan and biglycan were clearly observed in the optic nerve, inner nuclear layer and ganglion cell layer and diffuse in the outer retina. C4S diffusely distributed in the retina and optic nerve, but C6S was mainly confined to the photoreceptor layer and optic nerve sheath. At P42, biglycan showed diffuse distribution in the retina and optic nerve with intense staining in nerve-fiber rich layers. Aggrecan showed weak staining at the inner plexiform layer with higher density in the outer and inner nuclear layers, outer plexiform layer and ganglion cell layer. Both C4S and C6S were detected in the optic nerve and retina, but C6S showed strong immunostaining in the photoreceptor layer. The distributions of these proteoglycans with respect of time course during development of the retina and optic nerve suggest that they may have unique or overlapping roles in development and maintenance of the retina and optic nerve.KEY WORDS: chondroitin sulfate proteoglycan, development, optic nerve, retina, spatiotemporal distribution.

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“…Subsequent studies documented its presence in the developing and adult CNS (Milev et al, 1998;Schmalfeldt et al, 1998;Popp et al, 2003Popp et al, , 2004Schweigreiter et al, 2004). In general, versican is believed to be synthesized primarily by glial cells Niederöst et al, 1999;Asher et al, 2002) and to be expressed at higher levels in postnatal than in embryonic brain (Bignami et al, 1993;Milev et al, 1998).…”

Section: Versican As a Laminar Markermentioning

confidence: 99%

“…Prominent components of the ECM of the brain are chondroitin sulfate proteoglycans (CSPGs) of the "lectican" family: aggrecan, brevican, neurocan, and versican (Bignami et al, 1993;Matsui et al, 1998;Milev et al, 1998;Schmalfeldt et al, 1998;Hagihara et al, 1999;Yamaguchi, 2000;Matthews et al, 2002;Popp et al, 2003Popp et al, , 2004. Recent studies have implicated lecticans in two phenomena in the postnatal brain.…”

Section: Introductionmentioning

confidence: 99%

Versican in the Developing Brain: Lamina-Specific Expression in Interneuronal Subsets and Role in Presynaptic Maturation

Yamagata¹,

Sanes²

2005

J. Neurosci.

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Chondroitin sulfate proteoglycans (CSPGs) of the extracellular matrix help stabilize synaptic connections in the postnatal brain and impede regeneration after injury. Here, we show that a CSPG of the lectican family, versican, also promotes presynaptic maturation in the developing brain. In the embryonic chick optic tectum, versican is expressed selectively by subsets of interneurons confined to the retinorecipient laminae, in which retinal axons arborize and form synapses. It is a major receptor for the Vicia villosa B 4 lectin (VVA), shown previously to inhibit invasion of the retinorecipient lamina by retinal axons (Inoue and Sanes, 1997). In vitro, versican promotes enlargement of presynaptic varicosities in retinal axons. Depletion of versican in ovo, by RNA interference, results in retinal arbors with smaller than normal varicosities. We propose that versican provides a lamina-specific cue for presynaptic maturation and discuss the related but distinct effects of versican depletion and VVA blockade.

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Developmental changes of aggrecan, versican and neurocan in the retina and optic nerve

Cited by 33 publications

References 13 publications

Localization of chondroitin sulfate proteoglycan versican in adult brain with special reference to large projection neurons

Localization of chondroitin sulfate proteoglycan versican in adult brain with special reference to large projection neurons

Spatiotemporal Distribution of Chondroitin Sulfate Proteoglycans in the Developing Mouse Retina and Optic Nerve

Versican in the Developing Brain: Lamina-Specific Expression in Interneuronal Subsets and Role in Presynaptic Maturation

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