Developmental expression of keratan sulfate-like immunoreactivity distinguishes thalamic nuclei and cortical domains

Miller, Bradley D.; Sheppard, Allan; Pearlman, Alan L.

doi:10.1002/(sici)1096-9861(19970421)380:4<533::aid-cne9>3.0.co;2-2

Cited by 25 publications

(16 citation statements)

References 78 publications

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“…2B). Robust expression of the 5D4 epitope in the subplate and marginal zone of P1 cortex was observed, as previously described for rat tissue (Miller et al 1997) (Fig. 2C).…”

Section: Deficient Expression Of the 5d4 Ks Epitope In Early Postnatasupporting

confidence: 85%

KSGal6ST Is Essential for the 6-Sulfation of Galactose within Keratan Sulfate in Early Postnatal Brain

Hoshino

Foyez

Ohtake-Niimi

et al. 2013

J Histochem Cytochem.

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SummaryKeratan sulfate (KS) comprises repeating disaccharides of galactose (Gal) and N-acetylglucosamine (GlcNAc). Residues of Gal and GlcNAc in KS are potentially modified with sulfate at their C-6 positions. The 5D4 monoclonal antibody recognizes KS structures containing Gal and GlcNAc, both 6-sulfated, and has been used most extensively to evaluate KS expression in mammalian brains. We previously showed that GlcNAc6ST1 is an enzyme responsible for the synthesis of the 5D4 epitope in developing brain and in the adult brain, where it is induced after injury. It has been unclear which sulfotransferase is responsible for Gal-6-sulfation within the 5D4 KS epitope in developing brains. We produced mice deficient in KSGal6ST, a Gal-6-sulfotransferase. Western blotting and immunoprecipitation revealed that all 5D4-immunoreactivity to proteins, including phosphacan, were abolished in KSGal6ST-deficient postnatal brains. Likewise, the 5D4 epitope, expressed primarily in the cortical marginal zone and subplate and dorsal thalamus, was eliminated in KSGal6ST-deficient mice. Disaccharide analysis showed the loss of Gal-6-sulfate in KS of the KSGal6ST-deficient brains. Transfection studies revealed that GlcNAc6ST1 and KSGal6ST cooperated in the expression of the 5D4 KS epitope in HeLa cells. These results indicate that KSGal6ST is essential for C-6 sulfation of Gal within KS in early postnatal brains. (J Histochem Cytochem 62:145-156, 2014)

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“…2B). Robust expression of the 5D4 epitope in the subplate and marginal zone of P1 cortex was observed, as previously described for rat tissue (Miller et al 1997) (Fig. 2C).…”

Section: Deficient Expression Of the 5d4 Ks Epitope In Early Postnatasupporting

confidence: 85%

KSGal6ST Is Essential for the 6-Sulfation of Galactose within Keratan Sulfate in Early Postnatal Brain

Hoshino

Foyez

Ohtake-Niimi

et al. 2013

J Histochem Cytochem.

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“…Developmental expression of KSs in the brain has been reported in chick (Cole & McCabe, 1991), mice (Hoshino et al, 2014) and rats (Miller et al, 1997). Previous studies suggest that KS is involved in neuronal regeneration and sprouting.…”

Section: Discussionmentioning

confidence: 98%

Time‐dependent localization of high‐ and low‐sulfated keratan sulfates in the song nuclei of developing zebra finches

Fujimoto

Ohgomori

Abe

et al. 2015

Eur J of Neuroscience

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Keratan sulfate proteoglycans (KSPGs) and chondroitin sulfate proteoglycans (CSPGs) consist of a protein core with covalently attached glycosaminoglycan side chain. Although CSPGs are known to regulate the end of the critical period, the role of KSPGs in brain development remains unclear. Young male zebra finches memorise song templates during development. The brain regions that are responsible for song learning, known as song nuclei, are recognized as a suitable model for the study of brain development. To understand the potential role of KSPGs, here we examined the localization of KSs with different degrees of sulfation in the brain of developing male zebra finches. Exclusively in the song nuclei, an increase in expression of 5-D-4-positive (5-D-4(+)) high-sulfated KS started after hatching, and reached a plateau at the end of the sensory period, during which the young bird listens to and memorises the song of an adult tutor. By contrast, weak and ubiquitous expression of BCD-4(+) low-sulfated KS remained unchanged until the end of the sensory period, and first increased in the song nuclei at the end of the sensorimotor period, during which the young bird produces plastic songs. Immunoblot analysis showed that phosphacan was a common core protein of 5-D-4(+) KS and BCD-4(+) KS. Finally, we confirmed that the sulfotransferase responsible for the synthesis of high-sulfated KS was exclusively localised in the song nuclei. Our observations suggest that time-dependent localization of KSPGs with different sulfation patterns in the song nuclei may underlie song learning in developing male zebra finches.

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“…Indeed, bovine cornea alone contains three different KSPG proteins Funderburgh et al 1991). Although the exact identities of the proteoglycans recognised by 5D4 are still unknown (see Miller et al 1997), the specificity of the binding is nevertheless guaranteed by the quality of the antibody which is well characterised (Caterson et al 1983;Mehmet et al 1986) and by the absence of labelling after specific enzymatic digestion.…”

Section: Discussionmentioning

confidence: 99%

Regulated expression of keratan sulphate and peanut agglutinin binding sites during organogenesis in the developing chick

Hemming¹,

Saxod²

1998

Histochemistry and Cell Biology

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Keratan sulphate proteoglycans are potentially important during development and are possible binding molecules for the lectin, peanut agglutinin, a marker for areas that are inhibitory for axonal growth in early embryos. The present study describes the spatiotemporal distributions of keratan sulphate epitopes and peanut agglutinin binding sites during organogenesis in the developing chick from E5 to hatching. The widespread distributions of these molecules did not often overlap but clearly delimited different carbohydrate compartments demonstrating that peanut agglutinin does not necessarily bind to keratan sulphate proteoglycans. These markers were mostly extracellular but keratan sulphate, in particular, was found within certain specific cells in cartilage, gonad, heart and pancreas, at certain ages. The presence of keratan sulphate in putative germ cells during their migrations and in the gonads may be of particular importance. Their distributions generally evoke modulation of adhesion allowing cell migrations or morphogenetic movements related to epitheliomesenchymal interactions, but may also suggest an involvement in axonal guidance in skin, cartilage, gut and possibly heart. Furthermore, in the kidney, peanut agglutinin binding sites seem to be related to the functional differentiation of the nephrons.

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Developmental expression of keratan sulfate-like immunoreactivity distinguishes thalamic nuclei and cortical domains

Cited by 25 publications

References 78 publications

KSGal6ST Is Essential for the 6-Sulfation of Galactose within Keratan Sulfate in Early Postnatal Brain

KSGal6ST Is Essential for the 6-Sulfation of Galactose within Keratan Sulfate in Early Postnatal Brain

Time‐dependent localization of high‐ and low‐sulfated keratan sulfates in the song nuclei of developing zebra finches

Regulated expression of keratan sulphate and peanut agglutinin binding sites during organogenesis in the developing chick

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