Developmental expression of the glutathione S‐transferase Yo subunit in the rat testis and epididymis using light microscope immunocytochemistry

Papp, Steve; Robaire, Bernard; Hermo, Louis

doi:10.1002/ar.1092400307

Cited by 19 publications

(13 citation statements)

References 36 publications

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“…Our results show that rat testes are also rich in GST activity (Fig. 6), confirming studies by others [Kraus and Kloft, 1980;Guthenberg et al, 1983;Di Biasio et al, 1991;Papp et al, 1994;Hermo et al, 1994]. Furthermore, in control rats the testis showed significantly higher GST activity than the liver during the entire length of the study, i.e., 20 weeks (Fig.…”

Section: Discussionsupporting

confidence: 95%

Temporal patterns of DNA adduct formation and glutathione S-transferase activity in the testes of rats fed aflatoxin B1: A comparison with patterns in the liver

Sotomayor

Sahu

Washington

et al. 1999

Environ. Mol. Mutagen.

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Section: Discussionsupporting

confidence: 95%

Temporal patterns of DNA adduct formation and glutathione S-transferase activity in the testes of rats fed aflatoxin B1: A comparison with patterns in the liver

Sotomayor

Sahu

Washington

et al. 1999

Environ. Mol. Mutagen.

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“…Indeed, the first appearance of a significant increase in SGP-1 endosomal labeling occurs by day 39. Recently it has been shown that spermatozoa are already released from the testis by day 39 as evidenced by the presence of residual bodies bordering the lumen at this age (Papp et al, 1994). Although the tubules are still small in comparison to those at days 49 and 56 and the number of spermatozoa still low, this study indicates that spermiation occurs as early as day 39 (Papp et al, 1994) and not as late as day 42 as suggested by some investigators (Knorr et al, 1970).…”

Section: Sgp-1 Labeling Content In Nonciliated Cellssupporting

confidence: 75%

“…Thus similarly to SGP-1, it appears that SGP-2 endosomal labeling may be dependent on the entry of spermatozoa into the lumen of the efferent ducts occurring at this age (Papp et al, 1994). Interestingly, the SGP-2 lysosomal labeling content increased sharply by day 29 despite insignificant levels of endosomal labeling at this age.…”

Section: Sgp-2 Labeling In Nonciliated Cells Duringmentioning

confidence: 85%

“…Recently it has been shown that spermatozoa are already released from the testis by day 39 as evidenced by the presence of residual bodies bordering the lumen at this age (Papp et al, 1994). Although the tubules are still small in comparison to those at days 49 and 56 and the number of spermatozoa still low, this study indicates that spermiation occurs as early as day 39 (Papp et al, 1994) and not as late as day 42 as suggested by some investigators (Knorr et al, 1970). Considering on the one hand that the lumen of seminiferous tubules becomes patent at about day 18 (Vitale et al, 1973;Tindall et al, 1975) while that of the efferent ducts is already patent by day 15 (Papp et al, 1994) and on the other that mRNA transcripts for SGP-1 in Sertoli cells are already present by day 21 (Collard et al, 19881, it would appear that endocytosis of the Sertoliderived SGP-1 by nonciliated cells coincides with the entry of spermatozoa into the lumen of the efferent ducts by day 39.…”

Section: Sgp-1 Labeling Content In Nonciliated Cellsmentioning

confidence: 99%

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Targeting of endogenous sulfated glycoprotein‐1 and ‐2 to lysosomes within nonciliated cells of the efferent ducts during postnatal development of the rat

Hermo

Rosenthal

Igdoura

et al. 1995

Molecular Reproduction Devel

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Sulfated glycoprotein (SGP)-1 and -2, secretory products of Sertoli cells, are secreted into the lumen of seminiferous tubules where they bind to late spermatids. Once released, the spermatozoa traverse the efferent ducts where these proteins detach from their surface and are endocytosed by the nonciliated cells. In adult animals, SGP-1 and SGP-2 are also synthesized by nonciliated cells and targeted from the Golgi apparatus to lysosomes. The purpose of the present study was to determine the pattern of expression of SGP-1 and SGP-2 within nonciliated cells during postnatal development. The efferent ducts of animals at different postnatal ages were prepared for an electron microscopic immunocytochemical quantitative analysis as well as for Northern blot analysis. The data expressed as labeling content (no. gold particles/micron 2 and taking into account the volume of the endocytic organelles and the cell) revealed that anti-SGP-1 labeling in endosomes of nonciliated cells was minimal at 15, 21, and 29 days of age. On the other hand, the lysosomal labeling content showed a significant increase by day 29 compared to 15 and 21-day-old animals indicating that an endogenous form of SGP-1 was being synthesized by nonciliated cells and targeted to lysosomes. By day 39 a significant increase in endosomal labeling occurred; this was attributed to the endocytosis of Sertoli-derived SGP-1 which coincided with the entry of spermatozoa into the lumen of these ducts at this age. Lysosomal labeling showed further significant increases at days 39, 49, and then again at day 90. Northern blot analysis detected SGP-1 mRNA transcripts at all postnatal ages examined. While decreases or increases in transcripts could not be determined due to the greater amount of tissue present with increasing age, these data taken together support the idea of an endogenous form of SGP-1 being synthesized by nonciliated cells and targeted to lysosomes during postnatal development. In the case of SGP-2, endosomal labeling was minimal at 15, 21, and 29 days of age but was significantly increased by day 39, with similar values at all subsequent ages. The high value at day 39 was attributed to the endocytosis of SGP-2 which coincided with the entry of spermatozoa into the lumen at this age. Lysosomal labeling, on the other hand, was low at days 15 and 21 but peaked at day 29 at a time when endosomal labeling was minimal. These results suggested the synthesis of an endogenous form of SGP-2 which was being targeted to lysosomes.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…GST activity has been demonstrated at several levels in the male genital tract: epididymal cells (Robaire and Viger, 1995), in pachytene spermatocytes, spermatids and cytoplasmic droplets (Yoganathan et al, 1989;Papp et al, 1994;Veri et al, 1994) and the g-class of GST has been detected in mouse and rat spermatozoa (Aravinda et al, 1995;Fulcher et al, 1995). In the rat, GST was detected immunocytochemically on sperm acrosome (Aravinda et al, 1995).…”

Section: Discussionmentioning

confidence: 99%

Effect of indole-3-acetic acid (plant auxin) on the preservation at 15 °C of boar semen for artificial insemination

Toniolli¹,

Bussière²,

Courot³

et al. 1996

Reprod. Nutr. Dev.

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Summary― In order to extend the duration of boar sperm survival at 15 °C for artificial insemination, we tested the effect of indole-3-acetic acid (IAA), which appeared to be the main sperm protective substance present in the Coco nucifera endosperm (coconut water). Two IAA concentrations (10 and 100 ng/mL) in Beltsville extender (BTS) were studied for their in vitro effects. The motility, the percentage of motile spermatozoa and the acrosome morphology of sperm were recorded each day over 13 days of storage at 15 °C, after 5 min and 3 h of incubation at 39 °C. The IAA effect on sperm preservation was also studied in vivo at a concentration of 10 ng/mL in BTS by inseminating groups of females twice at 24 h intervals either at DO (day of sperm collection) and D1 (DO/1) or at D5 and D6 (D5/6). At DO/1, the two groups of females (control and IAA) were inseminated with a total of 6.3 x 109 spermatozoa (3.15 x 10 9 at DO and the same dose at D1) whereas at D5/6, one IAA group was inseminated with a total of 6.3 x 10 9 spermatozoa and another one with 12.6 x 10 9 spermatozoa. The animals in the D5/6 control group were inseminated each with a total of 12.6 x 10 9 spermatozoa. For each group of females (n= 106-140), fertility rate (% farrowing) and prolificacy rate (litter size) were recorded. No effect of IAA in vitro on the motility rate and on the percentage of motile spermatozoa was observed over a 13 day storage. However, IAA (10 ng/mL) had a significant positive effect on the percentage of living spermatozoa with intact acrosomes after 13 days (66 vs 54%, P < 0.05). The fertility and prolificacy rates after 5-6 days of sperm preservation in BTS extender alone did not differ significantly between D5/6 and DO/1 but the total number of inseminated spermatozoa was 12.6 x 10 9 at D5/6 instead of 6.3 x 10 9 at DO/1. When the spermatozoa were stored in the presence of 10 ng/mL IAA for 5-6 days at 15 °C, the fertility and prolificacy of the females inseminated with only 6.3 x 10 9 spermatozoa were identical to those of the females inseminated with an equal number of spermatozoa at DO/1 in the presence or absence of IAA.pig I sperm / 3-indole-acetic acid I fertility I prolificacy I artificial insemination * Recipient of a fellowship from CNPq 70750-560 Brasilia, Brazil, on leave from the Faculdade de Veterinaria UECE 60715-000 Fortaleza Ceari, Brazil.

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Developmental expression of the glutathione S‐transferase Yo subunit in the rat testis and epididymis using light microscope immunocytochemistry

Abstract: Overall these results suggest that the expression of GST-Yo in the various cells of the testis and epididymis are controlled by different factors during postnatal development.

Cited by 19 publications

References 36 publications

Temporal patterns of DNA adduct formation and glutathione S-transferase activity in the testes of rats fed aflatoxin B1: A comparison with patterns in the liver

Temporal patterns of DNA adduct formation and glutathione S-transferase activity in the testes of rats fed aflatoxin B1: A comparison with patterns in the liver

Targeting of endogenous sulfated glycoprotein‐1 and ‐2 to lysosomes within nonciliated cells of the efferent ducts during postnatal development of the rat

Effect of indole-3-acetic acid (plant auxin) on the preservation at 15 °C of boar semen for artificial insemination

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