Developmental expression of trehalase: role of transcriptional activation

Gärtner, Hans; Shukla, Pramila; Markesich, Diane C.; Solomon, Nitikul S; Oesterreicher, Thomas J.; Henning, Susan J.

doi:10.1016/s0167-4781(02)00231-2

Cited by 14 publications

(22 citation statements)

References 16 publications

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“…We found that the gene expressions of Si and Treh, belonging to the disaccharidase family, increased rapidly from postnatal days 13 to 27. These results are consistent with previous reports (2,3,5). In addition, several studies have shown that the expressions of hexose transporter genes are induced during this transient period (4,13).…”

Section: Discussionsupporting

confidence: 93%

“…In addition, HNF-4 enhances the expression of intestinal genes, such as apolipoprotein A-IV (30) and guanylyl cyclase (31), although these genes are not listed among the upregulated/downregulated genes. It is reported not only that glucocorticoid hormone enhanced intestinal genes such as Si, Slc2a5 and Treh (5,13,32), but also that GR directly regulated Slc2a5 expression (13,33). PPARGC1A may enhance intestinal gene expressions by activating transcriptional factors such as CREB, HNF-4 and GR.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, it was reported that endogenous stimulation by thyroid hormones and glucocorticoid hormones in suckling rats induces intestinal maturation (50). Furthermore, it is known that glucocorticoid hormones (Si, Slc2a5 and Treh) and thyroid hormones (Si, Slc2a5 and Treh) enhance intestinal gene expressions during the transient suckling-weaning period (4,5,13,32). In addition, the expressions of these genes are known to be upregulated by dietary carbohydrate (9,10,51,52).…”

Section: Discussionmentioning

confidence: 99%

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Gene Expression Changes in the Jejunum of Rats during the Transient Suckling-Weaning Period

Mochizuki

Yorita

Goda

2009

J Nutr Sci Vitaminol

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SummaryIt is well-known that the small intestine of rodents rapidly undergoes differentiation and maturation during the transient suckling-weaning period from postnatal days 13 to 27. In the present study, we examined the gene expression changes in the jejunum of rats during the transient suckling-weaning period by microarray analysis. In the microarray data, we found that the expressions of many genes related to digestion/absorption/ excretion of nutrients/ions, such as members of the solute carrier ( Slc ) family and ATP-binding cassette ( Abc ) subfamily, were rapidly induced during this period. Furthermore, some transcriptional factors/cofactors ( Thrsp , Ppargc1a , Klf15 and Vdr ), which are presumably important for the induction of intestinal gene expression after weaning, were rapidly induced during this period. In contrast, genes related to transport of nutrients, such as folate, zinc, fat and phosphate, which are important for early development, were highly expressed in the suckling period and then gradually decreased during weaning. These results indicate that the jejunum matures during the suckling-weaning period accompanied by changes in the expression of many genes related to digestion/absorption/excretion and some genes for transcriptional factors/cofactors. Key Words transient suckling-weaning period, jejunum, solute carrier family, ATP-binding cassette subfamily, transcriptional factors/cofactorsThe morphology of the small intestine in rodents changes dramatically during the first 2-3 wk after birth as they are gradually weaned. During this period, the diet composition changes from one with less carbohydrate (milk) to one rich in carbohydrate (solid food) ( 1 ). Several studies have already shown that the expressions of genes related to carbohydrate digestion/absorption, such as disaccharidases [sucrase-isomaltase ( Si ) and trehalase ( Treh )], which are involved in the digestion of starch/sucrose or trehalose into monosaccharides, and hexose transporters [SGLT1 ( Slc5a1 ), GLUT5 ( Slc2a5 ) and GLUT2 ( Slc2a2 )], which are involved in monosaccharide absorption from the lumen, are elevated between 2 and 3 wk after birth in rodents ( 2 -5 ). In addition, we previously reported that the gene expressions of liver-type fatty acid-binding protein [L-FABP ( Fabp1 )] and cellular retinol-binding protein type II [CRBPII ( Rbp2 )], which are involved in transporting fatty acids and vitamin A, respectively, from the lumen to enterocytes, as well as a ␤ -oxidation rate-controlling gene [acyl-CoA oxidase ( Acox1 )], were highly expressed in the transient suckling-weaning period, and declined after weaning ( 6 , 7 ). Furthermore, the gene expression of peroxisome proliferator-activated receptor ␣ [PPAR ␣ ( Ppara )], a transcriptional factor for these genes, was associated with these gene expression changes ( 6 ).These changes during the transient suckling-weaning period may be regulated by the nutritional changes as well as hormones, such as thyroid hormone and glucocorticoid hormone, because such hormones ar...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Gene Expression Changes in the Jejunum of Rats during the Transient Suckling-Weaning Period

Mochizuki

Yorita

Goda

2009

J Nutr Sci Vitaminol

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“…In addition, small-intestinal hyperplasia, and the resultant increase of total activity of disaccharidases such as sucrase and isomaltase in the entire small intestine were noted, thereby leading to postprandial hyperglycaemia in diabetes mellitus (54) . Concerning normal development of the GIT, studies of the ontogeny of intestinal enzymes (for example, trehalase, see Gartner et al (55) ) and hormones in rodents suggest that epigenetic mechanisms of gene regulation in the intestine could be responsible for the continuity of maturation during postnatal growth. Direct evidence of the implication of epigenetic mechanisms in GIT pathology are provided by several examples in which an epigenetic deregulation is the cause of carcinogenesis (50) .…”

Section: Effects Of Nutritional Programming On the Gastrointestinal Tmentioning

confidence: 99%

Nutritional programming of gastrointestinal tract development. Is the pig a good model for man?

Guilloteau

Zabielski²,

Hammon³

et al. 2010

Nutr. Res. Rev.

271

222

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The consequences of early-life nutritional programming in man and other mammalian species have been studied chiefly at the metabolic level. Very few studies, if any, have been performed in the gastrointestinal tract (GIT) as the target organ, but extensive GIT studies are needed since the GIT plays a key role in nutrient supply and has an impact on functions of the entire organism. The possible deleterious effects of nutritional programming at the metabolic level were discovered following epidemiological studies in human subjects, and confirmed in animal models. Investigating the impact of programming on GIT structure and function would need appropriate animal models due to ethical restrictions in the use of human subjects. The aim of the present review is to discuss the use of pigs as an animal model as a compromise between ethically acceptable animal studies and the requirement of data which can be interpolated to the human situation. In nutritional programming studies, rodents are the most frequently used model for man, but GIT development and digestive function in rodents are considerably different from those in man. In that aspect, the pig GIT is much closer to the human than that of rodents. The swine species is closely comparable with man in many nutritional and digestive aspects, and thus provides ample opportunity to be used in investigations on the consequences of nutritional programming for the GIT. In particular, the 'sow -piglets' dyad could be a useful tool to simulate the 'human mother-infant' dyad in studies which examine short-, middle-and long-term effects and is suggested as the reference model.

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“…For sucrase-isomaltase and trehalase, further studies (13,47) have indicated that the effect of glucocorticoid on mRNA levels in turn reflects activation of transcription. On the basis of the classical model of glucocorticoid action (10), the simplest mechanism to explain these findings would be one in which the glucocorticoid receptor functioned as a transcriptional activator of these genes.…”

mentioning

confidence: 99%

Immediate early genes of glucocorticoid action on the developing intestine

Agbemafle¹,

Oesterreicher²,

Shaw³

et al. 2005

American Journal of Physiology-Gastrointestinal and Liver Physi

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Prior studies have demonstrated that glucocorticoid hormones elicit functional maturation of the small intestine as evidenced by their ability to induce increases in the expression of various digestive hydrolases, such as sucrase-isomaltase and trehalase. However, these increases have a lag time of ∼24 h, suggesting that they are secondary effects of hormone action. To identify candidate primary response genes, we performed microarray analysis on pooled RNA from jejunums of untreated postnatal day 8 mouse pups and from littermates who earlier received dexamethasone 2 h. Fluorescent dye-labeled samples were hybridized in quadruplicate to glass-spotted cDNA microarrays containing 15,000 cDNA clones from the National Institute of Aging cDNA clone set. Analysis of the resulting signals using relatively stringent criteria identified 66 transcripts upregulated and 36 downregulated by 2 h of glucocorticoid treatment. Among the upregulated transcripts, the magnitude of the increase detected by microarray ranged from 1.4- to 16-fold. Selected mRNAs from throughout the range were subsequently analyzed by Northern blot analysis. Of 11 mRNAs chosen all were confirmed, and there was a strong correlation between the magnitude of the increase observed from the microarray analysis and from Northern blot analysis. Additional time points showed that these transcripts peaked between 2 and 6 h and had returned to baseline by 24 h. Gene ontology analysis showed pleiotropic effects of dexamethasone on the developing intestine and pointed to genes in the development category as being likely candidates for mediation of glucocorticoid-induced maturation of intestinal function.

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Developmental expression of trehalase: role of transcriptional activation

Cited by 14 publications

References 16 publications

Gene Expression Changes in the Jejunum of Rats during the Transient Suckling-Weaning Period

Gene Expression Changes in the Jejunum of Rats during the Transient Suckling-Weaning Period

Nutritional programming of gastrointestinal tract development. Is the pig a good model for man?

Immediate early genes of glucocorticoid action on the developing intestine

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