Developmental hematopoiesis in normal human fetal blood

Forestier, F.; Daffos, F.; Catherine, Nicole; Renard, Michèle; Andreux, J.‐P.

doi:10.1182/blood.v77.11.2360.2360

Cited by 199 publications

(57 citation statements)

References 29 publications

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“…In 18/30 (60%) cases, erythroblastosis was present according to gestational age corrected reference ranges. 17 Leucocyte and neutrophil counts were all normal when compared with gestational age adjusted reference values. 17…”

Section: Haemoglobin and Leucocyte Countsmentioning

confidence: 87%

“…The factor 2 is used in the numerator of the equation to allow for possible platelet sequestration in the spleen or liver. 6 Since both fetal haemoglobin concentrations and platelet counts vary with gestational age, reference ranges published by Forestier et al 17 were used. These reference ranges were used to be able to compare our results with those of Schild's group.…”

Section: Clinical Datamentioning

confidence: 99%

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Thrombocytopenia in hydropic fetuses with parvovirus B19 infection: incidence, treatment and correlation with fetal B19 viral load

Haan

Akker

Porcelijn

et al. 2007

BJOG

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Objective To examine (1) the incidence of fetal thrombocytopenia in hydropic fetuses with congenital B19 virus infection, (2) the effect of intrauterine platelet transfusions and (3) the correlation between fetal B19 viral load and severity of thrombocytopenia.Design Retrospective analysis of data from prospectively collected fetal blood samples.Setting Leiden University Medical Centre, the national centre for management of intrauterine fetal disease in the Netherlands.Population Thirty hydropic fetuses treated with intrauterine red blood cell and platelet transfusions for human B19 virus-induced severe fetal anaemia and thrombocytopenia over a 10-year period.Methods Fetal blood samples (n = 30) taken before and after intrauterine transfusion were investigated. No cases were excluded, and there was no loss to follow up.Main outcome measures Parameters recorded were gestational age, experienced fetal movements, gravidity and parity, severity of fetal hydrops, severity of fetal anaemia and thrombocytopenia and megakaryocyte and reticulocyte counts. Survival and procedureassociated complications were documented. Quantitative B19 viral load measurements were performed on all fetal samples.Results Forty-six percent of all hydropic fetuses showed severe thrombocytopenia. No antenatal intracerebral haemorrhage or procedure-associated bleeding occurred. Overall, survival was 77%. Platelet counts increased following platelet transfusion and decreased significantly following red blood cell transfusion alone. No correlation was found between fetal viral loads and platelet counts.Conclusion Thrombocytopenia was frequently encountered in fetal B19V infection, but fetal bleeding complications were not noted. Absence of a direct relationship between fetal B19 viral load and platelet counts suggests a temporal dissociation between these findings. Dilutional thrombocytopenia is frequently seen in the fetus following red blood cell transfusion alone. The clinical significance of this phenomenon is unclear. The risk of fluid overload by fetal platelet transfusion in a severely hydropic fetus should be weighed against the low incidence of fetal bleeding complications.

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Section: Haemoglobin and Leucocyte Countsmentioning

confidence: 87%

Section: Clinical Datamentioning

confidence: 99%

Thrombocytopenia in hydropic fetuses with parvovirus B19 infection: incidence, treatment and correlation with fetal B19 viral load

Haan

Akker

Porcelijn

et al. 2007

BJOG

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“…8,9 However, < 10% of leucocytes in fetal blood are neutrophils between weeks 18 and 30, whereas 85-88% are lymphocytes and about 3% are monocytes. 10 Moreover, lymphocytes populate primary and secondary lymphoid organs early during fetal development. 11 T-lymphocyte precursors colonize thymus at weeks 7-8, mature T cells are found in the periphery at weeks 10-12, and B cells in liver at week 9.…”

Section: Introductionmentioning

confidence: 99%

Expression and function of endothelial selectins during human development

2014

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SummaryLeucocyte trafficking is vital for the immune defence. In adults, early tethering and rolling interactions between leucocytes and endothelial cells are mediated by P-, E-and L-selectins and their ligands. In contrast, the role of selectins in migration of mononuclear cells during fetal development in humans remains unknown. We studied the functions of endothelial Eand P-selectins and their counter-receptors during human ontogeny. Immunohistochemical stainings showed that P-selectin is expressed in megakaryocytes and endothelial cells starting from gestational weeks 7 and 11, respectively. Endothelial E-selectin appeared latest, at week 32. Real-time imaging using in vitro flow chamber assays showed that cord blood mononuclear leucocytes used E-, P-and L-selectin and PSGL-1 to roll on and adhere to endothelium under physiological shear stress. These data show that selectins are synthesized and functional before birth in humans and have the potential to mediate the emigration of mononuclear cells and inflammatory responses.

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“…Moreover, after umbilical cord milking the hemoglobin level of 12.9 ± 1.4 g/dL in infants born between 26 and 29 weeks of gestation was not high enough to produce polycythemia. 17 Moreover, no infants with polycythemia were observed among the extremely low-birthweight infants in our previous study, 12 or in the most recent meta-analysis. 2 No differences in any parameters between the vaginal delivery group and the cesarean section group were seen on statistical analysis.…”

Section: Discussionmentioning

confidence: 71%