Developmental patterns of mST3GaIV mRNA expression in the mouse:In situ hybridization using DIG-labeled RNA probes

Ji, Min; Lee, Young Choon; Il, Su; Nam, Sang Yoon; Jung, Kyu Yong; Kim, Hyoung Min; Park, Sang-Youel; Choo, Young‐Kug

doi:10.1007/bf02976584

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…Ganglioside GM3 was synthesized by mST3GalV, and the expression and regulation of mST3GalV (CMP-NeuAc: lactosylceramide alpha-2, 3-sialyltransferase) activity is central to the production of almost all gangliosides. Spatial and temporal expression of mST3GalV mRNA (GM3) during mouse embryogenesis [on embryonic (E) days E9, E11, E13, and E15] was demonstrated by in situ hybridization with digoxigenin-labeled RNA probes (Ji et al, 2000, Table 1). All tissue samples obtained on E9 and E11 were observed to have the same level of mST3GalV mRNA expression.…”

Section: Gangliosides In Mouse Embryonic Developmentmentioning

confidence: 99%

“…All tissue samples obtained on E9 and E11 were observed to have the same level of mST3GalV mRNA expression. On E13, mST3GalV mRNA was expressed in various neural and non-neural tissues and in the telencephalon, while on E15, strong expression of mST3Gal V was observed in the liver (Ji et al, 2000). Bouvier and Seyfried (1989) observed that the predominant gangliosides in E12 mouse embryos were GD3 (51% of total sialic distribution), GM3 (19%), and GT1b (9.6%); other gangliosides occurred in much lower amounts (GM2 (2.6%), GM1 (1.6%), GD1a (3.7%), GD1b (6.3%), and GQ1b (4.5%)).…”

Section: Gangliosides In Mouse Embryonic Developmentmentioning

confidence: 99%

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Roles of gangliosides in mouse embryogenesis and embryonic stem cell differentiation

et al. 2011

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Gangliosides have been suggested to play important roles in various functions such as adhesion, cell differentiation, growth control, and signaling. Mouse follicular development, ovulation, and luteinization during the estrous cycle are regulated by several hormones and cell-cell interactions. In addition, spermatogenesis in seminiferous tubules of adult testes is also regulated by several hormones, including follicle-stimulating hormone (FSH) and luteinizing hormone (LH) and cell-cell interactions. The regulation of these processes by hormones and cell-cell interactions provides evidence for the importance of surface membrane components, including gangliosides. During preimplantation embryo development, a mammalian embryo undergoes a series of cleavage divisions whereby a zygote is converted into a blastocyst that is sufficiently competent to be implanted in the maternal uterus and continue its development. Mouse embryonic stem (mES) cells are pluripotent cells derived from mouse embryo, specifically, from the inner cell mass of blastocysts. Differentiated neuronal cells are derived from mES cells through the formation of embryonic bodies (EBs). EBs recapitulate many aspects of lineage-specific differentiation and temporal and spatial gene expression patterns during early embryogenesis. Previous studies on ganglioside expression during mouse embryonic development (including during in vitro fertilization, ovulation, spermatogenesis, and embryogenesis) reported that gangliosides were expressed in both undifferentiated and differentiated (or differentiating) mES cells. In this review, we summarize some of the advances in our understanding of the functional roles of gangliosides during the stages of mouse embryonic development, including ovulation, spermatogenesis, and embryogenesis, focusing on undifferentiated and differentiated mES cells (neuronal cells).

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Section: Gangliosides In Mouse Embryonic Developmentmentioning

confidence: 99%

Section: Gangliosides In Mouse Embryonic Developmentmentioning

confidence: 99%

Roles of gangliosides in mouse embryogenesis and embryonic stem cell differentiation

et al. 2011

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“…These gangliosides are thought to be involved in the control of several biological processes, including apoptosis, mouse embryonic development, cell proliferation, cell surface interactions, cell differentiation, and transmembrane signaling (10,11). GM3 is mostly expressed during embryogenesis in mice (12,13), but its expression is decreased during brain development (14). GD1a is also expressed during late embryogenesis (E9-E16), and increased expression of GD1a is observed during mouse brain development (12,14).…”

Section: Introductionmentioning

confidence: 99%

“…GM3 is mostly expressed during embryogenesis in mice (12,13), but its expression is decreased during brain development (14). GD1a is also expressed during late embryogenesis (E9-E16), and increased expression of GD1a is observed during mouse brain development (12,14). In particular, GM1 has been shown to possess antineurotoxic, neuroprotective, and neurorestorative capabilities, as well as the ability to facilitate neuronal commitment (15,16).…”

Section: Introductionmentioning

confidence: 99%

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

Ryu

Chang²,

Lee³

et al. 2012

BMB Reports

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Gangliosides play important roles in the control of several biological processes, including proliferation and transmembrane signaling. In this study, we demonstrate the effect of ganglioside GM1 on the proliferation of mouse induced pluripotent stem cells (miPSCs). The proliferation rate of miPSCs was lower than in mouse embryonic stem cells (mESCs). Fluorescence activated cell sorting analysis showed that the percentage of cells in the G2/M phase in miPSCs was lower than that in mESCs. GM1 was expressed in mESCs, but not miPSCs. To confirm the role of GM1 in miPSC proliferation, miPSCs were treated with GM1. GM1-treated miPSCs exhibited increased cell proliferation and a larger number of cells in the G2/M phase. Furthermore, phosphorylation of mitogen-activated protein kinases was increased in GM1-treated miPSCs. [BMB Reports 2012; 45(12): 713-718]

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“…Sialyated glycosphingolipids from the ganglio-series are normally classified into four series from 0-to c-series and shed actively into the tumor microenviroment (Svennerholm 1980;Kudo et al 2003). Human normal tissues express various gangliosides of 0-and a-series (Ji et al 2000;Yamashita et al 1999) and its complex expression is increased under manifold pathological disorders (Ariga et al 2008), immune diseases (Shahrizaila and Yuki 2011), and cancer (Bobowski et al 2012). GD3 has been considered a cell death effector because of its ability to interact with mitochondria-mediated apoptosome activation and subsequent apoptosis based on death ligands (Paris et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Relationship between ganglioside expression and anti-cancer effects of a plant-derived antibody in breast cancer cells

Song

Park

et al. 2019

J Plant Biotechnol

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Production of therapeutic monoclonal antibodies (mAbs) using a plant platform has been considered an alternative to the mammalian cell-based production system. A plant-derived mAb CO17-1AK (mAb P COK) can specifically bind to various types of cancer cell lines. The target protein of mAb P COK is the epithelial cell adhesion molecule (EpCAM) highly expressed in human epithelial cancer cells, including breast and colorectal cancer cells. It has been hypothesized that its overexpression supports tumor growth and metastasis. A ganglioside is extended well beyond the surfaces of the various cell membranes and has roles in cell growth, inflammation, differentiation, and carcinogenesis. However, the regulation of EpCAM gene expression in breast cancers and the role of gangliosides in oncogenesis are unclear. Here, the purpose of this study was to determine the effects of mAb P COK on human breast cancer cell proliferation, apoptosis, and ganglioside expression patterns. Our results show that treatment with mAb P COK suppressed the growth of breast cancer cells and induced apoptotic cell death. It also upregulated the expression of metastasis-related gangliosides in breast cancer cells. Thus, treatment with mAb P COK may have chemo-preventive therapeutic effects against human breast cancer.

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Developmental patterns of mST3GaIV mRNA expression in the mouse:In situ hybridization using DIG-labeled RNA probes

Cited by 8 publications

References 26 publications

Roles of gangliosides in mouse embryogenesis and embryonic stem cell differentiation

Roles of gangliosides in mouse embryogenesis and embryonic stem cell differentiation

Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

Relationship between ganglioside expression and anti-cancer effects of a plant-derived antibody in breast cancer cells

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