Developmental profile of select immune cells in mice infected with Trichinella spiralis during the intestinal phase

Ding, Jin; Bai, Xue; Wang, X.L.; Wang, Yanfei; Shi, Hailian; Rosenthal, Benjamin M.; Boireau, Pascal; Wu, Xiuping; Liu, M.Y.; Liu, X.L.

doi:10.1016/j.vetpar.2016.07.019

Cited by 27 publications

(38 citation statements)

References 24 publications

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“…This finding was probably due to the rise of CD4+ T cells in all infection groups and CD8+ T cells in the high-dose group. CD4+ T cells are generated in the intestinal phase of T. spiralis after 2-4 dpi and play a key role in the immune response [43,44]. Our results showed that CD4+ T cells increased after infection and peaked at 3 dpi, which was similar to the results obtained by previous studies [43,44].…”

Section: As the Lowest Infection Dose In Pigssupporting

confidence: 91%

“…However, the number of CD8+ T cells was increased in the highest-dose infection group after infection, possibly because large numbers of worms cause severe damage and inflammation in the intestinal and muscle tissues, which triggers an expulsion worm response. It has been reported that both CD4+ and CD8+ T cells were increased in infected mice with 500 ML, and the levels of CD8+ T cells were increased higher than CD4+ T cells and with a declining ratio of CD4+/CD8+ T cells [43]. In our study, the ratio of CD4+/CD8+ T cells was elevated at the intestinal phase, which meant that the most serious suppression of the immune response occurred during the intestinal phase of pigs infected with T. spiralis.…”

Section: As the Lowest Infection Dose In Pigsmentioning

confidence: 92%

“…Antibodies were the key component of humoral immunity produced by B cells. The IgG antibodies can kill the NBL by induced eosinophils and ADCC [43]. A previous study demonstrated that the number of B cells significantly increased at 15 dpi in mice infected with T. spiralis [43].…”

Section: As the Lowest Infection Dose In Pigsmentioning

confidence: 94%

“…CD4+ T cells are generated in the intestinal phase of T. spiralis after 2-4 dpi and play a key role in the immune response [43,44]. Our results showed that CD4+ T cells increased after infection and peaked at 3 dpi, which was similar to the results obtained by previous studies [43,44]. Diarrhea was observed in humans at the intestinal phase of the infection due to inflammation caused by adult worms migrating to Peyer's patches and development and continuous antigen stimulation exposure to the host [45].…”

Section: As the Lowest Infection Dose In Pigsmentioning

confidence: 99%

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Primary characterization of the immune response in pigs infected with Trichinella spiralis

Wang

Bai

Tang

et al. 2020

Vet Res

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Trichinellosis, which is caused by Trichinella spiralis (T. spiralis), is a serious zoonosis. Pigs play an important role in the transmission of human trichinellosis. Characterizing the immune response to T. spiralis infection is key to elucidating host-parasite interactions. However, most studies on the immune response to T. spiralis infection have employed murine models. In this study, we investigated the immune response to T. spiralis infection in pigs. The results showed that the average numbers of larvae per gram (lpg) for the 100-muscle larvae (ML), 1000-ML, and 10 000-ML groups were 1.502, 35.947, and 398.811, respectively. The percentages of CD3+ T cells, B cells, CD4+ T cells, Treg cells, and Th17 cells were elevated in the infection groups compared to the control animals. In contrast, CD8+ T cell percentages were reduced after infection in the low-dose group. The number of neutrophils was increased at 3-17 days post-infection (dpi). Th1 cytokine IL-2 levels were significantly decreased at 7 dpi, and Th2 cytokine IL-4 levels were significantly elevated at 3 dpi. Treg cytokine IL-10 levels were significantly elevated between 7 dpi and 30 dpi. Th17 cytokine IL-17A levels were significantly increased beginning at 11 dpi. These results confirmed that pigs infected with T. spiralis predominantly induced Th2 and Treg immune responses, which suppress the Th1 immune responses. This study provides novel insights into the immune response of pigs infected with T. spiralis.

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Section: As the Lowest Infection Dose In Pigssupporting

confidence: 91%

Section: As the Lowest Infection Dose In Pigsmentioning

confidence: 92%

Section: As the Lowest Infection Dose In Pigsmentioning

confidence: 94%

Section: As the Lowest Infection Dose In Pigsmentioning

confidence: 99%

See 2 more Smart Citations

Primary characterization of the immune response in pigs infected with Trichinella spiralis

Wang

Bai

Tang

et al. 2020

Vet Res

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“…It has been demonstrated that mucosal IgA response was able to impede the establishment of Trichinella adult worms in the mouse intestine (Ding et al, 2016). Previous study has demonstrated that oral immunization with Ts Pmy/ S. typhimurium DNA vaccine induced Ts Pmy-specific sIgA secretion in intestinal mucosa possibly through inducing the expression of CCR9/CCR10, the homing receptors, on antibody secreting B cells and B cell translocation to local intestinal mucosa (Wang et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Heterologous Prime-Boost Vaccination Enhances TsPmy’s Protective Immunity against Trichinella spiralis Infection in a Murine Model

et al. 2017

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TsPmy is a paramyosin expressed by parasitic Trichinella spiralis and confers a protective immunity when its recombinant protein or DNA was used as an immunogen. To improve its immunogenicity and vaccine efficacy, we conducted a heterologous prime-boost strategy by orally delivering one dose of TsPmy DNA carried by attenuated Salmonella typhimurium (SL7207), followed by two doses of recombinant TsPmy intramuscularly. This strategy effectively induced intestinal mucosal sIgA response and an enhanced and balanced Th1/Th2 immune responses that improve protection against T. spiralis larval challenge, with 55.4% muscle larvae reduction and 41.8% adult worm reduction compared to PBS control. The muscle larvae reduction induced by heterologous prime-boost regimen was significant higher than that induced by the homologous DNA or protein prime-boost regimens, which could act as a practical prophylactic approach to prevent T. spiralis infection.

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Proteomics of Trichinella

Nagano

Khueangchiangkhwang

et al. 2021

Trichinella and Trichinellosis

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Developmental profile of select immune cells in mice infected with Trichinella spiralis during the intestinal phase

Cited by 27 publications

References 24 publications

Primary characterization of the immune response in pigs infected with Trichinella spiralis

Primary characterization of the immune response in pigs infected with Trichinella spiralis

Heterologous Prime-Boost Vaccination Enhances TsPmy’s Protective Immunity against Trichinella spiralis Infection in a Murine Model

Proteomics of Trichinella

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