Developmental profiles of gangliosides in mouse and rat cerebral cortex

Hilbig, Reinhard; Rösner, Harald; Merz, G.; Segler-Stahl, Kordula; Rahmann, H.

doi:10.1007/bf00848417

Cited by 48 publications

(21 citation statements)

References 30 publications

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“…Yavin and Yavin [11] reported that the increase in sialic acid and the developmental patterns of ganglioside spe cies in cultured rat cerebral cells agree with those in the developing prenatal brain. Similar observations have been made in other neuronal systems [12,13]. Dreyfus et al [10] proposed two discrete periods of changes in gan glioside concentration and pattern using cultured neu rons from 8-day-old chick embryonic brain.…”

Section: Discussionsupporting

confidence: 52%

“…In general, the period of synapse formation is marked by a decrease o f GD3 and an increase of G D la. The changes in ganglioside composition such as a decrease o f GD3 and an accretion of more complex gangliosides mimic developmental changes in embryonic rat and mouse brains [11][12][13], In the rat brain the change from synthesis of GM3 and GD3 to that of GM1 and G D la is reported to occur after embryonic day 18 [14].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Colchicine on Ganglioside Composition of Rat Primary Culture Neurons

Ogiso

Ohta²,

Harada³

et al. 1992

Dev Neurosci

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Developmental changes in gangliosides in the course of neurite outgrowth were examined in dissociated fetal rat cerebral neurons in culture. About a 2-fold increase in ganglioside levels was seen with the progression of neurite formation for up to 24 h in predominantly neuronal cultures. Ganglioside patterns appeared to be unchanged during the first 24 h, subsequently consisted of higher amounts of GD3 and b-series gangliosides (such as GD1b, GT1b, and GQ1b), and lower amounts of a-series gangliosides (GM1 and GD1a). Although the addition of colchicine to the cell growth medium inhibited neurite outgrowth in developing neurons, little if any differences in ganglioside patterns were found between control and colchicine-treated cells. Ganglioside levels decreased slightly in colchicine-treated cells in agreement with the decrease in cell attachment to culture dishes. Although colchicine treatment 8 h after plating caused complete retraction of formed neurites, the ganglioside level of the cells continued to increase during the following 16-hour incubation. Thus, the data suggest that ganglioside synthesis in differentiating neurons does not primarily accompany the expansion in cell surfaces due to neurite formation, and raises the possibility that a large proportion of gangliosides is retained in intracellular compartments.

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Section: Discussionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Effect of Colchicine on Ganglioside Composition of Rat Primary Culture Neurons

Ogiso

Ohta²,

Harada³

et al. 1992

Dev Neurosci

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“…We also studied the cell responsiveness to triiodothyronine by following its effect on in dexes such as cerebroside sulfotransferase (CST), an enzyme in oligodendroglia which is involved in the production of myelin sulfatides [73,[81][82][83], and gangliosides, which are particularly abundant in neuronal cells [18] and which show variations in their de velopmental pattern during cell maturation [8,29,32,54,55]. Preliminary accounts of these results have been published in abstract form [3,4,22],…”

Section: Introductionmentioning

confidence: 99%

Triiodothyronine Receptors in Developing Mouse Neuronal and Glial Cell Cultures and in Chick-Cultured Neurones and Astrocytes

et al. 1986

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The evolution of L-triiodothyronine (T₃) receptors was studied in developing cultures of cells dissociated from cerebral hemispheres of 14-day-old mouse embryos, which present successive distinct periods of cell proliferation and/or maturation. These periods are characterized essentially as neuronal from 1 to 12 days in vitro (DIV) and glial between 12 and 60 DIV. Furthermore myelin-related membranes are produced in this culture system. Binding capacities of the T₃ nuclear receptors increased from 1 to 6 DIV, when it reached a maximum (16 fmol/100 µg DNA). A similar increase of the DNA content of the cell was observed until 8 DIV. Thereafter a sharp fall of receptor concentration leading to a 5-fold decrease in the binding capacity occurred until day 15, a period at which neurones disappeared from the cultures. From 25 to 50 DIV (coinciding with the glial period), the concentration of receptor remained more or less constant (1–2 fmol/100 µg DNA). In parallel, the DNA content did not vary greatly between 30 and 50 DIV. Scatchard analysis revealed the presence of a single class of receptors at 6 and 20 DIV, representative of ''neuronal'' and ''glial'' periods, respectively. The equilibrium dissociation constant (Kd) of the nuclear receptor from cells at 6 DIV (2 x 10^–10M) was similar to that found at 20 DIV. These results were confirmed using pure cultured neurones and astrocytes prepared from embryonic chick brain. The effect of T₃ on the cellular gangliosides used as an index of neuronal cell maturation, and on cerebroside sulfotransferase (CST), an enzyme involved in the production of myelin sulfatides, was studied to determine a possible correlation between the binding capacity of the T₃ nuclear receptor and the response of the cultured cells to thyroid hormone. Our data demonstrate that T₃ had no significant effect either on the content of gangliosides or on their developmental pattern, while it increased the level of CST activity by 75% between 18 and 25 DIV. These results show that, although the concentration of T₃ receptors per 100 µg DNA in glial cells was lower than that in neurones, it was nevertheless sufficient to elicit a response in oligodendrocytes.

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“…Gangliosides are thought to play an im portant role in neuronal development be cause they are heavily concentrated in neu ronal membranes and undergo significant changes during neuronal differentiation and brain development [Ledeen, 1983;Fishman and Brady, 1976;Dreyfus et al, 1980;Irwin, 1979, 1982;Irwin et al, 1980;Landa and Moscona, 1985a, b;Yu et al, 1988;Rosner, 1980Rosner, , 1982Rosner et al, 1985;Yavin and Yavin, 1979;Engel et al, 1979;Hilbig et al, 1982;Seyfried et al, 1983Seyfried et al, , 1984. Before neuronal differentia tion, however, ganglioside composition is similar in neural and nonneural embryonic tissues [Bouvier and Seyfried, 1989].…”

Section: Discussionmentioning

confidence: 99%

Ganglioside GM1 Elevation in DBA/2 Mouse Embryos

Bouvier

Seyfried²

1990

Dev Neurosci

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The composition of whole embryo gangliosides at embryonic day E-12 was compared among the C57BL/6 (B6) DBA/2 (D2), and C3H mouse strains. N-acetylneuraminic acid was the predominant sialic acid species in the E-12 embryos. N-glycolyneuraminic acid was either undetectable or present in only trace amounts. Whole embryo ganglioside sialic concentration was significantly lower in D2 embryos than in B6 or C3H embryos. GM3 and GD3 were the most abundant ganglioside species in each strain and comprised approximately 75% of the total distribution. The D2 embryos expressed an elevation of GD1a and a reduction of GQ1b relative to B6 and C3H. Also, the level of GM1 was significantly higher in the D2 embryos than in the B6 or C3H embryos. Since a reduction of β-galactosidase activity and an elevation of GM1 concentration in brain were previously reported in postnatal DBA mice, our results suggest that the elevated GM1 in D2 embryos may result from a reduced activity of GM1 β-galactosidase.

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Developmental profiles of gangliosides in mouse and rat cerebral cortex

Cited by 48 publications

References 30 publications

Effect of Colchicine on Ganglioside Composition of Rat Primary Culture Neurons

Effect of Colchicine on Ganglioside Composition of Rat Primary Culture Neurons

Triiodothyronine Receptors in Developing Mouse Neuronal and Glial Cell Cultures and in Chick-Cultured Neurones and Astrocytes

Ganglioside GM1 Elevation in DBA/2 Mouse Embryos

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