Developmental Proteomic Profiling of Porcine Skeletal Muscle during Postnatal Development

Abstract

In this study, we have compared the skeletal muscle proteome at various stages of porcine postnatal development.Korean native pigs were divided into five postnatal stages of 30, 70, 130, 170 and 300 d and their loin muscles were analyzed for muscle proteome by using two-dimensional electrophoresis and mass spectrometry. We found 5 proteins showing a consistent pattern during skeletal muscle growth. Four proteins were identified as myosin light chain 1 slow-twitch (MLC1sa) isoform, troponin T, triosephosphate isomerase (TIP) and DJ-1 protein. The remaining protein was not identified. Two muscle fiber proteins of MLC1sa isoform and troponin T showed a high expression level at an early postnatal stage and then their levels were decreased markedly during growth stages. On the other hand, the expression of TIP and DJ-1 protein, which are well known as catalysis enzyme and antioxidantrelated protein, respectively, were linearly increased during growth stages. Thus, the stage-related muscle proteins may be useful as parameters for understanding the developmental characteristics of biochemical and physiological properties in Korean native pig skeletal muscle.

Animal board invited review: advances in proteomics for animal and food sciences

“…Although there has been more recent stimulus to the development of proteomics of animals in Europe, there have been valuable links with other continents and the science is evolving across the globe – for instance, in the Americas (Burgess, 2004 ; Lippolis and Reinhardt, 2008 ), Asia (Kim et al , 2007 ; Lu et al , 2012 ) and New Zealand (Plowman et al , 2007 ; Smolenski et al , 2007 ). One model example is the cooperative link established with New Zealand.…”

Integrative analysis of transcriptomics and proteomics of skeletal muscles of the Chinese indigenous Shaziling pig compared with the Yorkshire breed

“…Proteomic analysis based on two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) is a classical method in quantitative proteomics to separate mixtures of proteins into two dimensions and has been a powerful tool in meat science [ 23 , 24 ]. Many reports concerning differential proteomics among different pig breeds have been published [ 25 – 27 ]. Another technology for characterizing molecular changes in skeletal muscle is analysis of the transcriptome.…”

Differential proteome and transcriptome analysis of porcine skeletal muscle during development

“…The results showed up-regulation of DJ-1 during porcine skeletal muscle development, suggesting that DJ-1 may associate with the increasing resistance of muscle to oxidative stress in growth. Moreover, a previous research using proteome analysis also showed the quantitative increase of DJ-1 level during muscle growth [23]. The chaperonin TCP1 was essential for cells and plays a critically important role in the folding and biogenesis of many cytoskeletal proteins, including tubulin and actin [70], and TCP1 was especially highly expressed in 3 days newborn pigs.…”

“…The basic technologies for genome-wide expression analyses at the mRNA and protein levels have proven to be promising and powerful approaches in characterizing the changes in skeletal muscle biological processes [16][17][18]. In recent years, many transcriptome-or proteome-based studies have been successfully adopted for the analysis of the porcine skeletal muscle [7,[19][20][21][22][23][24][25]. But combining proteomic and transcriptional analyses of skeletal muscle is very limited and has been performed in human and mice [26][27][28].…”