Developmental regulation of hippocampal long-term depression by cofilin-mediated actin reorganization

Cao, Fan; Zhou, Zikai; Pan, Xingxiu; Leung, Celeste; Xie, Wei; Collingridge, Graham L.; Jia, Zhengping

doi:10.1016/j.neuropharm.2016.08.017

Cited by 16 publications

(12 citation statements)

References 61 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were seen in DHPG-induced LTD, where no effect was found either S3 or pS3 in young GluA2 KO mice (Figure 3D , S3: 59.4 ± 5.3%, n = 6; pS3: 72.3 ± 9.3%, n = 7; p > 0.05). These results show that, similar to WT animals (Cao et al, 2017 ), cofilin-mediated actin reorganization is not required for both LFS- and DHPG-induced LTD in young GluA2 KO mice.…”

Section: Resultssupporting

confidence: 63%

“…To test whether the PP-LFS induced LTD in mature mice was endocytosis dependent, we applied the endocytosis inhibitor Dynasore (80 μM) and found that LTD was blocked (Figure 5B , WT: 95.6 ± 10.1%, n = 5; p > 0.05 compared to baseline). These results are in clear contrast to mature WT animals where pep2m blocks LTD expression (Cao et al, 2017 ), suggesting that the requirement for NSF in adult GluA2 KO mice is also altered. We previously showed that the cofilin inhibitory peptide pS3 blocks LTD whereas the activation peptide S3 has no effects in mature WT mice (Cao et al, 2017 ).…”

Section: Resultsmentioning

confidence: 82%

“…This peptide has been shown to causes a run-down of AMPAR-mediated basal synaptic transmission (Nishimune et al, 1998 ; Song et al, 1998 ) and to prevent LTD in rats (Lüscher et al, 1999 ; Lüthi et al, 1999 ; Lee et al, 2002 ). We have previously shown that pep2m impairs both basal synaptic transmission and LTD expression in WT mice (Cao et al, 2017 ). Therefore, we tested the effect of pep2m in young GluA2 KO mice.…”

Section: Resultsmentioning

confidence: 95%

“…Actin filaments are the predominant cytoskeleton element in dendritic spines and their dynamic changes are important for spine morphology and synaptic plasticity (Cingolani and Goda, 2008 ). Our previous studies demonstrated that cofilin-mediated actin depolymerization is required for LTD expression (Zhou et al, 2011 ) and this requirement appears to be age-dependent (Cao et al, 2017 ). We first confirmed that under whole-cell recording mode without any inhibitor infusion, LFS induced an indistinguishable amount of LTD in WT and GluA2 KO mice (Figure 3A , WT: 53.9 ± 10.3%, n = 5; KO = 68.5 ± 9.4, n = 4, p > 0.05).…”

Section: Resultsmentioning

confidence: 99%

See 3 more Smart Citations

Hippocampal Long-Term Depression in the Presence of Calcium-Permeable AMPA Receptors

Cao

Zhou

Cai

et al. 2018

Front. Synaptic Neurosci.

Self Cite

View full text Add to dashboard Cite

The GluA2 subunit of AMPA glutamate receptors (AMPARs) has been shown to be critical for the expression of NMDA receptor (NMDAR)-dependent long-term depression (LTD). However, in young GluA2 knockout (KO) mice, this form of LTD can still be induced in the hippocampus, suggesting that LTD mechanisms may be modified in the presence of GluA2-lacking, Ca2+ permeable AMPARs. In this study, we examined LTD at the CA1 synapse in GluA2 KO mice by using several well-established inhibitory peptides known to block LTD in wild type (WT) rodents. We showed that while LTD in the KO mice is still blocked by the protein interacting with C kinase 1 (PICK1) peptide pepEVKI, it becomes insensitive to the N-ethylmaleimide-sensitive factor (NSF) peptide pep2m. In addition, the effects of actin and cofilin inhibitory peptides were also altered. These results indicate that in the absence of GluA2, LTD expression mechanisms are different from those in WT animals, suggesting that there are multiple molecular processes enabling LTD expression that are adaptable to physiological and genetic manipulations.

show abstract

Section: Resultssupporting

confidence: 63%

Section: Resultsmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Hippocampal Long-Term Depression in the Presence of Calcium-Permeable AMPA Receptors

Cao

Zhou

Cai

et al. 2018

Front. Synaptic Neurosci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…At present, the studies of actin are mainly in cytoskeleton structure and rearrangement [ 6 , 7 , 8 , 9 ]. However, little research has been focused on reliable and accurate detection of actin.…”

Section: Introductionmentioning

confidence: 99%

Construction of Chitosan-Zn-Based Electrochemical Biosensing Platform for Rapid and Accurate Assay of Actin

Sun

Zou

Wang

et al. 2018

Sensors

View full text Add to dashboard Cite

This work reports a study on the development of a sensitive immunosensor for the assay of actin, which is fabricated using sensing material chitosan-Zn nanoparticles (NPs) and anti-actin modified on glassy carbon electrode respectively. The prepared materials were characterized using transmission electron microscope (TEM), fourier transform infrared spectra (FTIR), X-ray diffraction (XRD) spectra, and circular dichroism (CD) techniques. Meanwhile, the electrochemical properties were studied by linear sweep voltammetric (LSV), electrochemical impedance spectra (EIS), and differential pulse voltammetry (DPV). According to the experiments, under the optimum conditions, the linear fitting equation was I (μA) = −17.31 + 78.97c (R2 = 0.9948). The linear range was from 0.0001 to 0.1 mg/mL and the detection limit (LOD, S/N = 3) was 21.52 ng/mL. The interference studies were also performed for checking the sensors’ selectivity to actin. With better properties of the chitosan-Zn NPs, the modified electrode is considered as a better candidate than Western blot or immunohistochemical method for real-time usability. The detection limit reported is the lowest till date and this method provides a new approach for quality evaluation.

show abstract

Role of Drebrin in Synaptic Plasticity

Sekino

Koganezawa

Mizui

et al. 2017

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Synaptic plasticity underlies higher brain function such as learning and memory, and the actin cytoskeleton in dendritic spines composing excitatory postsynaptic sites plays a pivotal role in synaptic plasticity. In this chapter, we review the role of drebrin in the regulation of the actin cytoskeleton during synaptic plasticity, under long-term potentiation (LTP) and long-term depression (LTD). Dendritic spines have two F-actin pools, drebrin-decorated stable F-actin (DF-actin) and drebrin-free dynamic F-actin (FF-actin). Resting dendritic spines change their shape, but are fairly constant over time at steady state because of the presence of DF-actin. Accumulation of DF-actin is inversely regulated by the intracellular Ca concentration. However, LTP and LTD stimulation induce Ca influx through N-methyl-D-aspartate (NMDA) receptors into the potentiated spines, resulting in drebrin exodus via myosin II ATPase activation. The potentiated spines change to excited state because of the decrease in DF-actin and thus change their shape robustly. In LTP, the Ca increase via NMDA receptors soon returns to the basal level, and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) expression at the postsynaptic membrane is increased. The Ca recovery and AMPAR increase coordinately induce the re-accumulation of DF-actin and change the dendritic spines from the excited state to steady state during LTP maintenance. During LTD, the prolonged intracellular Ca increase inhibits the re-accumulation of DF-actin, resulting in facilitation of AMPAR endocytosis. Because of the positive feedback loop of the AMPAR decrease and drebrin re-accumulation inhibition, the dendritic spines are instable during LTD maintenance. Taken together, we propose the presence of resilient spines at steady state and plastic spines at excited state and discuss the physiological and pathological relevance of the two-state model to synaptic plasticity.

show abstract

Developmental regulation of hippocampal long-term depression by cofilin-mediated actin reorganization

Cited by 16 publications

References 61 publications

Hippocampal Long-Term Depression in the Presence of Calcium-Permeable AMPA Receptors

Hippocampal Long-Term Depression in the Presence of Calcium-Permeable AMPA Receptors

Construction of Chitosan-Zn-Based Electrochemical Biosensing Platform for Rapid and Accurate Assay of Actin

Role of Drebrin in Synaptic Plasticity

Contact Info

Product

Resources

About