Developmental roles of the glypicans

Cat, Bart De; Gourichon, David

doi:10.1006/scdb.2000.0240

Cited by 147 publications

(102 citation statements)

References 52 publications

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“…Of the seven genes from intraabdominal group, we decided to focus our analysis on the five most significant genes, including two homeo box genes, HoxA5 and HoxC8; Nr2f1, nuclear receptor subfamily 2 group F member 1, also known as COUP-TFI, an orphan member of the steroid receptor superfamily thought to be involved in organogenesis (29); glypican 4 (Gpc4), a cell-surface heparan sulfate proteoglycan involved in cell division and growth regulation (30); and thrombomodulin (Thbd), a surface glycoprotein of endothelial and placental cells (31). We also studied all five genes from the s.c. group of genes including the homeobox gene HoxC9; short stature homeobox 2 (Shox2) a transcription factor with homeodomain expressed during embryonic development (32); Tbox-15 (Tbx15), a transcription factor involved in craniofacial and limb development in the mouse (33); engrailed 1 (En1), the mouse homologue of a Drosophila patterning gene (34); and secreted frizzled-related protein 2 (Sfrp2), a soluble modulator of Wnt signaling (35).…”

Section: Resultsmentioning

confidence: 99%

Evidence for a role of developmental genes in the origin of obesity and body fat distribution

Gesta

Blüher

Yamamoto

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

558

538

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Obesity, especially central obesity, is a hereditable trait associated with a high risk for development of diabetes and metabolic disorders. Combined gene expression analysis of adipocyte-and preadipocyte-containing fractions from intraabdominal and subcutaneous adipose tissue of mice revealed coordinated depotspecific differences in expression of multiple genes involved in embryonic development and pattern specification. These differences were intrinsic and persisted during in vitro culture and differentiation. Similar depot-specific differences in expression of developmental genes were observed in human subcutaneous versus visceral adipose tissue. Furthermore, in humans, several genes exhibited changes in expression that correlated closely with body mass index and͞or waist͞hip ratio. Together, these data suggest that genetically programmed developmental differences in adipocytes and their precursors in different regions of the body play an important role in obesity, body fat distribution, and potential functional differences between internal and subcutaneous adipose tissue.adipose tissue ͉ gene expression ͉ subcutaneous ͉ intraabdominal

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Section: Resultsmentioning

confidence: 99%

Evidence for a role of developmental genes in the origin of obesity and body fat distribution

Gesta

Blüher

Yamamoto

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

558

538

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“…Microarray analysis has also shown the importance of ECM glypicans which are proteins that can bind a multitude of GF and ECM molecules which are implicated in signal transduction cascaded that most likely regulate cell proliferation. Glypicans activate or determine activities of morphogens and GF such as FGF, BMPs and IGFs (Berry et al, 1998;De Cat and David, 2001;Hwang et al, 2001). We have seen that glycipans are up-regulated in fetal dermal skin cells (microarray data, GPC4 and GPC3 with 2.02 and 2.03 up-regulated, respectively) and in another recent study by Lener et al (2006) they had seen the same effect with down-regulation of glycipans in aged skin.…”

Section: Discussionmentioning

confidence: 60%

Chronic wound healing by fetal cell therapy may be explained by differential gene profiling observed in fetal versus old skin cells

Ramelet¹,

Hirt‐Burri

Raffoul³

et al. 2009

Experimental Gerontology

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a b s t r a c tEngineering of fetal tissue has a high potential for the treatment of acute and chronic wounds of the skin in humans as these cells have high expansion capacity under simple culture conditions and one organ donation can produce Master Cell Banks which can fabricate over 900 million biological bandages (9 Â 12 cm). In a Phase 1 clinical safety study, cases are presented for the treatment of therapy resistant leg ulcers. All eight patients, representing 13 ulcers, tolerated multiple treatments with fetal biological bandages showing no negative secondary effects and repair processes similar to that seen in 3rd degree burns. Differential gene profiling using Affymetrix gene chips (analyzing 12,500 genes) were accomplished on these banked fetal dermal skin cells compared to banked dermal skin cells of an aged donor in order to point to potential indicators of wound healing. Families of genes involved in cell adhesion and extracellular matrix, cell cycle, cellular signaling, development and immune response show significant differences in regulation between banked fetal and those from banked old skin cells: with approximately 47.0% of genes over-expressed in fetal fibroblasts. It is perhaps these differences which contribute to efficient tissue repair seen in the clinic with fetal cell therapy.

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“…Gpcs are proteins of around 60-70 kDa with a characteristic pattern of 14-conserved cysteine residues mainly located to the central domain (De Cat and David, 2001). Gpcs also share an N-terminal signal sequence and a hydrophobic C-terminal sequence involved in the formation of the GPI anchor structure (Fig.…”

Section: Glypican Assemblymentioning

confidence: 99%

“…Gpcs also share an N-terminal signal sequence and a hydrophobic C-terminal sequence involved in the formation of the GPI anchor structure (Fig. 1;De Cat and David, 2001). Heparan sulphate glycosaminoglycan (HSGAG) polysaccharide side chains can be attached to serine residues in consensus sequences, such as XGlyXGlySerX, that are located between the central domain and the C-terminal GPI-anchor (De Cat and David, 2001).…”

Section: Glypican Assemblymentioning

confidence: 99%

Signalling Mechanisms Underlying Congenital Malformation: The Gatekeepers, Glypicans

Fico¹,

Dono²

2012

Congenital Anomalies - Case Studies and Mechanisms

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Developmental roles of the glypicans

Cited by 147 publications

References 52 publications

Evidence for a role of developmental genes in the origin of obesity and body fat distribution

Evidence for a role of developmental genes in the origin of obesity and body fat distribution

Chronic wound healing by fetal cell therapy may be explained by differential gene profiling observed in fetal versus old skin cells

Signalling Mechanisms Underlying Congenital Malformation: The Gatekeepers, Glypicans

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