Developmental stimuli and stress factors affect expression of ClGLP1, an emerging allergen-related gene in Citrus limon

Bruno, Leonardo; Spadafora, Natasha D.; Iaria, Domenico; Chiappetta, Adriana; Bitonti, Maria Beatrice

doi:10.1016/j.plaphy.2014.03.003

Cited by 16 publications

(11 citation statements)

References 57 publications

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“…Quantitative real-time PCR (qRT-PCR) was performed as reported in Bruno et al (2014) using a STEP ONE instrument (Applied Biosystems). Primers used for qRT-PCR analysis were designed using Primer3 software, according to Yokoyama and Nishitani (2001).…”

Section: Methodsmentioning

confidence: 99%

In Arabidopsis thaliana Cadmium Impact on the Growth of Primary Root by Altering SCR Expression and Auxin-Cytokinin Cross-Talk

et al. 2017

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Cadmium is one of the most widespread pollutant in both terrestrial and marine environment, and its inhibitory effect on plant growth has been largely demonstrated. However, the molecular mechanisms underlying Cd toxicity in plant and mainly in root, as the first organ sensing soil heavy metals, need to be better investigated. To this aim, in the present work we analyzed the growth and the organization of Arabidopsis thaliana primary root in seedlings exposed to Cd (25 and 50 μM) for 8 days starting from germination. Root length, root meristem size, and organization were evaluated together with the behavior of some of the major molecular players in root growth and patterning. In particular, by using different GFP transgenic lines, we monitored: (i) the expression pattern of WOX5 and SCR transcription factors involved in the establishment and maintenance of stem cell niche and in the control of meristem size; (ii) the expression pattern of the IAA-inducible pDR5::GFP reporter, PIN 1, 2, 3, 7 auxin carriers and TCSn::GFP cytokinin-sensitive sensor as relevant components of hormone circuit controlling root growth. We report that Cd exposure inhibits primary root growth via affecting RAM stem cell niche and root radial pattern. At the molecular level, an impairment of auxin maximum accumulation at the root tip, related to a down-regulation and mislocalisation of PIN proteins, and an enhancement of TCSn::GFP cytokinin-sensitive sensor signal is also detected under Cd treatment, thus suggesting an alteration in the homeostasis of auxin/cytokinin signaling. Moreover, and for the first time Cd toxicity on root growth and pattern has been related to a misexpression of SCR transcription factors which is known to interplay with auxin/cytokinin cross-talk in the control of RAM maintenance and activity.

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Section: Methodsmentioning

confidence: 99%

In Arabidopsis thaliana Cadmium Impact on the Growth of Primary Root by Altering SCR Expression and Auxin-Cytokinin Cross-Talk

et al. 2017

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“…and biological significances like growth determinant, potential allergen activity, etc. Bruno et al 2014;Hu and Sun 2010;Sarkar et al 2010). Expressions and functional significances of several GLPs identified from monocots, dicots, gymnosperms as well as moss have been mentioned below and Supplementary Material (Supplementary Table S2).…”

Section: Expressional Diversity and Functional Significance Of Glp Mementioning

confidence: 99%

“…Those mex1 lines were observed to sustain stress condition raised due to blockage of maltose export, and a GLP (GLP3, At5g20630) was found to be upregulated in those lines (Hwang et al 2013) indicating the involvement of GLPs in those circumstances. A GLP from Citrus limon (ClGLP) was found to show the highest level of expression in mature fruit especially during the transition phase from green to yellow stages of fruit growth (Bruno et al 2014). A change in the redox state of protein thiol group was noticed during leaf senescence and a germin-like protein was identified among that thiol group containing proteins in Arabidopsis (Alvarez et al 2008).…”

Section: Glp Expression At Later Stages Of Growthmentioning

confidence: 99%

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Versatility of germin-like proteins in their sequences, expressions, and functions

Barman

Banerjee

2015

Funct Integr Genomics

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Germin-like proteins (GLPs) are evolutionary conserved ubiquitous plant glycoproteins belonging to the cupin superfamily. A large number of GLP family members have been identified from different higher and lower plant species, and those have been classified into different subfamilies. Although three histidine residues (H) and one glutamate residue (E) in germin box B and C were conserved among all the GLP subfamily members, how the sequences of one subfamily member differ from the other is unclear. Progress in the field of genomics, transcriptomics, and proteomics has made it possible to understand the variation at gene level among different GLP members from diverse genera and also their biological significances. GLPs from different plant species were found to have various enzymatic properties including oxalate oxidase (OxO), superoxide dismutase (SOD), ADP glucose pyrophosphatase/phosphodiesterase (AGPPase), and polyphenol oxidase (PPO) activities. 'Omics' study demonstrated the expression as well as involvement of GLP family members in almost every part of higher plants as well as in lower plants. Additionally, GLPs from different species were reported to be involved in biotic as well as abiotic stresses and also in the growth and development. This review describes the present research status of GLPs from different plant species, their expressions, and functional significances. Sequence variation was detected among GLP subfamily members at the amino acid level, and based on the sequence variation and phylogenetic analyses, two new GLP subfamilies have been proposed in this review.

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“…The fruits of Citrus limon (L.) Burm.f. contain important natural chemical components, such as flavonoids, furanocoumarins, and limonoids [9]. Previous studies have described the biological activities of these compounds, such as the antioxidative, anti-inflammatory, antiallergic, antiviral, antiproliferative, anticarcinogenic, and antimutagenic activities of flavonoids [10], the suppressive effect of limonin on intestinal polyp development in Apc-mutant Min mice [11], and the inhibitory effects of furanocoumarins on human CYP 3A4 [12].…”

Section: Reviewmentioning

confidence: 99%

Isolation and structure elucidation of constituents of Citrus limon, Isodon japonicus, and Lansium domesticum as the cancer prevention agents

Μatsumoto

Watanabe

2020

Genes and Environ

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In the course of our research to investigate the cancer prevention potency of natural products derived from plant materials, we isolated fifty-five compounds, including twenty-one new compounds from the peels of Citrus limon, aerial parts of Isodon japonicus, and leaves of Lansium domesticum. The chemical structures of the isolated compounds were elucidated by chemical/physicochemical evidence, and nuclear magnetic resonance spectroscopy and mass spectrometry results. Moreover, the absolute stereochemistry of the new compounds were elucidated by various techniques such as chemical synthesis, modified Mosher's method, Cu-Kα X-ray crystallographic analysis, and comparison of experimental and predicted electronic circular dichroism data. The antimutagenic effects of the isolated and structure-elucidated compounds against heterocyclic amines, 3-amino-1,4-dimethyl-5H-pyrido [4,3b]indole (Trp-P-1) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), were evaluated by the Ames test and in vivo micronucleus test. In this review, we present the comprehensive results of the antimutagenic effects of the isolated natural products.

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Developmental stimuli and stress factors affect expression of ClGLP1, an emerging allergen-related gene in Citrus limon

Cited by 16 publications

References 57 publications

In Arabidopsis thaliana Cadmium Impact on the Growth of Primary Root by Altering SCR Expression and Auxin-Cytokinin Cross-Talk

In Arabidopsis thaliana Cadmium Impact on the Growth of Primary Root by Altering SCR Expression and Auxin-Cytokinin Cross-Talk

Versatility of germin-like proteins in their sequences, expressions, and functions

Isolation and structure elucidation of constituents of Citrus limon, Isodon japonicus, and Lansium domesticum as the cancer prevention agents

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