Developmental toxicity induced by particulate matter (PM2.5) in zebrafish (Danio rerio) model

Indoor and Personal PM2.5 Samples Differ in Chemical Composition and Alter Zebrafish Behavior Based on Primary Fuel Source

“…22 The morphological effects seen in our study were not surprising, since Manjunatha et al reported similar effects, including cardiac defects, bent tail and spine, underdeveloped vasculature and innervation, liver abnormalities, and swim bladder absence. 22 However, Manjunatha et al used diesel standard reference material, which varies from biomass or LPG combustion, as evidenced by varied PAH concentrations compared to ours. A literature review by Smoot et al highlighted additional studies that reported morphological effects, similar to those found by Manjunatha et al and our present study.…”

“…Manjunatha et al reported significant mortality within 5 days of exposure to as low as 15 μg PM 2.5 /mL and decreased survival within 48 h of exposure to 100 μg PM 2.5 /mL. 22 The morphological effects seen in our study were not surprising, since Manjunatha et al reported similar effects, including cardiac defects, bent tail and spine, underdeveloped vasculature and innervation, liver abnormalities, and swim bladder absence. 22 However, Manjunatha et al used diesel standard reference material, which varies from biomass or LPG combustion, as evidenced by varied PAH concentrations compared to ours.…”

“…A literature review by Smoot et al highlighted additional studies that reported morphological effects, similar to those found by Manjunatha et al and our present study. 22,61 Embryonic Photomotor Response (EPR). The embryonic photomotor response (EPR) is a behavioral response to changing light conditions frequently used as an indicator of biological toxicity, specifically, of later adverse effects on development.…”

The utility of alternative models in particulate matter air pollution toxicology

“…Investigators have also examined PM 2.5 ’s potential for developmental toxicity ( Manjunatha et al, 2021 ). Acute and developmental toxicity assays of zebrafish exposed to PM can be run in a variety of vessels, including glass petri dishes, and welled plates of various sizes (24 to 96-well).…”

Smartphone-based holographic measurement of polydisperse suspended particulate matter with various mass concentration ratios

“…Airborne PMs consisting of heterogeneous chemical compounds 4 can easily penetrate into the human body, causing severe health problems [5][6][7] . In water, suspended PMs can bring about adverse effects on aquatic ecosystems 8 , such as developmental toxicity 9 and light attenuation that hinders photosynthesis and nutrient uptake of aquatic organisms 10 . Therefore, precise monitoring of suspended PMs is needed to prevent its adverse effects on public health and nature.…”