Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae: Disrupted vascular formation with angiogenic failure and inhibited neurogenesis

Park, Hahyun; Lee, Jin Young; Park, Sung Soo; Song, Gwonhwa; Lim, Whasun

doi:10.1016/j.jhazmat.2019.121531

Cited by 60 publications

(26 citation statements)

References 54 publications

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“…AChE inhibition by neurotoxic chemicals is a well-known response; in this case a significant increase in all brain B-esterases (at both temperatures) could not be linked to temperature increases as formerly reported in brain AChE of D. labrax by . In the present study, although caspases were not measured, B-esterase activity increases may be related to apoptosis as revealed in zebrafish larvae exposed to fipronil (Park, 2020). Recent evidence of the link between fipronil effects and CE activity patterns comes from a study by Jung et al (2020) that revealed that the administration of a CE inhibitor reversed apoptosis in mouse brain.…”

Section: B-esterase Modulation By Temperature and Fipronil Exposurementioning

confidence: 53%

“…In rats, the parent form and the sulfone metabolite can cross the blood-brain barrier and accumulate in the brain (Cravedi et al, 2013). Moreover, exposure to fipronil triggered apoptosis in a human neural cell line (Vidau et al, 2011) and, as previously mentioned, caused overall apoptosis in early stages of development of D. rerio (Park, 2020).…”

Section: B-esterase Modulation By Temperature and Fipronil Exposurementioning

confidence: 67%

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Multi-organ characterisation of B-esterases in the European sea bass (Dicentrarchus labrax): Effects of the insecticide fipronil at two temperatures

et al. 2020

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Section: B-esterase Modulation By Temperature and Fipronil Exposurementioning

confidence: 53%

Section: B-esterase Modulation By Temperature and Fipronil Exposurementioning

confidence: 67%

Multi-organ characterisation of B-esterases in the European sea bass (Dicentrarchus labrax): Effects of the insecticide fipronil at two temperatures

et al. 2020

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“…In the zebrafish embryo, development of motor circuits is disturbed by fipronil at 0.7 µM, most likely by interaction of fipronil with glycin receptors (Stehr et al 2006). At higher concentrations (~ 33 µM), fipronil inhibits zebrafish neurogenesis via reduction of gene expression important for neuron/glia differentiation (Park et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…In our daily life, a wide range of activities in agriculture, industry, and even the management of the own households is associated with using a diversity of chemicals, including pesticides (Schäfer et al 2011). Insecticides do not only accumulate within the environmental circulation (Park et al 2020); they are also absorbed via dietary intake or indoor and outdoor dust (Kim et al 2019;Mahler et al 2009). In addition to acute toxicity of high dose exposure, there are concerns about low-dose, long-term effects on human health.…”

Section: Introductionmentioning

confidence: 99%

“…The DNT status of fipronil is not yet finally agreed on. Several in vivo studies describe neurotoxic effects of fipronil in zebrafish development (Stehr et al 2006;Park et al 2020), rats (Cravedi et al 2013;Abdel-Daim et al 2019) and mice (Badgujar et al 2016). In vivo testing of chemicals in vertebrates is cost-intensive and raises severe ethical concerns (Russell and Burch, 1959).…”

Section: Introductionmentioning

confidence: 99%

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Developmental Neurotoxicity of Fipronil and Rotenone on a Human Neuronal In Vitro Test System

Schmitz¹,

Dempewolf²,

Tan³

et al. 2021

Neurotox Res

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Pesticide exposure during in utero and early postnatal development can cause a wide range of neurological defects. However, relatively few insecticides have been recognized as developmental neurotoxicants, so far. Recently, discovery of the insecticide, fipronil, in chicken eggs has raised public concern. The status of fipronil as a potential developmental neurotoxicant is still under debate. Whereas several in vivo and in vitro studies suggest specific toxicity, other in vitro studies could not confirm this concern. Here, we tested fipronil and its main metabolic product, fipronil sulfone both at concentrations between 1.98 and 62.5 µM, alongside with the established developmental neurotoxicant, rotenone (0.004–10 µM) in vitro on the human neuronal precursor cell line NT2. We found that rotenone impaired all three tested DNT endpoints, neurite outgrowth, neuronal differentiation, and precursor cell migration in a dose-dependent manner and clearly separable from general cytotoxicity in the nanomolar range. Fipronil and fipronil sulfone specifically inhibited cell migration and neuronal differentiation, but not neurite outgrowth in the micromolar range. The rho-kinase inhibitor Y-27632 counteracted inhibition of migration for all three compounds (EC50 between 12 and 50 µM). The antioxidant, n-acetyl cysteine, could ameliorate the inhibitory effects of fipronil on all three tested endpoints (EC 50 between 84 and 164 µM), indicating the involvement of oxidative stress. Fipronil sulfone had a stronger effect than fipronil, confirming the importance to test metabolic products alongside original pesticides. We conclude that in vitro fipronil and fipronil sulfone display specific developmental neurotoxicity on developing human model neurons.

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Magnetic Solid‐Phase Extraction of Fipronil from Aqueous Solution and Eggshells Using Amine‐Coated Iron Oxide Nanoparticles

Aran

Bayraç

2023

ChemPlusChem

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Fipronil, as a broad use insecticide, is regulated under directives and standards in many countries since it is classed as Class II moderately hazardous pesticide and Group C possible human carcinogen. In this study, amine coated iron oxide (NH 2 À Fe 3 O 4 ) is used as a new adsorbent with high adsorption capacity in the removal of fipronil from aqueous solution and eggshells.The adsorption performance was studied through batch adsorption under different concentrations of fipronil (10-200 μg/mL), different nanoparticles amount (0.1-1 mg) at different pH (4.5-6.0) and temperature (25-75 °C). Results showed that NH 2 À Fe 3 O 4 nanoparticles (0.1 mg) have excellent adsorption efficiency (97.06 %) at 25 °C, and pH 5.5. It also showed higher adsorption capacity towards fipronil sulfide, fipronil sulfone and fipronil desulfinyl with removal efficiencies of 92.82 %, 86.35 % and 76.24 % from aqueous solution and 97.62 %, 76.97 % and 62.65 % from eggshells, respectively. Fipronil adsorption process on NH 2 À Fe 3 O 4 nanoparticles showed best fitting to Langmuir adsorption isotherm indicating monolayer chemical adsorption by physicochemical interactions spontaneously on homogenous surfaces. Having higher adsorption capacity and reusability NH 2 À Fe 3 O 4 nanoparticles were shown to be effective adsorbents for removal of fipronil from aqueous solution and eggshells.

show abstract

Developmental toxicity of fipronil in early development of zebrafish (Danio rerio) larvae: Disrupted vascular formation with angiogenic failure and inhibited neurogenesis

Cited by 60 publications

References 54 publications

Multi-organ characterisation of B-esterases in the European sea bass (Dicentrarchus labrax): Effects of the insecticide fipronil at two temperatures

Multi-organ characterisation of B-esterases in the European sea bass (Dicentrarchus labrax): Effects of the insecticide fipronil at two temperatures

Developmental Neurotoxicity of Fipronil and Rotenone on a Human Neuronal In Vitro Test System

Magnetic Solid‐Phase Extraction of Fipronil from Aqueous Solution and Eggshells Using Amine‐Coated Iron Oxide Nanoparticles

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