Developments and opportunities in fungal strain engineering for the production of novel enzymes and enzyme cocktails for plant biomass degradation

Kun, Roland S.; Gomes, Ana Carolina S.; Hildén, Kristiina; Salazar-Cerezo, Sonia; Mäkelä, Miia; Vries, Ronald P. de

doi:10.1016/j.biotechadv.2019.02.017

Cited by 52 publications

(28 citation statements)

References 231 publications

(219 reference statements)

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“…These facts illustrate the possibility to develop low-cost degradation enzyme cocktails with high efficiency [2,26]. The high levels of lignocellulolytic enzymes and rapid enzyme production capacity of T. harzianum EM0925 made it a suitable candidate for enzyme cocktail production, since most of the previous studies on developing cost-effective cell wall degrading enzymes have focused on improving the activity of cellulase by adopting genetic operation and screening natural strains so as to obtain high yield of glucose [4,35]. Meng et al [36] overexpressed the endoglucanase gene in T. reesei Rut-C30, which showed 90.0% and 132.7% increases in the activities of total cellulases and endoglucanases under flask culture conditions, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Zhang

Yang

Luo

et al. 2020

IJMS

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Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the commercial enzyme preparations, the T. harzianum EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting T. harzianum EM0925 enzymes have great potential for industrial applications.

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Section: Discussionmentioning

confidence: 99%

“…These enzymes can be produced by many fungi (like Aspergillus and Trichoderma) and bacteria (like Cellulomonas and Clostridium) [2,3]. In any case, the rapid and complete conversion of cellulose is the limiting step during the process of bio-refinery [4].…”

Section: Introductionmentioning

confidence: 99%

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Zhang

Yang

Luo

et al. 2020

IJMS

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“…In his article, Kun et al (2019) examined the degradation of plant biomass in a special way, for example, CRISPR/Cas9 genome editing and adaptive evolution.…”

Section: Modify the Enzymes And Increase Their Efficiencymentioning

confidence: 99%

An overview of biomass conversion: exploring new opportunities

Fülöp¹,

Ecker²

2020

PeerJ

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Recycling biomass is indispensable these days not only because fossil energy sources are gradually depleted, but also because pollution of the environment, caused by the increasing use of energy, must be reduced. This article intends to overview the results of plant biomass processing methods that are currently in use. Our aim was also to review published methods that are not currently in use. It is intended to explore the possibilities of new methods and enzymes to be used in biomass recycling. The results of this overview are perplexing in almost every area. Advances have been made in the pre-treatment of biomass and in the diversity and applications of the enzymes utilized. Based on molecular modeling, very little progress has been made in the modification of existing enzymes for altered function and adaptation for the environmental conditions during the processing of biomass. There are hardly any publications in which molecular modeling techniques are used to improve enzyme function and to adapt enzymes to various environmental conditions. Our view is that using modern computational, biochemical, and biotechnological methods would enable the purposeful design of enzymes that are more efficient and suitable for biomass processing.

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“…Advances in the production of recombinant enzymes focus on the search for industrially viable microorganisms capable of producing enzymes under various conditions, expressing them in a highly efficient manner, aiming at the synthesis of several copies of genes and a strong promoter. Several species of fungi are capable of synthesizing and secreting high amounts of cellulase; most studies with fungal species use linearized plasmid, since these are encompassed to chromosomal DNA, improving its stability and expression efficiency [11].…”

Section: Introductionmentioning

confidence: 99%

Recombinant Fungal Cellulases for the Saccharification of Sugarcane Bagasse

Benevides¹,

Assis²,

Vitor³

et al. 2022

Biodegradation Technology of Organic and Inorganic Pollutants

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Cellulases are important enzymes in cellulose degradation that occurs in nature, this degradation involves a system of extracellular multienzymes and have wide application. The construction of a high-quality system for the production of these enzymes is important for its application in the process of saccharification of biomass involved in the biofuel production process. Several species of fungi are capable of synthesizing and secreting high amounts of cellulase, most studies with fungal species use linearized plasmid, since these are encompassed to chromosomal DNA, improving its stability and expression efficiency. Advances in the production of recombinant enzymes focus on the search for industrially viable microorganisms capable of producing enzymes under various conditions, expressing them in a highly efficient manner, aiming at the synthesis of several copies of genes and a strong promoter. To resay these restrictions, molecular biology combined with recombinant DNA technology is a viable tool in enzymatic production. In subsequent topics, the production of endoglucanases, exoglucanases and β-glucosidase of fungi cloned in Escherichia coli, Pichia pastoris and other different expression systems will be addressed.

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Developments and opportunities in fungal strain engineering for the production of novel enzymes and enzyme cocktails for plant biomass degradation

Abstract: , et al., Developments and opportunities in fungal strain engineering for the production of novel enzymes and enzyme cocktails for plant biomass degradation, Biotechnology Advances,

Cited by 52 publications

References 231 publications

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

An overview of biomass conversion: exploring new opportunities

Recombinant Fungal Cellulases for the Saccharification of Sugarcane Bagasse

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