Devosia lucknowensis sp. nov., a bacterium isolated from hexachlorocyclohexane (HCH) contaminated pond soil

Abstract

Strain L15(T), a Gram-negative, motile, orange colored bacterium was isolated from pond soil in the surrounding area of a hexachlorocyclohexane (HCH) dump site at Ummari village in Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain L15(T) belongs to the family Hyphomicrobiaceae in the order Rhizobiales. Strain L15(T) showed highest 16S rRNA gene sequence similarity to Devosia chinhatensis IPL18(T) (98.0%). Chemotaxonomic data revealed that the major fatty acids were summed feature 8 (C18:1 ω7c and/or C18:1 ω6c), C18:1 ω7c 11-methyl, C16:0 and C18:0. The major polar lipids of strain L15(T) were diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G+C content of strain L15(T) was 59.8%. Polyamine profile showed the presence of sym-homospermidine with traces of putrescine. Ubiquinone Q-10 was the major respiratory quinone present. Based on these data, strain L15(T) (=CCM 7977(T) =DSM 25398(T)) was classified as a type strain of a novel species, for which the name Devosia lucknowensis sp. nov. is proposed.

Devosia marina sp. nov., isolated from deep seawater of the South China Sea, and reclassification of Devosia subaequoris as a later heterotypic synonym of Devosia soli

“…The polar lipid profile of strain L53-10-65 T was similar to that of the type strain of 'D. lukinowensis' [11], in that DPG, PG and GL were major polar lipids [8,11]. The respiratory quinone of strain L53-10-65 T was identified as Q-10 (100 %), in line with all members of the genus Devosia.…”

“…The isolated strain was maintained at −80 °C supplemented with a 20 % (v/v) glycerol suspension for extended preservation. For comparison with the isolated strain, reference strains Devosia indica IO390501 T [8] (a private gift), 'Devosia lucknowensis' L15 T (=DSM 25398 T ) [11] from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH and Devosia riboflavina IFO 13584 T (=JCM 21244 T , the type species of the genus Devosia) [1] from the Japan Collection of Microorganisms were obtained.…”

Defining the Environmental Adaptations of Genus Devosia: Insights into its Expansive Short Peptide Transport System and Positively Selected Genes

“…Genomic DNA extraction and sequencing. D. crocina IPL20 and D. lucknowensis L15 were isolated from soils contaminated with hexachlorocyclohexane (HCH) from dumpsites located at Chinhat and Ummari villages in Lucknow, India 8,19 . The strains were grown on Luria-Bertani (LB) agar incubated at 28 °C and genomic DNA was isolated by lysis with lysozyme and proteinase K followed by CTAB extraction using method described elsewhere 75 .…”

“…In an effort to characterize the culturable diversity of soil contaminated with HCH [12][13][14][15][16][17][18] , we isolated and characterized four novel members of the genus Devosia viz. D. chinhatensis IPL18 7 , D. crocina IPL20 8 , D. albogilva IPL15 8 and D. lucknowensis L15 19 . Although isolated from HCH contaminated soil, these isolates were not able to degrade HCH isomers 8 .…”

Survey of (Meta)genomic Approaches for Understanding Microbial Community Dynamics

“…With the availability of sequenced bacterial genomes, comparative genomics has emerged to be indispensable in elucidating evolutionary forces active across genera or species; however it remains incompetent to demonstrate results at the population level of in situ cohorts in an environment [1][2][3][4][5][6][7][8][9][10][11]. The problem can be resolved to some extent by using genomics and metagenomics data together delineating the pan-genome dynamics of a community distinctly elucidating environment specific lifestyles adopted by bacteria ( Fig.…”