Dexamethasone-containing bioactive dressing for possible application in post-operative keloid therapy

Abstract

Bioactive dressing based on bacterial cellulose modified with carboxymethyl groups (mBC) was successfully prepared and studied. The surface of mBC was activated using carbodiimide chemistry and decorated with alginate/hydroxypropyl cellulose submicroparticles containing dexamethasone phosphate (DEX-P). Prior to their deposition particles were coated with chitosan in order to facilitate their binding to mBC, and to increase the control over the release process. The detailed physicochemical characterization of the particles and the bioactive dressing was performed, including the determination of the particles' size and size distribution, DEX-P encapsulation efficiency and loading, particles' distribution on the surface of the mBC membrane, as well as DEX-P release profiles from free and mBC-bound particles. Finally, the preliminary cytotoxicity studies were performed. The fabricated bioactive material releases DEX-P in a controlled manner for as long as 25 h. Biological tests in vitro indicated that the dexamethasone-containing submicroparticles are not toxic toward fibroblasts, while effectively inhibiting their proliferation. The prepared bioactive dressing may be applied in the treatment of the post-operative wounds in the therapy of keloids and in other fibrosis-related therapies.

Immune cells and associated molecular markers in dermal fibrosis with focus on raised cutaneous scars

“…Current treatment approaches include topical anti-scarring application, surgical excision, cryotherapy, pressure dressing, laser therapy, intralesional corticosteroid injections, and antimetabolites. [32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48] The aim of this review is to briefly describe cutaneous wound healing processes and the participating immune cells, then provide a comprehensive overview of the immune and associated molecular targets involved in raised dermal scarring conditions.…”

Pioglitazone-Loaded Nanostructured Hybrid Material for Skin Ulcer Treatment

“…ALG and HPC were used as the main components of the nanoparticles' matrix. We have used the same components previously to encapsulate other biologically active agents [21][22][23] and we have found that the ratio of both polymers must be adjusted individually to each active compound being delivered. In the case of PIO, due to its moderate solubility in water, a surfactant (Pluronic ® P103 or Tween ® 85) micelles were used to facilitate the encapsulation of PIO in the relatively hydrophilic ALG/HPC matrix.…”

“…To deposit chitosan-coated nanospheres on the surface of bacterial nanocellulose modified with carboxyl groups (mBNC), a procedure described and optimized in our previous work was applied [21]. A 4 cm 2 patch of mBNC was immersed in an acetate buffer (pH = 5.5) at room temperature.…”

Recent Reports on Polysaccharide-Based Materials for Drug Delivery

“…Another inexpensive and biocompatible CL derivative is hydroxypropyl cellulose. It was used as a component of bioactive dressing with corticosteroid dexamethasone as an active agent [ 151 ]. ALG and hydroxypropyl CL were applied for the drug encapsulation and crosslinked with calcium ions.…”