Dexamethasone inhibits α-fetoprotein gene transcription in neonatal rat liver and isolated nuclei

Huang, Dao-Pei; Cote, Gilbert J.; Massari, Ronald J.; Chiu, Jen-Fu

doi:10.1093/nar/13.11.3873

Cited by 28 publications

(5 citation statements)

References 52 publications

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“…There is thus a possible link between the suggested role of glucocorticoids and changes in p21 CIP1 expression. However, the synthetic glucocorticoid dexamethasone administered to newborn rats also reduces AFP mRNA and the production of the protein (Huang et al 1985). In our studies, we have not found significant changes in AFP in either liver or kidney, suggesting either that the AFP gene is less sensitive to glucocorticoids than the p21 CIP1 gene or that the changes in the kidney are mediated through another mechanism.…”

Section: Discussioncontrasting

confidence: 61%

The expression of growth-arrest genes in the liver and kidney of the protein-restricted rat fetus

Maloney¹,

Lilley²,

Cruickshank³

et al. 2005

Br J Nutr

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During fetal life, there are periods of rapid cell proliferation, which are uniquely sensitive to nutritional perturbation. Feeding the pregnant rat a proteinrestricted diet alters the growth trajectory of major fetal organs such as the kidney. By day 21 of gestation, the ratio of kidney weight to total body weight is reduced in the fetuses of dams fed a protein-deficient diet. In contrast, the ratio of fetal liver weight to total body weight is unchanged. To investigate the mechanisms underlying this disproportionate change in organ growth in the low-protein group, cell proliferation and differentiation have been assessed in the liver and kidney. The steady-state levels of mRNA for the growth-arrest and DNA-damage gene gadd153/CHOP-10, CCAAT enhancer-binding proteins a and b were unaffected by maternal diet in both fetal liver and kidney. The mRNA for alpha-fetoprotein, albumin and hepatic glucokinase were unchanged in the liver, suggesting that maternal protein deficiency does not alter the state of differentiation. The steady-state levels of the mRNA coding for the cyclindependent protein kinase inhibitors (p15 INK4a, p19INK4d , p21 CIP1

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Section: Discussioncontrasting

confidence: 61%

The expression of growth-arrest genes in the liver and kidney of the protein-restricted rat fetus

Maloney¹,

Lilley²,

Cruickshank³

et al. 2005

Br J Nutr

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“…In rat hepatoma cell lines, effects of DEX on AFP expression are either stimulatory (Tsukada er al., 1985) or suppressive (Huang et al, 1985) depending on the cells used. The cause of such variations is not known at present.…”

Section: Discussionmentioning

confidence: 99%

Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC‐M and HCC‐T

Saito

Morizane

Watanabe

et al. 1991

Intl Journal of Cancer

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The in vitro effect of sodium butyrate (SB) on human hepatoma cell lines PLC/PRF/5, HCC-M and HCC-T was investigated. SB was added at the non-toxic but cytostatic concentration of 1 mM. In all these cell lines, SB reduced cell proliferation and changed the morphology of the cells into a fibroblast-like shape. In PLC/PRF/5, alpha-fetoprotein production and c-myc expression were inhibited. In contrast, gene expression of albumin, one of the normal liver-cell products, and that of integrated hepatitis B virus genome, was increased. In HCC-M and HCC-T, c-myc expression, which was enhanced in the naive state, was reduced. In HCC-M, fos expression was inhibited but the expression of N- and K-ras genes did not change. SB seemed to induce normal or mature properties of hepatocytes in human hepatoma cell lines.

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“…It has been demonstrated that dexamethasone inhibits aFP synthesis by decreasing the specific mRNA content of newborn rat liver in vivo (Chiu et al 1981, Huang et al 1985 and also in fetal rat (Chou 1988) (1989) also showed that a marked inhibition (up to eightfold) of aFP secretion was produced by 10~8 m T3 in Hep G2 cells. However, they reported that the secretion of albumin was stimulated (up to fourfold).…”

Section: Discussionmentioning

confidence: 97%

Actions of 3,5,3′-tri-iodothyronine on the synthesis and secretion of major plasma proteins by a human hepatoblastoma cell line (Hep G2)

Kobayashi

Horiuchi²

1995

Journal of Molecular Endocrinology

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We have elucidated the action of tri-iodothyronine (T3) on the synthesis and secretion of seven major plasma proteins in a human hepatoblastoma cell line, Hep G2, and established an in vitro experimental model of human liver cells for the study of the mechanism of the action of thyroid hormone. Hep G2 cells cultured in serum-free medium were treated with various concentrations of T3. During the first 24 h of T3 treatment, accumulation of alpha-fetoprotein in the medium was decreased in a dose-dependent manner (10(-11)-10(-8) M), and the inhibitory effect was enhanced during the second 24 h of T3 treatment. On the other hand, alpha 1-antitrypsin accumulation in the medium during the second 24 h of hormone treatment was decreased by T3 (10(-9)-10(-8) M), although no change in accumulation was observed during the first 24 h of T3 treatment. The newly synthesized [35S]Met-labelled alpha 1-acid glycoprotein was increased by T3 and reached 3.4-fold within 37 h of 10(-8) M T3 treatment. The stimulatory effect increased time-dependently (4.6-fold after 61 h). In contrast, the synthesis of alpha-fetoprotein was reduced to half of that of the control after T3 treatment for 37 h. Although the content of newly synthesized [35S]alpha 1-antitrypsin was not affected by 10(-8) M T3 treatment during 3 days of hormone treatment, the accumulation of alpha 1-antitrypsin in the medium decreased to 87%; in contrast, total cellular newly synthesized alpha 1-antitrypsin increased to 105-130% of that of the control.(ABSTRACT TRUNCATED AT 250 WORDS)

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Dexamethasone inhibits α-fetoprotein gene transcription in neonatal rat liver and isolated nuclei

Cited by 28 publications

References 52 publications

The expression of growth-arrest genes in the liver and kidney of the protein-restricted rat fetus

The expression of growth-arrest genes in the liver and kidney of the protein-restricted rat fetus

Differentiating effect of sodium butyrate on human hepatoma cell lines PLC/PRF/5, HCC‐M and HCC‐T

Actions of 3,5,3′-tri-iodothyronine on the synthesis and secretion of major plasma proteins by a human hepatoblastoma cell line (Hep G2)

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