Dexamethasone quantification in dried blood spot samples using LC–MS: The potential for application to neonatal pharmacokinetic studies

Patel, Parul; Tanna, Sangeeta; Mulla, Hussain; Kairamkonda, Venkatesh; Pandya, Hitesh; Lawson, Graham

doi:10.1016/j.jchromb.2010.10.009

Cited by 52 publications

(46 citation statements)

References 32 publications

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“…The mobile phase was composed of 0.13% aqueous formic acid:ACN (70:30), and the flow rate was 0.2 mL/min. The method was developed based on defined conditions described in literature, with some modifications [31]. The injection volume was 25 μL, and the column oven temperature was programmed at 35°C.…”

Section: Lc-ms Analysismentioning

confidence: 99%

Transcorneal iontophoresis of dendrimers: PAMAM corneal penetration and dexamethasone delivery

Souza

Dias

Silva

et al. 2015

Journal of Controlled Release

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Section: Lc-ms Analysismentioning

confidence: 99%

Transcorneal iontophoresis of dendrimers: PAMAM corneal penetration and dexamethasone delivery

Souza

Dias

Silva

et al. 2015

Journal of Controlled Release

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“…Advances in instrumental techniques [2] have enabled the analysis of DBS samples to be expanded to include therapeutic drug monitoring [3,4], paediatric pharmacokinetic (PK) studies [5][6][7][8], and metabolite identification investigations [9,10]. In this investigation a DBS analytical approach is developed for the routine monitoring of caffeine, used to treat Apnea of Prematurity (AoP) in neonates, that is, children less than 1 month old.…”

Section: Introductionmentioning

confidence: 99%

Dried Blood Spot Sampling with LC-MS Analysis for Routine Therapeutic Caffeine Monitoring in Neonates

Lawson

Patel

Mulla

et al. 2012

ISRN Chromatography

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A liquid chromatography-mass spectrometry (LC-MS) method was developed and validated for the determination of therapeutic levels of caffeine in dried blood spot (DBS) samples. Caffeine is used in the treatment of Apnoea of Prematurity (AoP) in newborn children. Calibration DBS samples were prepared by spotting 15 µL of whole blood spiked with the analyte onto specimen collection cards. 3 mm disks cut from the centre of the DBS were extracted in methanol containing the internal standard. The extract was separated using a Zorbax Eclipse Plus C18 column and the MS, operated in electrospray positive ion mode, used single ion monitoring at m/z 195 for caffeine and m/z 198 for the IS. The overall extraction recovery of caffeine from spiked blood spots was demonstrated to be 44-47%. Validation of the microanalytical method showed good precision (coefficient of variation) and accuracy (relative error) and specificity and was linear within the tested calibration range 500-25000 ng/mL for caffeine. Investigation of different specimen collection papers revealed different matrix effects with significant ion suppression from the FTA Elute paper itself. Requiring only a microvolume (15 µL) blood sample for analysis, the developed DBS based microanalytical method has the potential to facilitate the routine monitoring of caffeine in neonates.

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“…Micro-samples have reduced the volumes of blood required for analysis, and several studies have demonstrated their usefulness in estimating PK parameters in neonates and children. [7][8][9] Similarly, the technique of population-based parametric PK estimation has been used to facilitate fewer samples for each individual child.…”

Section: Introductionmentioning

confidence: 99%

Invasiveness of Pharmacokinetic Studies in Children–a Systematic Review

2015

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Dexamethasone quantification in dried blood spot samples using LC–MS: The potential for application to neonatal pharmacokinetic studies

Cited by 52 publications

References 32 publications

Transcorneal iontophoresis of dendrimers: PAMAM corneal penetration and dexamethasone delivery

Transcorneal iontophoresis of dendrimers: PAMAM corneal penetration and dexamethasone delivery

Dried Blood Spot Sampling with LC-MS Analysis for Routine Therapeutic Caffeine Monitoring in Neonates

Invasiveness of Pharmacokinetic Studies in Children–a Systematic Review

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