Dextran‐magnetite particles: Contrast‐enhanced MRI of blood–brain barrier disruption in a rat model

Bulte, JW; Jonge, Mwa de; Kamman, R. L.; Go, K.G.; Zuiderveen, F.; Blaauw, Bert; Oosterbaan, J. A.; Leij, Lou de

doi:10.1002/mrm.1910230203

Cited by 41 publications

(20 citation statements)

References 15 publications

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“…In this study ultra-small particles of iron oxide (USPIO) were chosen because they have a very strong reducing effect on the signal intensity in T 2 -weighted imaging, which might help to find minor permeability changes in the pre-paretic period. An additional feature of USPIO is that they can be internalized by cells with macrophagic activity [22][23][24], such as macrophages, microglial cells, and also tumor cells and endothelial cells [25,26]. This proved to be a valuable tool to detect inflammatory areas [23,24].…”

Section: Introductionmentioning

confidence: 99%

Characterization of late radiation effects in the rat thoracolumbar spinal cord by MR imaging using USPIO

Philippens¹,

Gambarota²,

Pikkemaat³

et al. 2004

MAGMA

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The aim of this study was to detect late radiation effects in the rat spinal cord using MR imaging with ultra-small particles of iron oxide (USPIO) contrast agent to better understand the development of late radiation damage with emphasis on the period preceding neurological signs. Additionally, the role of an inflammatory reaction was assessed by measuring macrophages that internalized USPIO. T2-weighted spin echo MR measurements were performed at 7T in six rats before paresis was expected (130-150 days post-irradiation, early group), and in six paretic rats (150-190 days post-irradiation, late group). Measurements were performed before, directly after and, only in the early group, 40 h after USPIO administration and compared with histology. In the early group, MR images showed focal regions in grey matter (GM) and white matter (WM) with signal intensity reduction after USPIO injection. Larger lesions with contrast enhancement were located in and around edematous GM of three animals of the early group and five of the late group. Forty hours after injection, additional lesions in WM, GM and nerve roots appeared in animals with GM edema. In the late paretic group, MR imaging showed WM necrosis adjacent to areas with large contrast enhancement. In conclusion, detection of early focal lesions was improved by contrast administration. In the animals with extended radiation damage, large hypo-intense regions appeared due to USPIO, which might be attributed to blood spinal cord barrier breakdown, but the involvement of blood-derived iron-loaded macrophages could not be excluded.

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Section: Introductionmentioning

confidence: 99%

Characterization of late radiation effects in the rat thoracolumbar spinal cord by MR imaging using USPIO

Philippens¹,

Gambarota²,

Pikkemaat³

et al. 2004

MAGMA

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“…Immediately the suspension was brought to 4OC and SV-magnetoliposomes were separated from uncomplexed SV andlor unencapsulated DMP by washing four times by centrifugation at 15000 x g for 5 min. The final pellet containing the magnetoliposomes was resuspended in BSS at 5 mg lipid/ml, stored at 4OC in dialysis tubing, and used for the labeling of cells within 48 h. The DMP-concentration of magnetoliposomes was assessed by graphite-furnace atomic absorption spectrophotometry as described for the assessment of DMP in plasma samples (27).…”

Section: Introductionmentioning

confidence: 99%

Selective MR imaging of labeled human peripheral blood mononuclear cells by liposome mediated incorporation of dextran‐magnetite particles

Bulte

Loralie

Magin

et al. 1993

Magnetic Resonance in Med

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129

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Human peripheral blood mononuclear cells (PBMCs) were incubated with large unilamellar vesicles (LUV) containing encapsulated dextran-magnetite particles (DMP). This resulted in an efficient incorporation of DMP. Electron microscopy revealed the presence of DMP in cells mainly in phagosomes and secondary lysosomes. DMP-labeled PBMCs showed a strong increase of the transverse relaxation rate (up to 16.6 s-1 for 5 x 10(7) cells/ml) and, accordingly, a great loss of signal intensity in MR imaging. The fraction of DMP containing PBMCs could be enriched by magnetic cell separation. The major population of the DMP containing cells proved to be monocytes. When PBMCs depleted of monocytes were used for labeling, DMP uptake was observed also in the peripheral blood lymphocytes. The labeling of PBMCs presented here may be used in future studies of selective MR imaging of in vivo cell migration in a variety of immunologically compromised tissue states, e.g., tumors, transplantations, and abscesses.

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“…Brain edema induced by cold injury (cold lesion, cryogenic cortical lesion, freeze-traumatized lesion) is a well-known experimental model simulating trau matic brain edema, i.e., vasogenic brain edema, 1,5,14) and has been studied extensively. The disruption of the blood-brain barrier (BBB) associated with vaso genic edema has been extensively investigated since BBB disruption is considered to be important in the development of vasogenic brain edema.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Resonance Imaging of Cold Injury-induced Brain Edema in Rats

Houkin

Abe

Hashiguchi

et al. 1996

Neurol. Med. Chir.(Tokyo)

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The chronological changes of blood-brain barrier disruption, and diffusion and absorption of edema fluid were investigated in rats with cold-induced brain injury (vasogenic edema) using magnetic resonance imaging. Contrast medium was administered intravenously at 3 and 24 hours after lesioning as a tracer of edema fluid. Serial T1-weighted multiple-slice images were obtained for 180 minutes after contrast administration.Disruption of the blood-brain barrier was more prominent at 24 hours after lesioning than at 3 hours. Contrast medium leaked from the periphery of the injury and gradually diffused to the center of the lesion. Contrast medium diffused into the corpus callosum and the ven tricular system (cerebrospinal fluid). Disruption of the blood-brain barrier induced by cold injury was most prominent at the periphery of the vasogenic edema. Edema fluid subsequently extended into the center of the lesion and was also absorbed by the ventricular system. Magnetic resonance imaging is a useful method to assess the efficacy of therapy for vasogenic edema.

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Dextran‐magnetite particles: Contrast‐enhanced MRI of blood–brain barrier disruption in a rat model

Cited by 41 publications

References 15 publications

Characterization of late radiation effects in the rat thoracolumbar spinal cord by MR imaging using USPIO

Characterization of late radiation effects in the rat thoracolumbar spinal cord by MR imaging using USPIO

Selective MR imaging of labeled human peripheral blood mononuclear cells by liposome mediated incorporation of dextran‐magnetite particles

Magnetic Resonance Imaging of Cold Injury-induced Brain Edema in Rats

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