DGAT1-exon8 polymorphism in Anatolian buffalo

et al. 2020

BMC Genet

Background: The diacylglycerol acyltransferases (DGAT) are a vital group of enzymes in catalyzing triacylglycerol biosynthesis. DGAT genes like DGAT1 and DGAT2, have been identified as two functional candidate genes affecting milk production traits, especially for fat content in milk. Buffalo milk is famous for its excellent quality, which is rich in fat and protein content. Therefore, this study aimed to characterize DGAT family genes in buffalo and to find candidate markers or DGAT genes influencing lactation performance. Results: We performed a genome-wide study and identified eight DGAT genes in buffalo. All the DGAT genes classified into two distinct clades (DGAT1 and DGAT2 subfamily) based on their phylogenetic relationships and structural features. Chromosome localization displayed eight buffalo DGAT genes distributed on five chromosomes. Collinearity analysis revealed that the DGAT family genes were extensive homologous between buffalo and cattle. Afterward, we discovered genetic variants loci within the genomic regions that DGAT genes located in buffalo. Seven haplotype blocks were constructed and were associated with buffalo milk production traits. Single marker association analyses revealed four most significant single nucleotide polymorphisms (SNPs) mainly affecting milk protein percentage or milk fat yield in buffalo. Genes functional analysis indicated that these DGAT family genes could influence lactation performance in the mammal through regulating lipid metabolism.Conclusion: In the present study, we performed a comprehensive analysis for the DGAT family genes in buffalo, which including identification, structural characterization, phylogenetic classification, chromosomal distribution, collinearity analysis, association analysis, and functional analysis. These findings provide useful information for an in-depth study to determine the role of DGAT family gens play in the regulation of milk production and milk quality improvement in buffalo.

Section: Candidate Dgat Genes Affecting Milk Production Traits In Bufmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide identification of Diacylglycerol Acyltransferases (DGAT) family genes influencing Milk production in Buffalo

et al. 2020

BMC Genet

“…Analysis in Anatolian buffalo provided evidence that fixed allele concerning DGAT1 was responsible for the high milk fat yield [12].…”

Section: Candidate Dgat Genes Affecting Milk Production Traits In Bufmentioning

confidence: 99%

Genome-wide identification of diacylglycerol acyltransferases (DGAT) family genes influencing milk production traits in buffalo

et al. 2019

Preprint

Background: The diacylglycerol acyltransferases (DGAT) are a vital group of enzymes in catalyzing triacylglycerol biosynthesis. DGAT genes like DGAT1 and DGAT2 , have been identified as two functional candidate genes affecting milk production traits, especially for fat content in milk. Buffalo milk is famous for its excellent quality, which is rich in fat and protein content. Therefore, this study aimed to characterize DGAT family genes in buffalo and to find candidate markers or DGAT genes influencing lactation performance. Results: We performed a genome-wide study and identified eight DGAT genes in buffalo. All the DGAT genes classified into two distinct clades (DGAT1 and DGAT2 subfamily) based on their phylogenetic relationships and structural features. Chromosome localization displayed eight buffalo DGAT genes distributed on five chromosomes. Collinearity analysis revealed that the DGAT family genes were extensive homologous between buffalo and cattle. Afterward, we discovered genetic variants loci within the genomic regions that DGAT genes located in buffalo. Seven haplotype blocks were constructed and were associated with buffalo milk production traits. Single marker association analyses revealed four most significant single nucleotide polymorphisms (SNPs) mainly affecting milk protein percentage or milk fat yield in buffalo. Genes functional analysis indicated that these DGAT family genes could influence lactation performance in the mammal through regulating lipid metabolism. Conclusion: In the present study, we performed a comprehensive analysis for the DGAT family genes in buffalo, which including identification, structural characterization, phylogenetic classification, chromosomal distribution, collinearity analysis, association analysis, and functional analysis. These findings provide useful information for an in-depth study to determine the role of DGAT family gens play in the regulation of milk production and milk quality improvement in buffalo.

“…To identify the DGAT family members, we used 21 verified DGAT amino acid sequences from bovine (Bos taurus, 3), human (Homo sapiens, 9), mouse (Mus musculus, 7) and rat (Rattus norvegicus, 2) as the query for genome-wide detection of the homologous sequences (Additional file 1). As a result, 24 non-redundant protein sequences encoded by eight DGAT genes (DGATs), including DGAT1, DGAT2, DGAT2L1/MOGAT1, DGAT2L3/AWAT1, DGAT2L4/AWAT2, DGAT2L5/MOGAT2, DGAT2L7/MOGAT3, and DGAT2L6 were identified in Bubalus bubalis (Table 1).…”

Section: Identification Of the Members In The Dgat Familymentioning

confidence: 99%

“…Polymorphisms of the DGAT1 gene were found to be fixed in some buffalo breeds, which were considered to be the primary responsibility for the high milk fat in buffalo [3,8,9]. However, it's hard to credit most of the effects to one single gene DGAT1, since the milk production traits are polygenic traits, a series of genes may be involved in the process, especially in genes belonging to the same family, which have similar sequence and likely common evolutionary origin and similar function.…”

mentioning

confidence: 99%

Genome-wide identification of diacylglycerol acyltransferases (DGAT) family genes influencing milk production traits in buffalo

et al. 2019

Preprint

Background: The diacylglycerol acyltransferases (DGAT) are a vital group of enzymes in catalyzing triacylglycerol biosynthesis. DGAT genes like DGAT1 and DGAT2 , have been identified as two functional candidate genes affecting milk production traits, especially for fat content in milk. Buffalo milk is famous for its excellent quality, which is rich in fat and protein content. Therefore, this study aimed to characterize DGAT family genes in buffalo and to find candidate markers or DGAT genes influencing lactation performance.Results: We performed a genome-wide detection and identified eight DGAT genes in buffalo. All the DGAT genes classified into two distinct clades (DGAT1 and DGAT2 subfamily) based on their phylogenetic relationships and structural features. Chromosome localization displayed eight buffalo DGAT genes distributed on five chromosomes. Collinearity analysis revealed that the DGAT family genes were extensive homologous between buffalo and cattle. Afterward, we discovered genetic variants loci within the genomic regions that DGAT genes located in buffalo. Eight haplotype blocks were constructed and were associated with buffalo milk production traits. Single marker association analyses revealed four most significant single nucleotide polymorphisms (SNPs) mainly affecting milk protein percentage or milk fat yield in buffalo. Genes functional analysis indicated that these DGAT family genes could influence lactation performance in the mammal through regulating lipid metabolism.Conclusion: In the present study, we performed a comprehensive analysis for the DGAT family genes in buffalo, which including identification, structural characterization, phylogenetic classification, chromosomal distribution, collinearity analysis, association analysis, and functional analysis. These findings provide useful information for an in-depth study to determine the role of DGAT family gens play in the regulation of milk production and milk quality improvement in buffalo.