DHA-supplemented diet increases the survival of rats following asphyxia-induced cardiac arrest and cardiopulmonary bypass resuscitation

Kim, Junhwan; Yin, Tai; Shinozaki, Koichiro; Lampe, Joshua W.; Becker, Lance B.

doi:10.1038/srep36545

Cited by 8 publications

(12 citation statements)

References 49 publications

(63 reference statements)

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“…The rats were intubated, mechanically ventilated, and instrumented under anesthesia with 2% isoflurane as described previously [ 15 – 19 ]. End-tidal carbon dioxide (EtCO 2 ) was maintained at 40 ± 5 mmHg during the experiment.…”

Section: Methodsmentioning

confidence: 99%

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Hydrogen gas with extracorporeal cardiopulmonary resuscitation improves survival after prolonged cardiac arrest in rats

et al. 2021

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Background Despite the benefits of extracorporeal cardiopulmonary resuscitation (ECPR) in cohorts of selected patients with cardiac arrest (CA), extracorporeal membrane oxygenation (ECMO) includes an artificial oxygenation membrane and circuits that contact the circulating blood and induce excessive oxidative stress and inflammatory responses, resulting in coagulopathy and endothelial cell damage. There is currently no pharmacological treatment that has been proven to improve outcomes after CA/ECPR. We aimed to test the hypothesis that administration of hydrogen gas (H2) combined with ECPR could improve outcomes after CA/ECPR in rats. Methods Rats were subjected to 20 min of asphyxial CA and were resuscitated by ECPR. Mechanical ventilation (MV) was initiated at the beginning of ECPR. Animals were randomly assigned to the placebo or H2 gas treatment groups. The supplement gas was administered with O2 through the ECMO membrane and MV. Survival time, electroencephalography (EEG), brain functional status, and brain tissue oxygenation were measured. Changes in the plasma levels of syndecan-1 (a marker of endothelial damage), multiple cytokines, chemokines, and metabolites were also evaluated. Results The survival rate at 4 h was 77.8% (7 out of 9) in the H2 group and 22.2% (2 out of 9) in the placebo group. The Kaplan–Meier analysis showed that H2 significantly improved the 4 h-survival endpoint (log-rank P = 0.025 vs. placebo). All animals treated with H2 regained EEG activity, whereas no recovery was observed in animals treated with placebo. H2 therapy markedly improved intra-resuscitation brain tissue oxygenation and prevented an increase in central venous pressure after ECPR. H2 attenuated an increase in syndecan-1 levels and enhanced an increase in interleukin-10, vascular endothelial growth factor, and leptin levels after ECPR. Metabolomics analysis identified significant changes at 2 h after CA/ECPR between the two groups, particularly in d-glutamine and d-glutamate metabolism. Conclusions H2 therapy improved mortality in highly lethal CA rats rescued by ECPR and helped recover brain electrical activity. The underlying mechanism might be linked to protective effects against endothelial damage. Further studies are warranted to elucidate the mechanisms responsible for the beneficial effects of H2 on ischemia–reperfusion injury in critically ill patients who require ECMO support.

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Section: Methodsmentioning

confidence: 99%

“…This study used a rat model of highly lethal prolonged asphyxia‐induced CA, as reported previously [ 15 – 19 ]. In our previous studies, the survival rate was ~ 20% at 4 h after CA/ECPR [ 19 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

Hydrogen gas with extracorporeal cardiopulmonary resuscitation improves survival after prolonged cardiac arrest in rats

et al. 2021

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“…Lipids are fundamentally important for energy storage, cell membrane structure and cellular functions (Sunshine & Iruela‐Arispe, 2017). Studies suggest that within lipids, the omega‐3 (n–3) polyunsaturated fatty acids (PUFA), in particular alpha‐linolenic acid (ALA; 18:3n‐3), eicosapentaenoic acid (EPA; 20:5n‐3), and docosahexaenoic acid (DHA; 22:6n‐3), can be important for somatic development, especially for nervous and gonadal tissues (Arts & Kohler, 2009; Guo et al, 2016a; Tocher et al, 2019), cognition (Cunanne et al, 2009; Hoffman et al, 2009; McCann & Ames, 2005), reproduction (Chen et al, 2012; Martin‐Creuzburg et al, 2009; Roqueta‐Rivera et al, 2010; Sinendo et al, 2017) and survival (Fuiman & Perez, 2015; Kim et al, 2016; Matsunari et al, 2013; Mesa‐Rodriguez et al, 2018; Twining et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Adaptation, behavior, diversification, genetics, metabolic networks, nutritional landscapes, omega-3 polyunsaturated fatty acids, traits 2009; Hoffman et al, 2009;McCann & Ames, 2005), reproduction (Chen et al, 2012;Roqueta-Rivera et al, 2010;Sinendo et al, 2017) and survival (Fuiman & Perez, 2015;Kim et al, 2016;Matsunari et al, 2013;Mesa-Rodriguez et al, 2018;.…”

Section: Introductionmentioning

confidence: 99%

The evolutionary ecology of fatty‐acid variation: Implications for consumer adaptation and diversification

et al. 2021

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The nutritional diversity of resources can affect the adaptive evolution of consumer metabolism and consumer diversification. The omega‐3 long‐chain polyunsaturated fatty acids eicosapentaenoic acid (EPA; 20:5n‐3) and docosahexaenoic acid (DHA; 22:6n‐3) have a high potential to affect consumer fitness, through their widespread effects on reproduction, growth and survival. However, few studies consider the evolution of fatty acid metabolism within an ecological context. In this review, we first document the extensive diversity in both primary producer and consumer fatty acid distributions amongst major ecosystems, between habitats and amongst species within habitats. We highlight some of the key nutritional contrasts that can shape behavioural and/or metabolic adaptation in consumers, discussing how consumers can evolve in response to the spatial, seasonal and community‐level variation of resource quality. We propose a hierarchical trait‐based approach for studying the evolution of consumers’ metabolic networks and review the evolutionary genetic mechanisms underpinning consumer adaptation to EPA and DHA distributions. In doing so, we consider how the metabolic traits of consumers are hierarchically structured, from cell membrane function to maternal investment, and have strongly environment‐dependent expression. Finally, we conclude with an outlook on how studying the metabolic adaptation of consumers within the context of nutritional landscapes can open up new opportunities for understanding evolutionary diversification.

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“…The experimental protocol was approved by the Institutional Animal Care and Use Committee of the University of Pennsylvania (#804889). The detailed surgical procedures were provided in supplementary materials [13]. Male adult Sprague-Dawley rats were used.…”

Section: Asphyxial Ca and Cpb Resuscitationmentioning

confidence: 99%

Increased Survival Time With SS-31 After Prolonged Cardiac Arrest in Rats

Zhang

Tam

Shinozaki

et al. 2019

Heart, Lung and Circulation

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Cardiac arrest is one of the leading causes of death with a very high mortality rate. No therapeutic drug that can be administered during resuscitation has been reported. Mitochondrial dysfunction is believed to play an important role for the pathogenesis of cardiac arrest. SS-31, a tetra-peptide, has been shown to protect mitochondria from ischaemia/reperfusion injury. Therefore, we tested whether SS-31 improves rat survival after prolonged cardiac arrest. Rats were randomised into two groups. After 25minutes of asphyxia-induced cardiac arrest, rats were resuscitated with or without SS-31 using cardiopulmonary bypass resuscitation. Rat survival was followed for additional 4.5hours using haemodynamic monitoring. The blood gas was analysed for surviving rats at multiple time points. After 5hours, 5 of 10 rats survived in the SS-31 group whereas only 1 of 10 rats survived in the control group (p=0.026). At 90minutes after resuscitation, the blood lactate level in the SS-31 treated rats (4.29±2.5mmol/L) was significantly lower than in control rats (7.36±3.1mmol/L, p=0.026), suggesting mitochondrial aerobic respiration was improved with SS-31 treatment. Overall, our data show the potential of SS-31 as a novel therapeutic in cardiac arrest.

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DHA-supplemented diet increases the survival of rats following asphyxia-induced cardiac arrest and cardiopulmonary bypass resuscitation

Cited by 8 publications

References 49 publications

Hydrogen gas with extracorporeal cardiopulmonary resuscitation improves survival after prolonged cardiac arrest in rats

Hydrogen gas with extracorporeal cardiopulmonary resuscitation improves survival after prolonged cardiac arrest in rats

The evolutionary ecology of fatty‐acid variation: Implications for consumer adaptation and diversification

Increased Survival Time With SS-31 After Prolonged Cardiac Arrest in Rats

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