Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells

Tripathi, Anima; Pandey, Vivek; Sahu, Alakh N.; Singh, Alok Kumar; Dubey, Pawan K.

doi:10.1039/c8tx00263k

Cited by 75 publications

(42 citation statements)

References 50 publications

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“…Di-2-ethylhexyl phthalate exposure has been previously linked to increased ROS production in somatic cells and oocytes (Ambruosi et al, 2011;Kim et al, 2013;Wu et al, 2014;Tripathi et al, 2019). However, its impact on ROS overproduction in sperm was not elucidated.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility

Khasin

Rosa

Petersen

et al. 2020

Front. Cell Dev. Biol.

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A growing number of studies point to reduced fertility upon chronic exposure to endocrine-disrupting chemicals (EDCs) such as phthalates and plasticizers. These toxins are ubiquitous and are often found in food and beverage containers, medical devices, as well as in common household and personal care items. Animal studies with EDCs, such as phthalates and bisphenol A have shown a dose-dependent decrease in fertility and embryo toxicity upon chronic exposure. However, limited research has been conducted on the acute effects of these EDCs on male fertility. Here we used a murine model to test the acute effects of four ubiquitous environmental toxins: bisphenol A (BPA), di-2-ethylhexyl phthalate (DEHP), diethyl phthalate (DEP), and dimethyl phthalate (DMP) on sperm fertilizing ability and pre-implantation embryo development. The most potent of these toxins, di-2-ethylhexyl phthalate (DEHP), was further evaluated for its effect on sperm ion channel activity, capacitation status, acrosome reaction and generation of reactive oxygen species (ROS). DEHP demonstrated a profound hazardous effect on sperm fertility by producing an altered capacitation profile, impairing the acrosome reaction, and, interestingly, also increasing ROS production. These results indicate that in addition to its known chronic impact on reproductive potential, DEHP also imposes acute and profound damage to spermatozoa, and thus, represents a significant risk to male fertility.

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Section: Discussionmentioning

confidence: 99%

The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility

Khasin

Rosa

Petersen

et al. 2020

Front. Cell Dev. Biol.

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“…In addition, glutathione, transferase and catalase in ovarian follicular fluid are significantly decreased in older women undergoing in vitro fertilization ( 25 ). Therefore, oxidative stress induces granulosa cell discordant function and influences oocyte quality ( 28 ).…”

Section: Discussionmentioning

confidence: 99%

Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H₂O₂

Li¹,

Kang

et al. 2020

Exp Ther Med

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“…There are two main ways in which sperm can encounter unmetabolized DEHP: via abrupt release from adipose tissue or through release from medical devices, such as a urological catheter-making unmetabolized DEHP a prominent threat to sperm fertility. DEHP exposure has been previously linked to increased ROS production in somatic cells and oocytes (Wu et al 2014;Tripathi et al 2019;Kim et al 2013;Ambruosi et al 2011). However, its impact on ROS over-production in sperm was not elucidated.…”

Section: Discussionmentioning

confidence: 99%

The impact of di-2-ethylhexyl phthalate on sperm fertility

Khasin

Rosa

Petersen

et al. 2020

Preprint

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A growing number of studies point to reduced fertility upon chronic exposure to endocrine-disrupting chemicals (EDCs) such as phthalates and plasticizers. These toxins are ubiquitous and are often found in food and beverage containers, medical devices, as well as in common household and personal care items. Animal studies with EDCs, such as phthalates and bisphenol A have shown a dose-dependent decrease in fertility and embryo toxicity upon chronic exposure. However, limited research has been conducted on the acute effects of these EDCs on male fertility. Here we used a murine model to test the acute effects of four ubiquitous environmental toxins: bisphenol A (BPA), di-2-ethylhexyl phthalate (DEHP), diethyl phthalate (DEP), and dimethyl phthalate (DMP) on sperm fertilizing ability and pre-implantation embryo development. The most potent of these toxins, di-2ethylhexyl phthalate (DEHP), was further evaluated for its effect on sperm ion channel activity, capacitation status, acrosome reaction and generation of reactive oxygen species (ROS). DEHP demonstrated a profound hazardous effect on sperm fertility by producing an altered capacitation profile, impairing the acrosome reaction, and, interestingly, also increasing ROS production. These results indicate that in addition to its known chronic impact on reproductive potential, DEHP also imposes acute and profound damage to spermatozoa, and thus, represents a significant risk to male fertility.

show abstract

Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells

Abstract: Increased oxidative stress (OS) due to ubiquitous exposure to di-(2-ethylhexyl) phthalate (DEHP) can affect the quality of oocytes by inducing apoptosis and hampering granulosa cell mediated steroidogenesis.

Cited by 75 publications

References 50 publications

The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility

The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility

Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H₂O₂

The impact of di-2-ethylhexyl phthalate on sperm fertility

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Di-(2-ethylhexyl) phthalate (DEHP) inhibits steroidogenesis and induces mitochondria-ROS mediated apoptosis in rat ovarian granulosa cells

Abstract: Increased oxidative stress (OS) due to ubiquitous exposure to di-(2-ethylhexyl) phthalate (DEHP) can affect the quality of oocytes by inducing apoptosis and hampering granulosa cell mediated steroidogenesis.

Cited by 75 publications

References 50 publications

The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility

The Impact of Di-2-Ethylhexyl Phthalate on Sperm Fertility

Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H2O2

The impact of di-2-ethylhexyl phthalate on sperm fertility

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Product

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Salvia miltiorrhiza solution and its active compounds ameliorate human granulosa cell damage induced by H₂O₂