Diabetes and multidrug-resistance gene mutation: tuberculosis in Zunyi, Southwest China

Abstract

Background: The aim of this study was to clarify the characteristics of gene mutation related to multidrug-resistance (MDR) of tuberculosis (TB) and diabetes in Zunyi.Methods: A total 763 patients with TB were screened for TB-DNA, TB-RNA, and acid-fast staining (all were positive). They were divided into the tuberculosis (TB) group and the diabetes mellitus-tuberculosis (DM-TB) group. We compared and analyzed the MDR gene rpoB, KatG, and inhA characteristics of gene mutations in the two groups by polymerase chain reaction (PCR)-reverse dot hybridization, and collected relevant clinical data to explore its correlation with the occurrence of multidrug resistance.Results: Multidrug resistance occurred in 32 of the 525 patients in the TB group, and extensive drug resistance occurred in 15 of the 207 patients in the DM-TB group. In the DM-TB group, the mutation rates of ropBS531L and ropB531 (both 53.33%) were lower than those of the TB group (both 59.38%) in rifampicin resistance mutations. Most of the mutations were at the KatG315N site, conferring isoniazid resistance. Conclusions:The mutation sites of multidrug-resistant patients in Zunyi are mainly ropB531 and ropBS531L mutations, which are prone to co-occurrence; patients with MDR-TB alone are prone to mutations at the KatG315N site, while patients with diabetes and MDR-TB are more likely to have inhA-15M site mutations.

Analysis of Drug Resistance Characteristics of Mycobacterium Tuberculosis in Anhui Province, 2016-2022

“…Within the Mycobacterium tuberculosis strains coexistent with diabetes, statistical differences were only noted in the resistance rates to RFP and in the multidrug resistance (MDR) rates, while other anti-tuberculosis drugs showed no statistical variance. Moreover, strains from populations with and without concurrent diabetes exhibited statistically significant differences in resistance rates across various ages, genders, categories, and income groups, aligning with the research conducted by Mu Lin et al [22][23][24] In summary, the tuberculosis drug-resistance monitoring results in Anhui province reveal that following the implementation of the 13th Five-Year Plan for Tuberculosis Control and the 2019-2022 Action Plan to Curb Tuberculosis, the drug resistance rates of Mycobacterium tuberculosis have been somewhat controlled. However, due to the still large base of pulmonary tuberculosis patients, the incidence of drug-resistant tuberculosis remains relatively high.…”

“…Within the Mycobacterium tuberculosis strains coexistent with diabetes, statistical differences were only noted in the resistance rates to RFP and in the multidrug resistance (MDR) rates, while other anti-tuberculosis drugs showed no statistical variance. Moreover, strains from populations with and without concurrent diabetes exhibited statistically significant differences in resistance rates across various ages, genders, categories, and income groups, aligning with the research conducted by Mu Lin et al 22–24 …”

The Detection of Mutations and Genotyping of Drug-Resistant Mycobacterium tuberculosis Strains Isolated from Patients in the Rural Eastern Cape Province

“…This high frequency of occurrence may be due to the low fitness cost associated with rpoB S531L, [ 31 ] and has been associated with major MDR-TB outbreaks. [ 32 ]. A low frequency of mutation was observed in codon 526, at 3.8% in this study, but is higher in Uganda, at 12.5% [ 33 ]; China, at 14.9%; [ 27 ] and Brazil, at 9.9% [ 34 ]; this indicates that frequency varies according to geographic location.…”