Although key factors of online health information-seeking behavior (OHISB), such as self-efficacy, Internet experience, and perceived ease of use, are analyzed in many studies, the research results are controversial. The purpose of this meta-analysis, based on 27 related empirical studies, is to explore the determinants of OHISB. The determinants of OHISB are classified into four categories: demographic characteristic factors, cognitive factors, internal factors, and external factors. According to the results of the analysis using Stata13.0, our study found a weak effect of perceived cost and health anxiety on the OHISB, while subjective norm, perceived usefulness, and attitude have a strong positive effect on the OHISB. Understanding the determinants of OHISB is beneficial in order to know why users utilize online health applications. The findings of the study can contribute to developing and extending the existing theoretical concepts.