The Schiff-base compound N,N′-Bis-(2,5-dimethoxybenzylidene)-1,3-diaminopropan, (2,5-MeO-ba) 2 pn, is synthesized and characterized by elemental analysis, FT-IR and 1 H NMR spectroscopy. The crystal structure of (2,5-MeO-ba) 2 pn is determined by X-ray single crystal diffraction analysis. The X-ray diffraction analysis at 120 K reveals that (2,5-MeO-ba) 2 pn crystallizes in the orthorhombic system, Fdd2 space group with a = 29.339(9) Å, b = 29.4073(11) Å, c = 4.4524(2) Å, V = 3840.8(3) Å 3 , μ = 0.72 mm -1 , and Z = 8. Keywords: X-ray diffraction, orthorhombic symmetry, Schiff base.Schiff bases derived from substituted benzaldehyde [1-3] are widely used as chelating ligands forming transition metal complexes [4][5][6]. They have attracted considerable attention in the last years because the complexes are very interesting in many fields, such as catalysis [7] and magnetism [8]. Recently, several Schiff-base compounds with antibacterial activity have been investigated [9,10]. In this paper, the title compound (2,5-MeO-ba) 2 pn was synthesized and its molecular structure was characterized by elemental analysis, FT-IR and 1 H NMR spectroscopy, and X-ray structure analysis (Fig. 1).Experimental. All reagents and solvents for the synthesis and spectroscopic studies were commercially available and used as received without further purification. 1 H NMR spectra were measured with a BRUKER DRX-500 AVANCE spectrometer at 500 MHz and all chemical shifts are reported in δ units downfield from TMS. The infrared spectrum was recorded on a JASCO 680 plus FT-IR spectrophotometer as a KBr pellet.2,5-Dimethoxybenzaldehyde (0.332 g, 0.2 mmol) and 1,3-propanediamine (0.074 g, 0.1 mmol) were dissolved in ethanol (50 ml). The mixture was stirred at room temperature for 1 h to give a clear solution. Suitable crystals of the title compound (2,5-MeO-ba) 2 pn for the X-ray study were formed by slow evaporation of the solvent over 8 days at room temperature (yield 76%). The crystals are colorless. IR (KBr pellet, cm -1 ): 2829-3008 (m, C-H aromatic and aliphatic), 1638 Fig. 1. Chemical diagram of the title compound (2,5-MeO-ba) 2 pn.