Diacyllipid Micelle-Based Nanocarrier for Magnetically Guided Delivery of Drugs in Photodynamic Therapy

Abstract

We report the design, synthesis using nanochemistry, and characterization of a novel multifunctional polymeric micelle-based nanocarrier system, which demonstrates combined function of magnetophoretically guided drug delivery together with light-activated photodynamic therapy. Specifically, the nanocarrier consists of polymeric micelles of diacylphospholipid-poly(ethylene glycol) (PE-PEG) coloaded with the photosensitizer drug 2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a (HPPH), and magnetic Fe3O4 nanoparticles. The nanocarrier shows excellent stability and activity over several weeks. The physicochemical characterizations have been carried out by transmission electron micrography and optical spectroscopy. An efficient cellular uptake has been confirmed with confocal laser scanning microscopy. The loading efficiency of HPPH is practically unaffected upon coloading with the magnetic nanoparticles, and its phototoxicity is retained. The magnetic response of the nanocarriers was demonstrated by their magnetically directed delivery to tumor cells in vitro. The magnetophoretic control on the cellular uptake provides enhanced imaging and phototoxicity. These multifunctional nanocarriers demonstrate the exciting prospect offered by nanochemistry for targeting photodynamic therapy.

Photodynamic therapy of experimental B-16 melanoma in mice with tumor-targeted 5,10,15,20-tetraphenylporphin-loaded PEG-PE micelles

“…The average effective diameters of the PEG-PE micelles were 10 nm with a narrow size distribution. The zeta potential measurements showed an overall net negative surface charge of the all micelle samples (26 -30 mV), similar to those reported by other authors (Neradovic et al 2001;Cinteza et al 2006).…”

Improvement of drug safety by the use of lipid-based nanocarriers

“…In a different study, liposomes incorporating chlorin e6 (Ce6) trimethylester (for photodynamic therapy of cancer) was shown to lower the LC 80 by approximately 53 folds in gastric cancer cells compared to Ce6 sodium salt and caused complete tumor remission in animal with gastric cancer [93]. m-PEG lipid micelles incorporating hydrophobic benzoporphyrin derivatives, tetraphenylporphin and pyropheophorbide derivatives have also been investigated for photodynamic therapy of cancer [94][95][96]. A recent review by Nadezda et al gives a good overview of mechanisms of photoresponsive release of drugs from nanocarriers [97].…”

Superparamagnetic iron oxide nanoparticles: magnetic nanoplatforms as drug carriers

“…94 The development of multifunctional magnetic nanoparticle formulations further boosts the potential use of SPIONs in medicine. These formulations not only serve the purpose of being drug delivery vectors but also have applications in MRI imaging, 95 targeted thermosensitive chemotherapy, 96 magnetically targeted photodynamic therapy, 97 and fluorescent/luminescence imaging. 98 Yallapu et al developed water-dispersible SPIONs for hyperthermia, MRI, and drug delivery applications.…”