Diagnosis and Control of Cereal Viruses in the Middle East

Aboul-Ata, Aboul-Ata E.; Mazyad, H. M.; El-Attar, Ahmed K.; Soliman, A.M.; Anfoka, Ghandi; Zeidan, M.; Gorovits, Rena; Sobol, Iris; Czosnek, Henryk

doi:10.1016/b978-0-12-385885-6.00007-9

Cited by 18 publications

(9 citation statements)

References 80 publications

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“…The diagnosis step involves a careful examination to determine the underlying cause of the disease; whereas detection is to find out the virus. Accurate detection and diagnosis of plant virus diseases is the initially vital step for the crop management system (Aboul-Ata et al, 2011;Adams et al, 2013). Since after virus infection, chemical treatments/application to plants do not lead to effective control measures, plant viral diseases most effectively managed as control measures are applied before infection (Aboul-Ata et al, 2011).…”

Section: Plant Virus Diagnosismentioning

confidence: 99%

Advanced and Commonly Used Serological Techniques for Detection and Diagnosis of Plant Virus: Review

2022

ALST

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Plant diseases caused by viruses are the most menace to sustainable agriculture, leading to numerous billion dollars in losses annually. Proper and accurate detection of plant viruses is always the key to developing appropriate solutions to manage the economic losses caused by them. Advances in technology have simplified the tools available to detect and diagnose viruses at a time when control measures should be used. Currently, nucleic acid and serological based diagnostic methods are widely used for plant viral identifications. Serological techniques which based on antibody and antigen reaction is broadly used for the detection of plant viruses because of its simplicity, adaptability and sensitivity. A large number of advanced serological tests are available such as enzymelinked immunosorbent assay (ELISA), dot immunobinding assay, tissue blotting immunoassay and Lateral flow assay. Various ELISA varieties such as double-antibody sandwich-ELISA, triple-antibody sandwich-ELISA, and antigen-coated-ELISA are widely used in the detection and testing of plant viruses. Dot immunobinding assay performed on a nitrocellulose that the plant extracts are spotted onto a membrane while tissue blotting immunoassay is an easy method that the crude sap from plants is directly blotted and used for detection through immunology. Lateral flow assay is the latest and the only serological method used for the detection of viruses without the aid of laboratory and skilled personnel. It is an onsite and quick assay that can be performed by anyone and suitable for the detection of viruses that occur in high concentrations in plants.

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Section: Plant Virus Diagnosismentioning

confidence: 99%

Advanced and Commonly Used Serological Techniques for Detection and Diagnosis of Plant Virus: Review

2022

ALST

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“…PVX, genus Potexvirus, belongs to Alphaflexiviridae family and is a well-known potato crop pathogen globally. It causes an infection widely in potato plant host, specifically in family Solanaceae (Aboul-Ata et al 2011;King et al 2011). Both the concerted metabolites and their acetone source which is an Egyptian isolate (Streptomyces afghanensis) inhibited the local wound development triggered by PVX disease cv.…”

Section: Potato Virus X (Pvx)mentioning

confidence: 99%

Plant Growth-Promoting Rhizobacteria (PGPR) as Biocontrol Agents for Viral Protection

Basıt

Shah

Muntha

et al. 2021

Plant Growth-Promoting Microbes for Sustainable Biotic and Abiotic Stress Management

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“…Accurate diagnosis of virus diseases, is the first important step for crop management system (Aboul-Ata et al, 2011). Since after virus infection, agrochemical treatments to plants do not lead to an effective control, viral diseases most effectively managed as control measures are applied before infection (Aboul-Ata et al, 2011). In order to prevent plant viral diseases, it is important to figure out the causes and to distinguish diseased plants and unaffected plants that show viruslike symptoms (Pearson et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…As mentioned above, unlike other plant pathogens, the management of plant viral diseases based on direct methods have not been developed yet, so that viral diseases can be controlled by indirect strategies such as insect viral vector control or removing diseased plants (Aboul-Ata et al, 2011;Wang et al, 2011). The methods for detection and identification of viruses are critical in virus disease management (Aboul-Ata et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

A Review of Detection Methods for the Plant Viruses

Jeong¹,

Ju²,

Noh³

2014

Research in Plant Disease

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The early and accurate detection of plant viruses is an essential component to control those. Because the globalization of trade by free trade agreement (FTA) and the rapid climate change promote the country-tocountry transfer of viruses and their hosts and vectors, diagnosis of viral diseases is getting more important. Because symptoms of viral diseases are not distinct with great variety and are confused with those of abiotic stresses, symptomatic diagnosis may not be appropriate. From the last three decades, enzyme-linked immunosorbent assays (ELISAs), developed based on serological principle, have been widely used. However, ELISAs to detect plant viruses decrease due to some limitations such as availability of antibody for target virus, cost to produce antibody, requirement of large volume of sample, and time to complete ELISAs. Many advanced techniques allow overcoming demerits of ELISAs. Since the polymerase chain reaction (PCR) developed as a technique to amplify target DNA, PCR evolved to many variants with greater sensitivity than ELISAs. Many systems of plant virus detection are reviewed here, which includes immunological-based detection system, PCR techniques, and hybridization-based methods such as microarray. Some of techniques have been used in practical, while some are still under developing to get the level of confidence for actual use.

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Diagnosis and Control of Cereal Viruses in the Middle East

Cited by 18 publications

References 80 publications

Advanced and Commonly Used Serological Techniques for Detection and Diagnosis of Plant Virus: Review

Advanced and Commonly Used Serological Techniques for Detection and Diagnosis of Plant Virus: Review

Plant Growth-Promoting Rhizobacteria (PGPR) as Biocontrol Agents for Viral Protection

A Review of Detection Methods for the Plant Viruses

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